The Effects of Vitamin D on Allergen-Induced Expression of Interleukin-13 and Interleukin-17 in Cord Blood CD4+T Cells
Abstract
Cytokine production in response to allergens may influence the development of atopy- predisposing immune responses, initializing the early programming of allergy and asthma. Vitamin D intake may be protective due to its immunoregulatory properties, that may contribute to influence the expression of the atopic phenotype initiated in early life. The objective of our study was to investigate the effects of 1,25-(OH)2D3 on allergen-stimulated expression of asthma related cytokines in cord blood T cells.
Cord blood samples were collected from the umblilical vein of 24 term deliveries during labor, CD4+T cells derived from cord blood mononuclear cells (CBMCs), were cultured for 72 hours with ovalbumin (OVA), β-lactoglobulin (β-LG), respectively, in presence or absence of 1,25-(OH)2D3 to detect the levels of interleukin-13 (IL-13) and interleukin-17 (IL-17) in culture supernatants and the mRNA expressions in CD4+T cells.
After allergens stimulation, CD4+T cells showed an increase of IL-13 and IL-17 production, while cultured in the presence of 1,25-(OH)2D3 displayed a statistically significant down-regulation of allergen-induced expression of IL-13 and IL-17 in CD4+T cells.
These results indicated that allergens may induce changes in CD4+T cell function to increase inflammatory cytokine production. 1,25-(OH)2D3 modulated the capacity of CD4+T cells in response to allergens, which might be protective for allergy.
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| Issue | Vol 13, No 2 (2014) | |
| Section | Articles | |
| Keywords | ||
| 1 25-(OH)2D3 Allergens CD4-Positive T-Lymphocytes Interleukin-13 Interleukin-17 | ||
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