Normal Range Determination of Lymphocytes Subsets in Normal Adults in Iran


Immunophenotyping of lymphocytes is very essential for evaluation of immune system. Due  to  the  effect  of  environmental  factors  and  ethnical diversity on  immune  system, establishment of an internal normal range of lymphocyte subsets is a necessity for each population.  The  aim  of  this  study  was  to  determine  the  normal  range  of  T  and  B lymphocytes, and NK cells in normal Iranian adults.
Two hundred and thirty three Iranian normal adult volunteers took part in this study. Complete Blood Count (CBC) was performed for them with Sysmex (KX21) and cells with CD3, CD4, CD8, CD19 and CD16/56  surface markers were simultaneously detected by flow cytometry method  with FACstar system. Their percentile and absolute count  were determined.
The  volunteers  were  150  male  and  83  female.  Mean  percentages  of  lymphocyte subpopulation were: CD3 (67.66 ±7.76), CD19 (14.41±5.09), CD4 (39.22±6.7), CD8 (25.42±5.4) and CD16/56 (10.14±6.42). Also, their mean absolute count of lymphocyte bearing CD3, CD19, CD4 and CD8 were 1,504±505/µl, 332±186/µl, 827±313/µl and 522±185/µl, respectively.
Our results are comparable with similar Asian results from other Asian population, but are different from European population, we therefore conclude that it is necessary for each laboratory to establish an internal normal range for the lymphocytes bearing above- mentioned markers.

Cram LS. Flow cytometry, an overview. Methods Cell Sci 2002; 24(1-3):1-9.
2. Al Qouzi A, Al Salamah A, Al Rasheed R, Al Musalam A, Al Khairy K, Kheir O, et al. Immunophenotyping of peripheral blood lymphocytes in Saudi men. Clin Diagn Lab Immunol 2002; 9(2):279-81.
3. Ekong T, Gompels M, Clark C, Parkin J, Pinching A. Double-staining artefact observed in certain individuals during dual-colour immunophenotyping of lymphocytes by flow cytometry. Cytometry 1993; 14(6):679-84.
4. Jentsch-Ullrich K, Koenigsmann M, Mohren M, Franke A. Lymphocyte subsets' reference ranges in an age- and gender-balanced population of 100 healthy adults--a monocentric German study. Clin Immunol 2005;116(2):192-7.
5. Brown M, Wittwer C. Flow cytometry: principles and clinical applications in hematology. Clin Chem 2000;46(8 Pt 2):1221-9.
6. Katevas P, Lembesopoulios C, Kamitaki C, Sakellarious D, Douna V. Establishment of normal ranges of lymphocyte subpopulation in cord blood and in peripheral blood in infants and in children: Haema 1999;2(3):145-151.
7. Webster HK, Kawai Y, Haney D, Nakahara K, Watanabe K, Ogawa E, et al. Determination of laboratory reference ranges for lymphocyte immunophenotype subsets in healthy adult Japanese using flow cytometry. Asian Pac J Allergy Immunol 2003; 21(3):161-9.
8. Tsegaye A, Messele T, Tilahun T, Hailu E, Sahlu T,Doorly R, et al. Immunohematological reference ranges for adult Ethiopians. Clin Diagn Lab Immunol 1999;6(3):410-4.
9. Amatya R, Vajpayee M, Kaushik S, Kanswal S, Pandey RM, Seth P. Lymphocyte immunophenotype reference ranges in healthy Indian adults: implications for management of HIV/AIDS in India. Clin Immunol 2004;112(3):290-5.
10. Kam KM, Leung WL, Kwok MY, Hung MY, Lee SS, Mak WP. Lymphocyte subpopulation reference ranges for monitoring human immunodeficiency virus-infected Chinese adults. Clin Diagn Lab Immunol 1996; 3(3):326-30.
11. Bisset LR, Lung TL, Kaelin M, Ludwig E, Dubs RW.Reference values for peripheral blood lymphocyte phenotypes applicable to the healthy adult population in Switzerland. Eur J Haematol 2004; 72(3):203-12.
12. McCoy JP Jr, Overton WR. Quality control in flow cytometry for diagnostic pathology: II. A conspectus of reference ranges for lymphocyte immunophenotyping. Cytometry 1994; 18(3):129-39.
13. Howard RR, Fasano CS, Frey L, Miller CH. Reference Intervals of CD3, CD4, CD8, CD4/CD8, and Absolute CD4 Values in Asian and Non-Asian Populations. Cytometry (Communications in Clinical Cytometry)1996; 26: 231-232.
14. Yaman A, Cetiner S, Kibar F, Tasova Y, Seydaoglu G, Dundar IH. Reference ranges of lymphocyte subsets of healthy adults in Turkey. Med Princ Pract 2005;14(3):189-93.
15. Chng WJ, Tan GB, Kuperan P. Establishment of adult peripheral blood lymphocyte subset reference range for an Asian population by single-platform flow cytometry: influence of age, sex, and race and comparison with other published studies. Clin Diagn Lab Immunol 2004; 11(1):168-73.
16. Rudy BJ, Wilson CM, Durako S, Moscicki AB, Muenz L, Douglas SD. Peripheral blood lymphocyte subsets in adolescents: a longitudinal analysis from the REACH project. Clin Diagn Lab Immunol 2002; 9(5):959-65.

IssueVol 10, No 4 (2011) QRcode
Immunophenotyping CD Marker Flow Cytometry Lymphocyte Normal Range NK cell

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
Shokouhi Shoormasti R, Azimdoost A, Saghafi S, Movahhedi M, Haghi Ashtiani MT, Pourpak Z, Eslami MB. Normal Range Determination of Lymphocytes Subsets in Normal Adults in Iran. Iran J Allergy Asthma Immunol. 1;10(4):295-298.