Effect of Loaded Glycyrrhizic Acid on PLGA Nano-particle on Treatment of Allergic Asthma
Abstract
Asthma is considered a complex disease of the respiratory system that is characterized by bronchoconstriction, airway inflammation, cough, dyspnea, and wheezing. Allergic reactions are the main reason behind asthma which is known as an important health problem with a high rate of morbidity and mortality in patients with respiratory diseases. Liquorice, the root of Glycyrrhiza, is primarily effective for asthma which is widely used in herbal medicine. In the present study, we designed nano-particles that carry Glycyrrhizic acid as the effective component of Liquorice.
After Poly (D,L-lactide-co-glycolic acid) PLGA nanoparticle preparation and Glycyrrhizic acid loading, the morphology of the nanoparticle, the electric charge distribution, and drug-releasing ability were studied. Then the effect of Glycyrrhizic acid-PLGA on the animal model of allergic asthma was investigated.
Glycyrrhizic acid-nanoparticle had a mean±SD size of 350±50 nm. about 67% of the effective component was released after 10 h. The interleukin (IL)-4, IL-5, IL-13, and IL-25 levels and the Muc5ac mRNA expression were decreased in the Glycyrrhizic acid-PLGA treated group. In addition, a significant decline was observed in goblet cell hyperplasia, mucus hyper-secretion, and eosinophilic inflammation around bronchi and vessels of the nano-drug treated group, compared with the asthmatic group.
We found that Glycyrrhizic acid-PLGA nanoparticle had an anti-asthma effect which may be used as a new drug to cure asthma. It can prevent bronchial obstruction, breathlessness, and asthma attacks.
2. Athari SS. Targeting cell signaling in allergic asthma. Signal Transduct Target Ther.2019;4(5):45-9.
3. MehrabiNasab E, Athari SM, Motlagh B, Athari SS. Effects of oral administration of Ocimumbasilicum on goblet cell hyperplasia and upstream cytokine gene expression in allergic asthma. Rev Fr Allergol. 2020;60(2):64-8.
4. Athari SM, MehrabiNasab E, Athari SS. Study effect of Ocimumbasilicum seeds on mucus production and cytokine gene expression in allergic asthma mice model. Rev Fr Allergol. 2018;58(7):489-93.
5. Ch Ma, Ma Z, Liao EL, Liu J, Fu Q, Ma S. Immunoregulatory effects of Glycyrrhizic acid exerts anti-asthmatic effects via modulation of Th1/Th2 cytokines and enhancement of CD4+CD25+Foxp3+ regulatory T cells in ovalbumin-sensitized mice. J Ethnopharmacol. 2013;148(3):755–62.
6. Li XM. Traditional Chinese herbal remedies for asthma and food allergy. J Allergy Clin Immunol.2007;120(1):25–31.
7. Wang X, Zhang H, Chen L, Shan L, Fan G, Gao X. Liquorice, aunique “guide drug” of traditional Chinese medicine: A review of its role in drug interactions. J Ethnopharmacol. 2013;150(3):781-90.
8. Ram A, Mabalirajan U, Das M, Bhattacharya I, Dinda AK, Gangal SV, et al. Glycyrrhizin alleviates experimental allergic asthma in mice. Int Immunopharmacol. 2006;6(9):1468-77.
9. Fouladi S, Masjedi M, GanjalikhaniHakemi M, Eskandari N. The Review of in Vitro and in Vivo Studies over the Glycyrrhizic Acid as Natural Remedy Option for Treatment of Allergic Asthma. Iran J Allergy Asthma Immunol. 2019;18(1):1-11.
10. Kakegawa H, Matsumoto H, Satoh T. Inhibitory effects of some natural products on the activation of hyaluronidase and their anti-allergic actions. Chem Pharm Bull. 1992;40(6):1439-42.
11. Alshetaili A. Gefitinib loaded PLGA and chitosan coated PLGA nanoparticles with magnified cytotoxicity against A549 lung cancer cell lines. Saudi J Biol Sci. 2021; In Press.
