Brief Communication
  

Association of Toxoplasmosis with Serum TGF-β, IL-17, and IL-6 Levels in Individuals with Diabetes

Abstract

Cytokines play an essential role in regulating the interaction of immune cells in diabetes and infections such as toxoplasmosis. The purpose of this study was to examine the serum levels of interleukin (IL)-6, IL-17, and transforming growth factor-beta (TGF-β) in patients with type 1 and type 2 diabetes mellitus with toxoplasmosis, and to explore their inter-relationship.
Forty patients with diabetes mellitus, including 20 with type 1 and 20 with type 2, as well as 20 healthy subjects, voluntarily participated in the study. Each subject provided 5 mL of peripheral blood for enzyme-linked immunosorbent assay.
Subjects with type 2 diabetes mellitus who also had toxoplasmosis showed a significant increase in TGF-β levels and a decrease in IL-6 levels. In contrast, patients with type 1 diabetes mellitus displayed a slight increase in IL-6 and IL-17 levels compared to both the patients with type 2 diabetes and the healthy control group.
Our findings show an increase in TGF-β and a decrease in IL-6, which may suggest a reduction in inflammation and beta cell destruction in individuals with type 2 diabetes and toxoplasmosis. The elevated serum levels of IL-6 and IL-17 in individuals with type 1 diabetes further support the exacerbation of inflammation.

1. Tosur M, Geyer SM, Rodriguez H, Libman I, Baidal DA, Redondo MJ. Ethnic differences in progression of islet autoimmunity and type 1 diabetes in relatives at risk. Diabetologia. 2018;61(7):2043-53.
2. Coman LI, Coman OA, Bădărău IA, Păunescu H, Ciocîrlan M. Association between liver cirrhosis and diabetes mellitus: A review on hepatic outcomes. J Clin Med. 2021;10(4):1–16.
3. Zatterale F, Longo M, Naderi J, Raciti GA, Desiderio A, Miele C BF. Chronic Adipose Tissue Inflammation Linking Obesity to Insulin Resistance and Type 2 Diabetes. Front Physiol. 2020;10(5):1607–27.
4. Mensah-Brown EPK, Shahin A, Al-Shamisi M, Wei X, Lukic ML. IL-23 leads to diabetes induction after subdiabetogenic treatment with multiple low doses of streptozotocin. Eur J Immunol. 2006;36(8):216–23.
5. Oliveira-Scussel AC, Ferreira PT, Resende RD, Ratkevicius-Andrade CM, Gomes AD, Paschoini MC, De Vito FB, et al. Association of gestational diabetes mellitus and negative modulation of the specific humoral and cellular immune response against Toxoplasma gondii. Front Immunol. 2022;13(1):1–18.
6. Andrade MMC, Carneiro VL, Galvão AA, Fonseca TR, Vitor RWA, Alcantara-Neves NM, et al. Toxoplasma gondii protects from IgE sensitization and induces Th1/Th2 immune profile. Parasite Immunol. 2020;42:1–8.
7. Beshay EV, El-Refai SA, Helwa MA, Atia AF, Dawoud MM. Toxoplasma gondii as a possible causative pathogen of type-1 diabetes mellitus: Evidence from case-control and experimental studies. Exp Parasitol. 2018;188(16):93–101.
8. Nosaka K, Hunter M WW. The role of Toxoplasma gondii as a possible inflammatory agent in the pathogenesis of type 2 diabetes mellitus in humans. Fam Med Community Heal. 2016;4(2):44–62.
9. Abdel-Moneim A, Bakery HH, Allam G. The potential pathogenic role of IL-17/Th17 cells in both type 1 and type 2 diabetes mellitus. Biomed Pharmacother. 2018;4(5):287–92.
10. Shabib Mohsen B, Abood Farhan A, Abdul-Daim Saleh M. Evaluation Of the Immunopatho Role Of Interleukins IL17, IL21,and CD4+,CD8+ T cells In Patients With Type 1 Diabetes In The City of Baquba. Diyala J Med. 2018;14(3):110–7.
11. Roohi A, Tabrizi M, Abbasi F, Ataie-Jafari A, Nikbin B, Larijani B, et al. Serum IL-17, IL-23, and TGF-β levels in type 1 and type 2 diabetic patients and age-matched healthy controls. Biomed Res Int. 2014;18946.
12. S. Li, C. Joseph, C. Becourt, J. Klibi, S. Luce, D. Dubois-Laforgue, et al. Potential role of IL-17-producing iNKT cells in type 1 diabetes. PLoS One 2014;9:e96151.
13. Asgari Q, Motazedian MH, Khazanchin A, Mehrabani D, Naderi Shahabadi S. High Prevalence of Toxoplasma gondii Infection in Type i Diabetic Patients. J Parasitol Res. 2021;2021:1–6.
14. Kikodze N, Pantsulaia I, Kh R, Iobadze M, Dzhakhutashvili N, Pantsulaia N, et al. Cytokines and T regulatory cells in the pathogenesis of type 1 diabetes. Georgian Med News. 2013;222(9):29–35.
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Keywords
Cytokines Diabetes mellitus Immune system Inflammation Toxoplasmosis
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How to Cite
1.
Dabirzadeh M, Ghoryani M, Poursamimi J, Fouladi B. Association of Toxoplasmosis with Serum TGF-β, IL-17, and IL-6 Levels in Individuals with Diabetes. Iran J Allergy Asthma Immunol. 2024;:1-6.