The Association between Inflammatory Cytokines and miRNAs with Slow Coronary Flow Phenomenon
-
Shahla Danaii
,
Sadaf Shiri
,
Sanam Dolati
,
Majid Ahmadi
,
Leila Ghahremani-Nasab
,
Atefeh Amiri
,
Amin Kamrani
,
Hossein Samadi Kafil
,
Forough Chakari-Khiavi
,
Mohammad Hojjat-Farsangi
,
Aida Malek Mahdavi
,
Amir Mehdizadeh
,
Mehdi Yousefi
Abstract
Slow coronary flow (SCF) is a coronary artery disorder. Several inflammatory mediators have been reported to be associated with vascular homeostasis and endothelial dysfunction. The aim of this study was to investigate the association between cytokines and miRNAs in patients with SCF compared to the controls. In this regard, blood samples were acquired from 45 SCF patients and 45 age- and sex-matched healthy control subjects. Serum and peripheral blood mononuclear cells (PBMCs) were separated. Expression levels of miRNAs and cytokines in PBMCs were measured by real-time PCR. As a final point, serum levels of cytokines were quantified by ELISA. Expression levels of miR-1, miR-133, miR-208a, miR-206, miR-17, miR-29, miR-223, miR-326, and miR-155 as considerable indicators of inflammatory function significantly increased in SCF patients while the expression levels of miR-15a, miR-21, miR-25, miR-126, miR-17, miR-16 and miR-18a as considerable indicators of anti-inflammatory function significantly decreased in patients with SCF compared to the control group. Additionally, serum IL-1β, IL‐8, and TNF-α concentrations were significantly higher in the SCF group than controls. However, no significant differences were observed in IL-10 production in SCF patients compared to the controls. This study provided the potential role of miRNAs as biomarkers for SCF diagnosis as well as suitable markers for monitoring coronary artery disease (CAD) development in these patients. More investigations are still necessary to unravel the detailed essential mechanisms of circulating miRNA levels in patients with heart failure and SCF.
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29. Diehl P, Fricke A, Sander L, Stamm J, Bassler N, Htun N, et al. Microparticles: major transport vehicles for distinct microRNAs in circulation. Cardiovascular Research. 2012;93(4):633-44.
30. Guo J-F, Zhang Y, Zheng Q-X, Zhang Y, Zhou H-H, Cui L-M, et al. Association between elevated plasma microRNA-223 content and severity of coronary heart disease. Scandinavian Journal of Clinical and Laboratory Investigation 2018:1-6.
31. Jiang Y, Liu N, Xue B, Hou J, Lin C, Ren J, et al. Circulating MicroRNA Profiles Differ between Hyperglycemia and Euglycemia in Coronary Heart Disease Patients. BioMed Research International. 2017;2017:9192575.
32. Cao Z, Qian L, Wu S. Roles of miR-16 in vascular endothelial injury in patients with coronary heart disease. Biomedical Research. 2017;28(5).
33. Hoekstra M, van der Lans CA, Halvorsen B, Gullestad L, Kuiper J, Aukrust P, et al. The peripheral blood mononuclear cell microRNA signature of coronary artery disease. Biochemical and Biophysical Research Communications. 2010;394(3):792-7.
34. Dolati S, Ahmadi M, Aghebti-Maleki L, Nikmaram A, Marofi F, Rikhtegar R, et al. Nanocurcumin is a potential novel therapy for multiple sclerosis by influencing inflammatory mediators. Pharmacological Reports. 2018;70(6):1158-67.
35. Bernhagen J. Protective cardiac conditioning by an atypical cytokine. Clinical Science. 2019;133(8):933-7.
36. Dolati S, Marofi F, Babaloo Z, Aghebati-Maleki L, Roshangar L, Ahmadi M, et al. Dysregulated Network of miRNAs Involved in the Pathogenesis of Multiple Sclerosis. Biomedicine Pharmacotherapy. 2018;104:280-90.
37. Renjie W, Haiqian L. MiR-132, miR-15a and miR-16 synergistically inhibit pituitary tumor cell proliferation, invasion and migration by targeting Sox5. Cancer letters. 2015;356(2):568-78.
38. Montoya MM, Maul J, Singh PB, Pua HH, Dahlström F, Wu N, et al. A distinct inhibitory function for miR-18a in Th17 cell differentiation. The Journal of Immunology. 2017;199(2):559-69.
2. Nakanishi K, Daimon M. Coronary Slow Flow and Subclinical Left Ventricular Dysfunction. International heart journal. 2019;60(3):495-6.
