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The Main Relaxant Constituents of Nigella Sativa Methanolic Fraction on Guinea Pig Tracheal Chains

Abstract

Our  previous  studies demonstrated  the  preventive effect  of  Nigella  sativa  extract  on asthma and water-soluble substances of this extract, especially its methanol fraction were responsible for this relaxation on contracted tracheal chains of guinea pigs. For the first time, the present study has been conducted to determine the main constituents of its methanolic extract.
Four constituents of 20%-methanolic fraction, consisting of two flavonoids (20-20% and 21-20% fractions) and two polysaccharides (1-20% and 2-20% fractions), were purified by analytical and  preparative  HPLC.  The  relaxant  effects  of  four  cumulative  concentrations  of  each constituent (50, 100, 150 and 200 mg/lit) in comparison with saline (1 ml) as negative control and four cumulative concentrations of theophylline (0.2, 0.4, 0.6 and 0.8 mM) as positive control were examined on methacholine-precontracted guinea pig tracheal chains (n=6).
All concentrations  of theophylline and most concentrations  of 20-20, 21-20 fractions showed significant relaxant effects compared to  that  of saline. 20-20 fraction (Comferol diglucoside) was the most potent bronchodilator. Their relaxant effects were lower than that of theophylline. Polysaccharides (1-20, 2-20 fractions) did not  have any relaxant effects compared to that of saline.
These results revealed that two flavonoids of 20%-methanolic fraction of Nigella  sativa were its main relaxant constituents.

