Evaluation of The Correlation and Reproducibility between Histamine, IL-4, and IL-13 Release from Human Basophils
,
Abstract
Human basophils play a key role in allergic diseases such as asthma and in a variety of immunological disorders. The generation of IL-4 and IL-13 can be induced from basophil by IgE-mediated and non-IgE-mediated mechanisms. Time and stimulus- dependent differences in the regulation of these cytokines could have relevance to their biological effects. The aim of the present study was activation of basophils in order to evaluate the extent of histamine, IL-4, and IL-13 generations.
Basophil-enriched suspensions were prepared by Percoll gradients. The release of histamine and cytokines was assessed after activation with either anti-human IgE (1/1000 or1/10000, 4 h or 24 h) or IL-3 (100 ng/ ml, 24 h). Results were analysed statistically, using ANOVA test.
Using anti-IgE, there was no significant correlation between the extent of either IL-4 (r=0.24, p=0.35) or IL-13 (r=0.47, p=0.098) and histamine release. Using IL-3 as stimulator, results showed that the extent of IL-13 correlated with histamine release(r=0.44, p=0.036). There was no correlation between the extent of IL-4 and the degree of either histamine (r=0.077, p=0.72) or IL-13 (r=0.162, p=0.5). The reproducibility of cytokines isolated from the same donor (on different occasions) indicated that the ability of anti-IgE to induce cytokines was consistently similar for a given donor.
Our data showed that the pathways leading to IL-3-triggering histamine release and IL-13 generation show similarity. Donor-dependent differences may be responsible for this wide range in the extent of releasibility. The ability of IL-3 to release cytokines from basophils showed a wider range.
1. Warner JA, Kroegel C. Pulmonary immune cells in health and disease: mast cells and basophils. Eur Respir J 1994;7(7):1326-41.
2. Bochner BS, Busse WW. Allergy and asthma. J Allergy Clin Immunol 2005;115(5):953-9.
3. Dvorak HF, Dvorak AM. Basophilic leucocytes:structure, function and role in disease. Clin Haematol 1975; 4(3):651-83.
4. Schleimer RP, MacGlashan DW, Jr., Peters SP, Naclerio R, Proud D, Adkinson NF, Jr., et al. Inflammatory mediators and mechanisms of release from purified human basophils and mast cells. J Allergy Clin Immunol 1984; 74(4 Pt 1):473-81.
5. Schroeder JT, Kagey-Sobotka A, Lichtenstein LM. The role of the basophil in allergic inflammation. Allergy 1995; 50(6):463-72.
6. Schroeder JT, MacGlashan DW, Jr., Kagey-Sobotka A, White JM, Lichtenstein LM. Cytokine generation by human basophils. J Allergy Clin Immunol. 1994; 94(6 Pt2):1189-95.
7. Brunner T, Heusser CH, Dahinden CA. Human peripheral blood basophils primed by interleukin 3 (IL-3) produce IL-4 in response to immunoglobulin E receptor stimulation. J Exp Med 1993; 177(3):605-11.
8. Nahum LH. Allergy, Histamine, and the Basophil. ConnMed 1963; 27:367-71.
9. Skov-Stahl P, Norh S, Weeke B. Basophil histamine release in patients with hay fever. Results compared with specific IgE and total IgE during immunotherapy. Clin Exp Immunol 1977; 27(3):432-9.
10. Macfarlane AJ, Kon OM, Smith SJ, Zeibecoglou K, Khan LN, Barata LT, et al. Basophils, eosinophils, and mast cells in atopic and nonatopic asthma and in late-phase allergic reactions in the lung and skin. J Allergy Clin Immunol 2000; 105(1 Pt 1):99-107.
11. Barnes PJ. Cytokines as mediators of chronic asthma. AmJ Respir Crit Care Med 1994; 150(5 Pt 2):S42-9.
12. MacGlashan D, Jr., White JM, Huang SK, Ono SJ, Schroeder JT, Lichtenstein LM. Secretion of IL-4 from human basophils. The relationship between IL-4 mRNA and protein in resting and stimulated basophils. J Immunol 1994; 152(6):3006-16.
13. Gibbs BF, Haas H, Falcone FH, Albrecht C, Vollrath IB, Noll T, et al. Purified human peripheral blood basophils release interleukin-13 and preformed interleukin-4 following immunological activation. Eur J Immunol 1996; 26(10):2493-8.
14. Shimizu Y, Shichijo M, Hiramatsu K, Takeuchi M, Nagai H, Takagi K. Mite antigen-induced IL-4 and IL-13 production by basophils derived from atopic asthma patients. Clin Exp Allergy 1998; 28(4):497-503.
15. Falcone FH, Haas H, Gibbs BF. The human basophil: a new appreciation of its role in immune responses. Blood 2000; 96(13):4028-38.
16. Jung T, Wijdenes J, Neumann C, de Vries JE, Yssel H.Interleukin-13 is produced by activated human CD45RA+ and CD45RO+ T cells: modulation by interleukin-4 and interleukin-12. Eur J Immunol 1996; 26(3):571-7.
17. Kon OM, Sihra BS, Till SJ, Corrigan CJ, Kay AB, Grant JA. Unstimulated basophils in atopic and nonatopic subjects express intracellular interleukin-4: detection by flow cytometry. Allergy 1998; 53(9):891-6.
