Association of Single Nucleotide Polymorphisms in the Human Tumor Necrosis Factor-α and Interleukin 1-β Genes in Patients with Pre-eclampsia
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Abstract
Pre-eclampsia is a pregnancy-specific syndrome that may be dangerous especially to the fetus. Different cytokines have been found to be elevated in women with pre-eclampsia and may have possible roles in the development of this disorder. Alleles of the interleukin-l-beta (IL-lβ) and tumor necrosis factor alpha (TNF-α) genes are associated with pr-eeclampsia in several studies in different populations. The aim of the present study was to investigate the relationship between IL-lβ (C+3954T) and TNF-α (G-308A) gene polymorphisms with preeclampsia in north east of Iran (Khorasan province).
This study included 54 diagnosed patients with pre-eclampsia and 50 normal pregnant women as control group. DNA was extracted from peripheral blood and the polymorphisms were determined by PCR-RFLP method. Data was analyzed using chi-square and Fisher’s exact tests.
There was significant association between TNF-α (G-308A) genotype and pre-eclampsia (p=0.001) but we did not find any significant association between IL-lβ (C+3954T) genotype and pre-eclampsia (p=0.39).
The present study might suggest a role for TNF-α in the development of pre-eclampsia; however, IL-lβ (C+3954T) polymorphism could not be considered as a marker of susceptibility to preeclampsia in our population.
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| Files | ||
| Issue | Vol 11, No 3 (2012) | |
| Section | Articles | |
| Keywords | ||
| Gene Polymorphism Interleukine-1β PCR-RFLP Pre-eclampsia Tumor Necrosis Factor-α | ||
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