3. Mukhopadhyay S, Kumar M, Yusuf J, Gupta VK, Tyagi S. Risk factors and angiographic profile of coronary slow flow (CSF) phenomenon in North Indian population: An observational study. Indian heart journal. 2018;70(3):405-9.
4. Arbel Y, Sternfeld A, Barak A, Burgansky-Eliash Z, Halkin A, Berliner S, et al. Inverse correlation between coronary and retinal blood flows in patients with normal coronary arteries and slow coronary blood flow. Atherosclerosis. 2014;232(1):149-54.
5. Mutluer FO, Ural D, Gungor B, Bolca O, Aksu T. Association of Interleukin-1 Gene cluster polymorphisms with coronary slow flow phenomenon. Anatolian journal of cardiology. 2018;19(1):34-41.
6. Linton MF, Yancey PG, Davies SS, Jerome WGJ, Linton EF, Vickers KC. The role of lipids and lipoproteins in atherosclerosis. 2015.
7. Velásquez IM, Frumento P, Johansson K, Berglund A, de Faire U, Leander K, et al. Association of interleukin 8 with myocardial infarction: results from the Stockholm Heart Epidemiology Program. International Journal of Cardiology. 2014;172(1):173-8.
8. Welty FK. How do elevated triglycerides and low HDL-cholesterol affect inflammation and atherothrombosis? Current cardiology reports. 2013;15(9):400.
9. Cavarretta E, Frati GJBri. MicroRNAs in coronary heart disease: ready to enter the clinical arena? BioMed Research International. 2016;2016:2150763.
10. Tian J, An X, Niu L. Role of microRNAs in cardiac development and disease. Experimental and therapeutic medicine. 2017;13(1):3-8.
11. Mitchelson KR, Qin W-Y. Roles of the canonical myomiRs miR-1,-133 and-206 in cell development and disease. World journal of biological chemistry. 2015;6(3):162.
12. Oliveira-Carvalho V, Carvalho VO, Bocchi EA. The emerging role of miR-208a in the heart. DNA and cell biology. 2013;32(1):8-12.
13. Tu Y, Wan L, Fan Y, Wang K, Bu L, Huang T, et al. Ischemic postconditioning-mediated miRNA-21 protects against cardiac ischemia/reperfusion injury via PTEN/Akt pathway. PLoS One. 2013;8(10):e75872.
14. Zhang D, Li Z, Wang Z, Zeng F, Xiao W, Yu A. MicroRNA-126: a promising biomarker for angiogenesis of diabetic wounds treated with negative pressure wound therapy. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy. 2019;12:1685.
15. Wu Y, Zhu B, Chen Z, Duan J, Luo A, Yang L, et al. New Insights Into the Comorbidity of Coronary Heart Disease and Depression. Current problems in cardiology. 2019, 12:100413.
16. Vegter EL, van der Meer P, de Windt LJ, Pinto YM, Voors A. MicroRNAs in heart failure: from biomarker to target for therapy. European Journal of Heart Failure. 2016;18(5):457-68.
17. Sun T, Dong Y-H, Du W, Shi C-Y, Wang K, Tariq M-A, et al. The role of microRNAs in myocardial infarction: from molecular mechanism to clinical application. International Journal of Molecular Sciences. 2017;18(4):745.
18. Hosseini A, Dolati S, Hashemi V, Abdollahpour‐Alitappeh M, Yousefi M. Regulatory T and T helper 17 cells: Their roles in preeclampsia. Journal of Cellular Physiology. 2018;233(9):6561-73.
19. Yang B, Lin H, Xiao J, Lu Y, Luo X, Li B, et al. The muscle-specific microRNA miR-1 regulates cardiac arrhythmogenic potential by targeting GJA1 and KCNJ2. Nature Medicine. 2007;13(4):486.
20. Widmer RJ, Lerman LO, Lerman A. MicroRNAs: small molecule, big potential for coronary artery disease. Oxford University Press; 2016.
21. Fichtlscherer S, De Rosa S, Fox H, Schwietz T, Fischer A, Liebetrau C, et al. Circulating microRNAs in patients with coronary artery diseasenovelty and significance. Circulating Research. 2010;107(5):677-84.
22. Dolati S, Aghebati‐Maleki L, Ahmadi M, Marofi F, Babaloo Z, Ayramloo H, et al. Nanocurcumin restores aberrant miRNA expression profile in multiple sclerosis, randomized, double‐blind, placebo‐controlled trial. Journal of Cellular Physiology. 2018;233(7):5222-30.