1. Bousquet J, Mantzouranis E, Cruz AA, Aït-Khaled N, Baena-Cagnani CE, Bleecker ER, et al. Uniform definition of asthma severity, control, and exacerbations: Document presented for the World Health Organization Consultation on severe asthma. J Allergy Clin Immunol 2010; 126(5):926-38.
2. Goreja WG. In: Black seed: Nature’s miracle remedy.New York, NY: Amazing herbs press Ltd, 2003: 5-10.
3. El-Dakhakhny M. Studies on chemical constitution of Egyptian Nigella sativa L.seeds. II. The essential oil. Planta Medica 1963; 11:465-70.
4. Atta UR, Malik SO. Nigellidine, a new indazol alkaloid from seeds of Nigella sativa. J Res Inst 1995; 36:1993-6.
5. Atta UR, Malik SO. Nigellimine N-oxide, a new isoquinoline alkaloid from the seeds of Nigella sativa. Hetrocycles 1985; 23:953-5.
6. Ansari AK, Sadiy HAS. Structural studies on a saponin isolated from the seeds of Nigella sativa. Phyto Chem 1989; 27:377-9.
7. Avicenna: Law in Medicine, Interpreter; Sharafkhandy A, Tehran: Ministry of Guidance publication, 1990: 314.
8. Boskabady MH, Kiani S, Jandaghi P, Ziaei T, Zarei A.Antitussive of Nigella sativa. Pak J Med Sci 2004;20(3):224-8.
9. Ali BH, Blunden G. Pharmacological and toxicological properties of Nigella sativa. Phytother Res 2003;17(4);299-305.
10. Labib Salem M. Immunomodulatory and therapeutic properties of the Nigella sativa L. seed. Int immunopharmacol 2005; 5(13-14):1749-70.
11. Aqel MB.The relaxing effect of volatile oil of Nigella sativa seed on vascular smooth muscle. Jordan Ser B 1992; 1:91-100.
12. Aqel MB. Effects of Nigella sativa seeds on intestinal smooth muscle. Int J Pharmacogn 1993; 31:55-60.
13. Reiter M, Brandt W. Relaxant effects on tracheal and ileal smooth muscles of the guinea- pig. Arzneimittelforschung 1985; 35(1A):408-14.
14. Keyhanmanesh R, Boskabady MH, Ebrahimi Saadatloo MA. The contribution of water and lipid soluble substances in the relaxant effects of Nigella sativa extract on guinea pig tracheal smooth muscle (in vitro). IJBMS 2007; 10(3):154–61.
15. Boskabady MH, Keyhanmanesh R, Ebrahimi Saadatloo MA. Relaxant effects of different fractions from Nigella sativa L. on guinea pig tracheal chains and its possible mechanism(s). Indian J of Exp Biol 2008; 46(12):805-10.
16. Boskabady MH, keyhanmanesh R, Khamneh S, Doostar Y, Khakzad MR. Potential immunomodulation effect of the extract of Nigella sativa on ovalbumin sensitized guinea pigs. J Zhejiang Univ Sci B 2011; 12(3):201-9.
17. Boskabady MH, Keyhanmanesh R, Khamneh S, Ebrahimi MA. The effect of Nigella sativa extract on tracheal responsiveness and lung inflammation in ovalbumin sensitized guinea pigs. Clinics (Sao Paulo) 2011;66(5):879-87.
18. Keyhanmanesh R, Bagban H, Nazemiyeh H, Mirzaei Bavil F, Alipour MR, Ahmady M. The Relaxant Effects of Different Methanolic Fractions of Nigella sativa on Guinea Pig Tracheal Chains. IJBMS 2013; 16:123-8.
19. Carron R, Sanz E, Puebla P, Martin ML, Roman LS,Guerrero MF. Mechanisms of relaxation induced by flavonoid ayanin in isolated aorta rings from Wistar rats. Colomb Med 2010; 41(1):10-6.
20. Ajaya M, Gilani AH, MR. Effects of flavonoids on vascular smooth muscle of the isolated rat thoracic aorta. Life Sci 2003; 74(5):603–12.
21. Woodman OL, Chan ECH. Vascular and anti-oxidant actions of flavonols and flavones. Clinic Experiment Pharmacol Physiol 2004; 31(11):786-90.
22. Ko WC, Liu PY, Chen JL, Leu IJ, Shih CM. Relaxant Effects of Flavonoids in Isolated Guinea Pig Trachea and Their Structure-Activity Relationships. Planta Med 2003; 69(12):1086-90.
23. Ajay M, Gilani AU, Mustafa MR. Effects of flavonoids on vascular smooth muscle of the isolated rat thoracic aorta. Life Sci 2003; 74(5):603-12.
24. Macedo CL, Vasconcelos LHC, Correia ACC, Martins IRR, Lira DP, Santos BVO, et al. Spasmolytic effect of galetin 3, 6-dimethyl ether, a flavonoid obtained from Piptadenia stipulacea (Beth) Ducke. J smooth muscle Res 2011; 47(5):123-34.
25. Essayan DM. Cyclic nucleotide phosphodiesterases. J Allergy Clin Immunol 2001; 108(5):671–80.
26. Deree J, Martins JO, Melbostad H, Loomis WH, Coimbra R. Insights into the Regulation of TNF-α Production in Human Mononuclear Cells: The Effects of Non-Specific Phosphodiesterase Inhibition. Clinics (Sao Paulo) 2008; 63(3):321–8.
27. Peters-Golden M, Canetti C, Mancuso P, Coffey MJ. Leukotrienes: underappreciated mediators of innate immune responses. J Immunol 2005; 174(2):589–94.
28. Boskabady MH, Shirmohammadi B, Jandaghi P, Kiani S.Possible mechanisms for relaxant effect of aqueous and macerate extracts from Nigella sativa on tracheal chains of guinea pig. BMC Pharmacol 2004; 4:3.

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Keywords
Guinea pig Main constituent Methanolic fraction Nigella sativa Relaxant effect
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1.
Keyhanmanesh R, Bagban H, Nazemieh H, Mirzaei Bavil F, Alipour MR. The Main Relaxant Constituents of Nigella Sativa Methanolic Fraction on Guinea Pig Tracheal Chains. Iran J Allergy Asthma Immunol. 1;12(2):136-143.