12. Mir M, Ahmed N, Rehman AU. Recent applications of PLGA based nanostructures in drug delivery. Colloids Surf B Biointerfaces. 2017;159(2):217-31.
13. Ding D, Zhu Q. Recent advances of PLGA micro/nanoparticles for the delivery of biomacromolecular therapeutics. Mater Sci Eng. 2018;92(4):1041-60.
14. Athari SS, Mortaz E, Pourpak Z, Moin M, Moazzeni SM. VIP-loaded PLGA as an anti-asthma nanodrug candidate. Comp Clin Pathol. 2016;25(8):791–6.
15. Athari SS, Pourpak Z, Folkerts G, Garssen JO, Moin M, Adcock IM, et al. Conjugated Alpha-Alumina nanoparticle with vasoactive intestinal peptide as a Nano-drug in treatment of allergic asthma in mice. Eur J Pharmacol. 2016;15(791):811-20.
16. Kim SH, Hong JH, Lee JE, Lee YC. 18β-Glycyrrhetinic acid, the major bioactive component of Glycyrrhizae Radix, attenuates airway inflammation by modulating Th2 cytokines, GATA-3, STAT6, and Foxp3 transcription factors in an asthmatic mouse model. Environ ToxicolPharmacol. 2017;52(11):99-113.
17. Ploeger B, Mensinga T, Sips A, Seinen W, Meulenbelt J, DeJongh J. The pharmacokinetics of glycyrrhizic acid evaluated by physiologically based pharmacokinetic modeling. Drug Metab Rev. 2001;33(2):125–47.
18. Zhang HP, Wang L, Wang Z, Xu XR, Zhou XM, GLiu, et al. Chinese herbal medicine formula for acute asthma: A multi-center, randomized, double-blind, proof-of-concept trial. Respir Med. 2018;140(12):42–9.
19. N Yang N, S Patil S, J Zhuge J, MC Wen, Bolleddula J, Doddaga S, et al. Glycyrrhizauralensis flavonoids present in antiasthma formula, ASHMI™️, inhibit memory Th2 responses in vitro and in vivo.Phytother Res. 2013;27(9):1381–91.
20. Liu Z, Wang P, Lu S, Guo R, Gao W, Tong H, et al. Liquiritin, a novel inhibitor of TRPV1 and TRPA1, protects against LPS-induced acute lung injury. Cell Calcium. 2020;88(12):102198.
21. Isbrucker RA, Burdock GA. Risk and safety assessment on the consumption of Licorice root (Glycyrrhiza sp.), its extract and powder as a food ingredient, with emphasis on the pharmacology and toxicology of glycyrrhizin. RegulToxicolPharmacol. 2006;46(3):167–92.
22. Fu Y, Chen J, Li YJ, Zheng YF, Li P. Antioxidant and anti-inflammatory activities of six flavonoids separated from licorice. Food Chem. 2013;141(2):1063–71.
23. Haraguchi H, Ishikawa H, Mizutani K, Tamura Y, Kinoshita T. Antioxidative and superoxide scavenging activities of retrochalcones in Glycyrrhiza inflata. Bioorg Med Chem. 1998;6(3):339–47.
24. Zhang Y, Luo H, Liu K, Jia H, Chen Y, Wang Z. Antioxidant effects of liquorice (Glycyrrhiza uralensis) extract during aging of longissimus thoracis muscle in Tan sheep. Meat Sci. 2015;105(7):38-45.
25. Zhang YW, Luo HL, Chang YF, Jiao LJ, Liu K. Effects of liquorice extract on the activity and gene expression level of antioxidant enzymes in longissimus dorsi muscle of Tan lamb. Small Rumin Res. 2017;154(5):23-8.
| Files | ||
| Issue | Vol 21 No 1 (2022) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijaai.v21i1.8617 | |
| Keywords | ||
| Allergy and immunology Asthma Medicinal plants Nanoparticles Traditional medicine | ||
| Rights and permissions | |
|
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