23. Su Q, Yang H, Li L. Circulating miRNA-155 as a Potential Biomarker for Coronary Slow Flow. Disease Marker. 2018;2018.
24. Zhang Y, Li H-H, Yang R, Yang B-J, Gao Z-Y. Association between circulating microRNA-208a and severity of coronary heart disease. Scandinavian Journal of Clinical and Laboratory Investigation. 2017;77(5):379-84.
25. Limana F, Esposito G, D'Arcangelo D, Di Carlo A, Romani S, Melillo G, et al. HMGB1 attenuates cardiac remodelling in the failing heart via enhanced cardiac regeneration and miR-206-mediated inhibition of TIMP-3. Plos One. 2011;6(6):e19845.
26. Wang M, Ji Y, Cai S, Ding W. MiR-206 suppresses the progression of coronary artery disease by modulating vascular endothelial growth factor (VEGF) expression. Medical Science Monitor. 2016;22:5011.
27. Fiedler J, Thum T. New Insights Into miR-17–92 Cluster Regulation and Angiogenesis. Am Heart Assoc; 2016.
28. Vegter EL, Ovchinnikova ES, van Veldhuisen DJ, Jaarsma T, Berezikov E, van der Meer P, et al. Low circulating microRNA levels in heart failure patients are associated with atherosclerotic disease and cardiovascular-related rehospitalizations. Clinical Research in Cardiology. 2017;106(8):598-609.
29. Diehl P, Fricke A, Sander L, Stamm J, Bassler N, Htun N, et al. Microparticles: major transport vehicles for distinct microRNAs in circulation. Cardiovascular Research. 2012;93(4):633-44.
30. Guo J-F, Zhang Y, Zheng Q-X, Zhang Y, Zhou H-H, Cui L-M, et al. Association between elevated plasma microRNA-223 content and severity of coronary heart disease. Scandinavian Journal of Clinical and Laboratory Investigation 2018:1-6.
31. Jiang Y, Liu N, Xue B, Hou J, Lin C, Ren J, et al. Circulating MicroRNA Profiles Differ between Hyperglycemia and Euglycemia in Coronary Heart Disease Patients. BioMed Research International. 2017;2017:9192575.
32. Cao Z, Qian L, Wu S. Roles of miR-16 in vascular endothelial injury in patients with coronary heart disease. Biomedical Research. 2017;28(5).
33. Hoekstra M, van der Lans CA, Halvorsen B, Gullestad L, Kuiper J, Aukrust P, et al. The peripheral blood mononuclear cell microRNA signature of coronary artery disease. Biochemical and Biophysical Research Communications. 2010;394(3):792-7.
34. Dolati S, Ahmadi M, Aghebti-Maleki L, Nikmaram A, Marofi F, Rikhtegar R, et al. Nanocurcumin is a potential novel therapy for multiple sclerosis by influencing inflammatory mediators. Pharmacological Reports. 2018;70(6):1158-67.
35. Bernhagen J. Protective cardiac conditioning by an atypical cytokine. Clinical Science. 2019;133(8):933-7.
36. Dolati S, Marofi F, Babaloo Z, Aghebati-Maleki L, Roshangar L, Ahmadi M, et al. Dysregulated Network of miRNAs Involved in the Pathogenesis of Multiple Sclerosis. Biomedicine Pharmacotherapy. 2018;104:280-90.
37. Renjie W, Haiqian L. MiR-132, miR-15a and miR-16 synergistically inhibit pituitary tumor cell proliferation, invasion and migration by targeting Sox5. Cancer letters. 2015;356(2):568-78.
38. Montoya MM, Maul J, Singh PB, Pua HH, Dahlström F, Wu N, et al. A distinct inhibitory function for miR-18a in Th17 cell differentiation. The Journal of Immunology. 2017;199(2):559-69.
| Files | ||
| Issue | Vol 19, No 1 (2020) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijaai.v19i1.2418 | |
| PMID | 32245321 | |
| Keywords | ||
| Cytokines Inflammation miRNA Slow coronary flow | ||
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How to Cite
1.
Danaii S, Shiri S, Dolati S, Ahmadi M, Ghahremani-Nasab L, Amiri A, Kamrani A, Samadi Kafil H, Chakari-Khiavi F, Hojjat-Farsangi M, Malek Mahdavi A, Mehdizadeh A, Yousefi M. The Association between Inflammatory Cytokines and miRNAs with Slow Coronary Flow Phenomenon. Iran J Allergy Asthma Immunol. 2020;19(1):56-64.
