Original Article
  

Effect of Influenza Virus Infection in a Murine Model of Asthma

Abstract

Respiratory virus infection is a major cause of asthma exacerbation. However, the underlying mechanisms of  this exacerbation  are unknown. Therefore,  to  determine  the mechanisms, we examined the effect of influenza infection in a murine model of asthma.Mice were divided into four groups: the phosphate-buffered saline (PBS), house dust mite (HDM), influenza, and HDM/influenza groups. The influenza group and the HDM/influenza group were infected with influenza A virus. We measured airway resistance (Penh value), examined the lung tissue for pathology, and analyzed the cells and cytokines in bronchoalveolar lavage fluid (BALF) by ELISA.At 50 mg/mL methacholine, the HDM/influenza group showed a significantly higher Penh value than the PBS, HDM, and influenza groups. The number of neutrophils in BALF was higher in the HDM/influenza group than in the HDM group. A significantly greater number of lymphocytes and macrophages were detected in the HDM/influenza group than in the HDM group. IFN-γ and IL-1β levels were higher in the HDM/influenza group than in the HDM group. IL-5 levels did not vary between the HDM and HDM/influenza groups, IL-10 was significantly lower in the HDM/influenza than in the HDM group. Chemokine (C-X-C motif) ligand 1 (CXCL1) and regulated upon activation, normal T cell expressed and secreted (RANTES) were higher in the HDM/influenza group than in the HDM group.In a murine model of asthma, influenza-induced airway inflammation appeared to becaused by simultaneous activation of neutrophilic and eosinophilic inflammation.

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IssueVol 14, No 4 (2015) QRcode
SectionOriginal Article(s)
Keywords
Asthma Cytokine Eosinophil Histology Influenza Mouse Neutrophil
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How to Cite
1.
Kim H, Lee H, Kim H, Won S, Lee E, Bang K, Chun Y, Yoon J, Kim H, Kim JT. Effect of Influenza Virus Infection in a Murine Model of Asthma. Iran J Allergy Asthma Immunol. 2015;14(4):392-401.