Lack of Association between Interleukin-8 Gene +781 C/T Polymorphism and Henoch–Schönlein Purpura in Childhood
-
Hui Xu
,
Yan-Xiang Pan
,
Junfeng Zhang
,
Yujie Liu
,
Jian-Hua Mao
,
Wei Li
Abstract
Henoch–Schönlein purpura (HSP), a common allergic hemorrhagic disease, occurs frequently in children affecting kidney, joint and skin. While interleukin-8 (IL-8) plays an important role in inflammation, the association between IL-8 gene +781 C/T polymorphism and HSP remains unclear. Interleukin-8, an important chemokine related to the initiation and amplification of acute inflammatory responses, has been reported to be involved in the pathogenesis of some autoimmune and inflammatory diseases. In this study, we aimed to investigate whether IL-8 gene +781 C/T (rs2227306) polymorphism has an influence on susceptibility and clinical manifestations of patients to HSP. This hospital-based case-control study comprised 192 patients with HSP and 202 healthy controls. The genotypes of IL-8 gene +781 C/T polymorphism were identified using PCR-TaqMan method. All genotype frequencies of both groups (patients and controls) conformed to the Hardy–Weinberg equilibrium. No significant differences in allele or genotype frequencies of IL-8 gene +781 C/T polymorphism were observed between patients with HSP and controls (p=0.98, χ2=0.000 and p=0.49, χ2=1.432, respectively). When patients were stratified for the presence of joint, gastrointestinal and renal manifestations, genotype frequencies of IL-8 gene polymorphism were found no statistically significant differences (p>0.05). Our findings do not support that IL-8 gene +781 C/T polymorphism has an effect on the susceptibility to HSP in Chinese children.
Saulsbury FT. Henoch-Schonlein purpura. Curr Opin Rheumatol 2001; 13(1):35-40.
2. Yang YH, Hung CF, Hsu CR, Wang LC, Chuang YH, Lin YT, et al. A nationwide survey on epidemiological characteristics of childhood Henoch-Schonlein purpura in Taiwan. Rheumatology 2005; 44(5):618-22.
3. Ballinger S. Henoch-schonlein purpura. Curr Opin Rheumatol 2003; 15(5):591-4.
4. Sano H, Izumida M, Shimizu H, Ogawa Y. Risk factors of renal involvement and significant proteinuria in Henoch- Schonlein purpura. Eur J Pediatr 2002; 161(4):196-201.
5. Chen O, Zhu XB, Ren P, Wang YB, Sun RP, Wei DE.Henoch Schonlein Purpura in children: clinical analysis of 120 cases. Afr Health Sci 2013; 13(1):94-9.
6. Amoli MM, Thomson W, Hajeer AH, Calviño MC, Garcia-Porrua C, Ollier WE, et al. Henoch-Schonlein purpura and cutaneous leukocytoclastic angiitis exhibit different HLA-DRB1 associations. J Rheumatol 2002;29(5):945-7.
7. Lopez-Mejias R, Genre F, Perez BS, Castañeda S, Ortego- Centeno N, Llorca J, et al. HLA-DRB1 association with Henoch-Schonlein purpura. Arthritis Rheumatol 2014; [Epub ahead of print]
8. Baggiolini M, Walz A, Kunkel SL. Neutrophil-activating peptide-1/interleukin 8, a novel cytokine that activates neutrophils. J Clin Invest 1989; 84(4):1045-9.
9. Yang YH, Lai HJ, Huang CM, Wang LC, Lin YT, Chiang BL. Sera from children with active Henoch-Schonlein purpura can enhance the production of interleukin 8 by human umbilical venous endothelial cells. Ann Rheum Dis 2004; 63(11):1511-3.
10. Yang YH, Huang YH, Lin YL, Wang LC, Chuang YH, Yu HH, et al. Circulating IgA from acute stage of childhood Henoch-Schonlein purpura can enhance endothelial interleukin (IL)-8 production through MEK/ERK signalling pathway. Clin Exp Immunol 2006;144(2):247-53.
11. Bosch I, Xhaja K, Estevez L, Raines G, Melichar H, Warke RV, et al. Increased production of interleukin-8 in primary human monocytes and in human epithelial and endothelial cell lines after dengue virus challenge. J Virol 2002; 76(11):5588-97.
12. Matsushima K, Oppenheim JJ. Interleukin 8 and MCAF: novel inflammatory cytokines inducible by IL 1 and TNF. Cytokine 1989; 1(1):2-13.
13. Matsushima K,Baldwin ET, Mukaida N. Interleukin-8 and MCAF: novel leukocyte recruitment and activating cytokines. Chem Immunol 1992; 51:236-65.
14. Hirani N, Antonicelli F, Strieter RM, Wiesener MS, Ratcliffe PJ, Haslett C, et al. The regulation of interleukin-8 by hypoxia in human macrophages - A potential role in the pathogenesis of the acute respiratory distress syndrome (ARDS). Mol Med 2001; 7(10):685-97.
15. Kimura S, Takeuchi S, Soma Y, Kawakami T. Raised serum levels of interleukins 6 and 8 and antiphospholipid antibodies in an adult patient with Henoch-Schonlein purpura. Clin Exp Dermatol 2013; 38(7):730-6.
16. Mukaida N, Shiroo M, Matsushima K. GENOMIC STRUCTURE OF THE HUMAN MONOCYTE- DERIVED NEUTROPHIL CHEMOTACTIC FACTOR- IL-8. J Immunol 1989; 143(4):1366-71.
