Mannan-Binding Lectin Serum Levels in 593 Healthy Iranian Children and Adults
Mannan-binding lectin (MBL) is a vital protein of innate immune system and has two critical functions: complement activation through the lectin pathway and opsonization. MBL deficiency has been classified as the most common inherited immunodeficiency known in humans (about 30% of the population), and is associated with predisposition to infections and high risk of some autoimmune diseases. The purpose of this study was to determine the profile of MBL serum level in Iranian healthy population in association with sex and age groups for the first time. We studied the serum concentration of MBL in 593 Iranian healthy cases: 340 males and 235 females in 4 different age groups by using enzyme-linked immunosorbent assay. The mean serum levels of MBL were 3.854 ± 2.77 µg/ml at the age of less than 6 months, 4.147 ± 3.54 µg/ml at 6 months to 2 years of age, 4.410 ± 3.09 µg/ml at 2-6 years and 2.207 ± 1.73 µg/ml in adults. There was significant differences in the mean concentration of MBL among different age groups of children and also between children and adults (p<0.05). No association was observed between sex and MBL concentrations. MBL serum levels of Iranian population seem to be different from some of other populations which may be explained by genetic variations. The MBL values in this study can be used as a normal reference range for future studies in Iranian population.
1. Aittoniemi J, Baer M, Soppi E, Vesikari T, Miettinen A.Mannan binding lectin deficiency and concomitant immunodefects. Arch Dis Child 1998; 78(3):245-8.
2. Lee SG, Yum JS, Moon HM, Kim HJ, Yang YJ, Kim HL, et al. Analysis of mannose-binding lectin 2 (MBL-2) genotype and the serum protein levels in the Korean population. Mol Immunol 2005; 42(8):969-77.
3. Sullivan K, Winkelstein J.deficiency of the complement system. In: Stiehm ER, Ochs HD, Winkelstein JA. Immunologic disorders in infants and children. 5th ed. Philadelphia: Elsevier Saunders, 2004:652-84.
4. Minchinton R, Dean M, Clark T, Heatley S, Mullighan CG. Analysis of the relationship between Mannose- Binding lectin (MBL) Genotype, MBL levels and functional in an Australian blood donor Population. Scand J Immunol 2002; 56(6):630-41.
5. Trevisiol SC, Boniotto M, Giglio L, Poli F, Morgutti M, Crovella S. MBL2 polymorphism screening in a regional Italian CF center. J Cyst Fibros 2005; 4(3):189-91.
6. Turner MW, Dinan L, Heatley S, Jack DL, Boettcher B, Lester S, et l. Restricted polymorphism of the mannose- binding lectin gene of indigenous Australians. Hum Mol Genet 2000; 9(10):1481–6.
7. Bouwman LH, Roep BO, Roos A. Mannose-binding lectin: clinical implications for infection, transplantation, and autoimmunity. Hum Immunol 2006; 67(4–5):247–56.
8. Summerfield JA. Clinical potential of mannose-binding lectin-replacement therapy. Biochem Soc Trans 2003;31(Pt 4):770-3.
9. Unsworth,DJ. complement deficiency and disease. J.clin Pathol 2008; 61(9):1013-7.
10. Gupta K, Gupta RK, Hajela K. Disease associations of mannose-binding lectin & potential of replacement therapy. Indian J Med Res 2008; 127(5):431-40.
11. Aittoniemi J, Miettinen A, Laippala P, Isolauri E, Viikari J, Ruuska T , et al. Age-dependent variation in the serum concentration mannan- binding protein . Acta Paediatr 1996; 85(8):906-9.
12. Chen J, Xu Z, Ou X, Wang M, Yang X, Li Q. Mannose-binding Lectin polymorphisms and recurrent respiratory tract infection in Chinese children. Eur J Pediatr 2009;168(11):1305-13.
13. Kilpatrick D. therapeutic applications of mannan-binding lectin. Biochem Soc Trans 2003; 31(pt4):745-7.
14. Heitzeneder S, Seidel M, Forster-Waldl E, Heitger A.Mannan-binding lectin deficiency _ Good news, bad news, doesn’t matter?. Clin Immunl 2012; 143(1):22-38.
15. Terai I, Kobayashi K, Fujita T, Hagiwara K. Human Serum Mannose Binding Protein (MBP): Development of an Enzyme-Linked Immunosorbent Assay (ELISA) and Determination of Levels in Serum from 1085 Normal Japanese and in Some Body Fluids. Biochem Med Metab Biol 1993; 50(1):111–19.
16. Sallenbach S, Thiel S, Aebi C, Otth M, Bigler S,Jensenius JC, et al. Serum concentrations of lectin- pathway components in healthy neonates, childrens and adults: mannan-binding lectin (MBL), M-, L-, and H- ficolin, and MBL-associated serine protease-2 (MASP-2). Pediatr Allergy Immunol 2011; 22(4):424–30.
|Issue||Vol 13, No 2 (2014)|
|Iran Mannan-Binding Lectin Reference value|
|Rights and permissions|
|This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.|