18. Li H, Sim TC, Alam R. IL-13 released by and localized in human basophils. J Immunol 1996; 156(12):4833-8.
19. Schroeder JT, Howard BP, Jenkens MK, Kagey-Sobotka A, Lichtenstein LM, MacGlashan DW Jr. IL-4 secretion and histamine release by human basophils are differentially regulated by protein kinase C activation. J Leukoc Biol 1998; 63(6):692-8.
20. Kraan MC, Versendaal H, Jonker M, Bresnihan B, Post WJ, t Hart BA, et al. Asymptomatic synovitis precedes clinically manifest arthritis. Arthritis Rheum 1998;41(8):1481-8.
21. Obiri NI, Leland P, Murata T, Debinski W, Puri RK. The IL-13 receptor structure differs on various cell types and may share more than one component with IL-4 receptor. J Immunol 1997; 158(2):756-64.
22. Sin AZ, Roche EM, Togias A, Lichtenstein LM, Schroeder JT. Nerve growth factor or IL-3 induces more IL-13 production from basophils of allergic subjects than from basophils of nonallergic subjects. J Allergy Clin Immunol 2001; 108(3):387-93.
23. Tedeschi A, Salmaso C, Di Donato M, Lorini M, Miadonna A. Granulocyte-macrophage colony- stimulating factor and interleukin-3 cause basophil histamine release by a common pathway: downregulation by sodium. Immunology 1999; 96(2):164-70.
24. Tedeschi A, Palella M, Milazzo N, Lorini M, Miadonna A. IL-3-induced histamine release from human basophils. Dissociation from cationic dye binding and down- regulation by Na+ and K+. J Immunol 1995;155(5):2652-60.
25. Miura K, Saini SS, Gauvreau G, MacGlashan DW, Jr.Differences in functional consequences and signal transduction induced by IL-3, IL-5, and nerve growth factor in human basophils. J Immunol 2001; 167(4):2282-91.
26. Ochensberger B, Tassera L, Bifrare D, Rihs S, Dahinden CA. Regulation of cytokine expression and leukotriene formation in human basophils by growth factors, chemokines and chemotactic agonists. Eur J Immunol 1999; 29(1):11-22.
27. Zheng X, Karsan A, Duronio V, Chu F, Walker DC, Bai TR, et al. Interleukin-3, but not granulocyte-macrophage colony-stimulating factor and interleukin-5, inhibits apoptosis of human basophils through phosphatidylinositol 3-kinase: requirement of NF- kappaB-dependent and -independent pathways. Immunology 2002; 107(3):306-15.
28. Valent P, Schmidt G, Besemer J, Mayer P, Zenke G, Liehl E, et al. Interleukin-3 is a differentiation factor for human basophils. Blood 1989; 73(7):1763-9.
29. Hirai K, Morita Y, Misaki Y, Ohta K, Takaishi T, Suzuki S, et al. Modulation of human basophil histamine release by hemopoietic growth factors. J Immunol 1988;141(11):3958-64.
30. Schleimer RP, Derse CP, Friedman B, Gillis S, Plaut M, Lichtenstein LM, et al. Regulation of human basophil mediator release by cytokines. I. Interaction with antiinflammatory steroids. J Immunol 1989; 143(4):1310-7.
31. Redrup AC, Howard BP, MacGlashan DW, Jr., Kagey- Sobotka A, Lichtenstein LM, Schroeder JT. Differential regulation of IL-4 and IL-13 secretion by human basophils: their relationship to histamine release in mixed leukocyte cultures. J Immunol 1998; 160(4):1957-64.
32. Ennis MP, Wisdom GB. A magnetizable solid phase for enzyme extraction. Appl Biochem Biotechnol 1991;30(2):155-64.
33. Siraganian RP. An automated continuous-flow system for the extraction and fluorometric analysis of histamine. Anal Biochem 1974; 57(2):383-94.
34. Gibbs BF. Human basophils as effectors and immunomodulators of allergic inflammation and innate immunity. Clin Exp Med 2005; 5(2):43-9.
35. Chen YH, Bieneman AP, Creticos PS, Chichester KL, Schroeder JT. IFN-alpha inhibits IL-3 priming of human basophil cytokine secretion but not leukotriene C4 and histamine release. J Allergy Clin Immunol 2003;112(5):944-50.
36. Miadonna A, Roncarolo MG, Lorini M, Tedeschi A.Inducing and enhancing effects of IL-3, -5, and -6 and GM-CSF on histamine release from human basophils. Clin Immunol Immunopathol 1993; 67(3 Pt 1):210-5.
37. Numata A, Shimoda K, Kamezaki K, Haro T, Kakumitsu H, Shide K, et al. Signal transducers and activators of transcription 3 augments the transcriptional activity of CCAAT/enhancer-binding protein alpha in granulocyte colony-stimulating factor signaling pathway. J Biol Chem 2005; 280(13):12621-9.
38. Jiang X, Ng E, Yip C, Eisterer W, Chalandon Y, Stuible M, et al. Primitive interleukin 3 null hematopoietic cells transduced with BCR-ABL show accelerated loss after culture of factor-independence in vitro and leukemogenic activity in vivo. Blood 2002; 100(10):3731-40.
| Files | ||
| Issue | Vol 13, No 3 (2014) | |
| Section | Articles | |
| Keywords | ||
| Cytokine Histamine IL-4 IL-13 | ||
| Rights and permissions | |
|
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