17. Rovin BH, Lu L, Zhang XL. A novel interleukin-8 polymorphism is associated with severe systemic lupus erythematosus nephritis - Rapid communication. Kidney Int 2002; 62(1):261-5.
18. Gu L-q, Jia H-y, Zhao Y-j, Liu N, Wang S, Cui B, et al.Association studies of interleukin-8 gene in Graves' disease and Graves' ophthalmopathy. Endocrine 2009;36(3):452-6.
19. Emonts M, Hazes MJMW, Houwing-Duistermaat JJ, van der Gaast-de Jongh CE, de Vogel L, Han HK,et al. Polymorphisms in genes controlling inflammation and tissue repair in rheumatoid arthritis: a case control study. BMC Med Genet 2011; 12:36.
20. Suh JS, Hahn WH, Cho BS. Polymorphisms of CXCL8 and Its Receptor CXCR2 Contribute to the Development and Progression of Childhood IgA Nephropathy. J Interferon Cytokine Res 2011; 31(3):309-15.
21. Amoli MM, Thomson W, Hajeer AH, Calviño MC, Garcia-Porrua C, Ollier WE, et al. Interleukin 8 gene polymorphism is associated with increased risk of nephritis in cutaneous vasculitis. J Rheumatol 2002;29(11):2367-70.
22. Heinzmann A, Ahlert I, Kurz T, Berner R, Deichmann KA.et al. Association study suggests opposite effects of polymorphisms within IL8 on bronchial asthma and respiratory syncytial virus bronchiolitis. J Allergy Clin Immunol 2004; 114(3):671-6.
23. Hull J, Thomson A, Kwiatkowski D. Association of respiratory syncytial virus bronchiolitis with the interleukin 8 gene region in UK families. Thorax 2000;55(12):1023-7.
24. Puthothu B, Krueger M, Heinze J, Forster J, Heinzmann A. Impact of IL8 and IL8-receptor alpha polymorphisms on the genetics of bronchial asthma and severe RSV infections. Clin Mol Allergy 2006; 4:2.
25. Hildebrand F, Stuhrmann M, van Griensven M, Meier S, Hasenkamp S, Krettek C, et al. Association of IL-8-
251A/T polymorphism with incidence of Acute Respiratory Distress Syndrome (ARDS) and IL-8 synthesis after multiple trauma. Cytokine 2007;37(3):192-9.
26. He Y, Liang X, Wu X, Meng C, Wu B, Fu D, et al.Association between interleukin 8 -251 A/T and +781
C/T polymorphisms and osteoarthritis risk. Immunol Lett2014; 162(1 Pt A):207-11.
27. Sippert EA, de Oliveira e Silva C, Laguila Visentainer JE, Sell AM. Association of Duffy Blood Group Gene Polymorphisms with IL8 Gene in Chronic Periodontitis. Plos One 2013; 8(12):e83286.
28. Zhang N, Xu Y, Zhang B, Zhang T, Yang H, Zhang B, et al. Analysis of Interleukin-8 Gene Variants Reveals Their Relative Importance as Genetic Susceptibility Factors for Chronic Periodontitis in the Han Population. Plos One 2014; 9(8):e104436.
29. Hacking D, Knight JC, Rockett K, Brown H, Frampton J, Kwiatkowski DP, et al. Increased in vivo transcription of an IL-8 haplotype associated with respiratory syncytial virus disease-susceptibility. Genes Immun 2004;5(4):274-82.
30. Wacharasint P, Nakada T-a, Boyd JH, Russell JA, Walley KR. AA genotype of IL-8-251A/T is associated with low PaO2/FiO(2) in critically ill patients and with increased IL-8 expression. Respirology 2012; 17(8):1253-60.
31. Tabel Y, Mir S, Berdeli A. Interleukin 8 gene 2767 A/G polymorphism is associated with increased risk of nephritis in children with Henoch-Schonlein purpura. Rheumatol Int 2012; 32(4):941-7.
32. Mills JA, Michel BA, Bloch DA, et al. THE AMERICAN-COLLEGE-OF-RHEUMATOLOGY 1990 CRITERIA FOR THE CLASSIFICATION OF HENOCH-SCHONLEIN PURPURA. Arthritis Rheum 1990; 33(8):1114-21.
33. Schwartz GJ, Muñoz A, Schneider MF, Mak RH, Kaskel F, Warady BA, et al. New equations to estimate GFR in children with CKD. J Am Soc Nephrol 2009;20(3):629-37.
34. Seet WT,Mary Anne TJ, Yen TS. Apolipoprotein E genotyping in the Malay, Chinese and Indian ethnic groups in Malaysia-a study on the distribution of the different apoE alleles and genotypes. Clin Chim Acta 2004; 340(1-2):201-5.
35. Hampe J, Wollstein A, Lu T, Frevel HJ, Will M, Manaster C, et al. An integrated system for high throughput TaqMan based SNP genotyping. Bioinformatics 2001; 17(7):654-5.
36. Poli V. The role of C/EBP isoforms in the control of inflammatory and native immunity functions. J Biol Chem 1998; 273(45):29279-82.
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| Issue | Vol 15, No 3 (2016) | |
| Section | Original Article(s) | |
| Keywords | ||
| Case control study Henoch Schönlein purpura Inflammatory Interleukin8 Polymorphism | ||
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