Articles
  

Development of a New Immunochromatographic Assay Using Gold Nanoparticles for Screening of IgA Deficiency

Abstract

A new competitive immunochromatography (ICG) strip test based on polyclonal antibody (pAb) conjugated with gold nanoparticles (NPs) was developed and its applications for primary screening of immunoglobulin (Ig) A in serum were evaluated. Nanocolloidal gold as the detection reagent, with an average particle diameter of 20 nm, was synthesized and labelled pAb. The antibody-nanocolloidal gold probe was applied on the conjugate pad, and human IgA was immobilized on a nitrocellulose membrane as the capture reagent to prepare the ICG strip test. It took only 10 minutes to accomplish a semi-quantitative detection of serum IgA in this assay. In the optimized investigational conditions, the ICG strip test could distinguish human serum IgA in the range from 1 to 270 ng/mL with a detection limit of 5 ng/mL. The reliability of testing procedures was examined by performing the ICG strip test with 11 serum samples and comparing the results with those obtained via enzyme-linked immunosorbent assay (ELISA). The ICG strip was sufficiently sensitive and accurate for a rapid screening of IgA in human serum.

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IssueVol 14, No 1 (2015) QRcode
SectionArticles
Keywords
IgA deficiency Immunoglobulin A Immunochromatography Gold nanoparticle Screening test
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How to Cite
1.
Goudarzi S, Ahmadi A, Farhadi M, Kamrava SK, Saghafi S, Omidfar K. Development of a New Immunochromatographic Assay Using Gold Nanoparticles for Screening of IgA Deficiency. Iran J Allergy Asthma Immunol. 1;14(1):105-112.