Immunochemical Characterization of Ligustrum Vulgare (Privet) Pollen Allergens: Study of Common Allergenic Plant in Iran
-
Haleh Abdoli Sereshki
,
Reza Falak
,
Mohammad-Ali Assarehzadegan
,
Mohammad Nabavi
,
Mohammad-Hossein Shams
,
Maral Ranjbar
Abstract
Ligustrum vulgare (Privet) pollen proteins are responsible for allergies in susceptible individuals in many regions of the world. This study investigated the immunochemical characterization of Privet pollen extract and the occurrence of skin prick test reactivity to Privet and other allergenic pollen grains in allergic rhinitis patients.
All subjects experienced a skin prick test with twenty-two allergen extracts. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) separated Privet pollen extract, IgE-immunoblotting, and specific ELISA procedures determined the allergenic profile on forty-five Privet allergic patients.
A positive allergic reaction to L. vulgare pollen extract was observed in forty-five (31.4%) out of 145 patients. Ten resolved protein fractions were found on SDS-PAGE, ranging from 10 to 80 kDa. IgE-specific antibodies interacted with several allergenic protein bands from Privet-allergic patients in the immunoblotting assay. The most significant interaction was observed in proteins with molecular weights of approximately 15, 18, 43, and 66 kDa.
Privet pollen is regarded as a potent allergen composed of IgE-binding constituents. Considering the high allergenicity of Privet pollen grains and since many countries are rich in this plant, identification and production of recombinant forms of common allergens in this species can be used for developing more efficient diagnostic, therapeutic, and preventive approaches.
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2. Asam C, Hofer H, Wolf M, Aglas L, Wallner M. Tree pollen allergens—an update from a molecular perspective. Allergy. 2015;70(10):1201-11.
3. Shoushtari MS, Majd A, Assarehzadegan MA, Fanuel S, Moin M, Nejadsattari T, et al. Identification of a 53 kDa protein, as a new high molecular weight allergen from Fraxinus excelsior (Ash) pollen. Allergo J Int. 2020;29(7):233-9.
4. Villalba M, Rodríguez R,Batanero E. The spectrum of olive pollen allergens. From structures to diagnosis and treatment. Methods. 2014;66(1):44-54.
5. Cariñanos P, Alcázar P, Galán C, Domínguez E. Privet pollen (Ligustrum sp.) as potential cause of pollinosis in the city of Cordoba, south‐west Spain. Allergy. 2002;57(2):92-7.
6. Robledo-Retana T, Mani BM, Teran LM. Ligustrum pollen: New insights into allergic disease. World Allergy Organ J. 2020;13(2):100104.
7. González MF,Candau Fernández-Mensaque M. Variations of airborne summer pollen in southwestern Spain. J InvestigAllergol Clin Immunol. 1994;4(6):277-82.
8. Ahvazi M, Khalighi-Sigaroodi F, Charkhchiyan MM, Mojab F, Mozaffarian V-A, Zakeri H. Introduction of medicinal plants species with the most traditional usage in Alamut region.IJPR. 2012;11(1):185
9. Mandal J, Roy I, Chatterjee S, Gupta-Bhattacharya S. Aerobiological investigation and in vitro studies of pollen grains from 2 dominant avenue trees in Kolkata, India.J InvestigAllergol Clin Immunol. 2008;18(1):22-30.
10. Valenta R, Kraft D. From allergen structure to new forms of allergen-specific immunotherapy. CurrOpinImmunol. 2002;14(6):718-27.
11. Vrinceanu D, Berghi ON, Cergan R, Dumitru M, Ciuluvica RC, Giurcaneanu C, et al. Urban allergy review: Allergic rhinitis and asthma with plane tree sensitization. ExpTher Med. 2021;21(3):1-.
12. Amini A, Sankian M, Assarehzadegan MA, Vahedi F, Varasteh A. Chenopodium album pollen profilin (Che a 2): homology modeling and evaluation of cross-reactivity with allergenic profilins based on predicted potential IgE epitopes and IgE reactivity analysis. Mol Biol Rep. 2011;38(4):2579-87.
13. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976;72(5):248-54.
14. Assarehzadegan MA, Shakurnia A,Amini A. The most common aeroallergens in a tropical region in Southwestern Iran. World Allergy Organ J. 2013;6(1):1-7.
15. Khosravi GR, Assarehzadegan MA, Morakabati P, Akbari B, Dusti F. Cloning and expression of Aca f 1: a new allergen of Acacia farnesiana pollen. Cent Eur J Immunol. 2016;41(3):273-9.
16. Assarehzadegan MA, Sankian M, Jabbari F, Tehrani M, Varasteh A. Expression of the recombinant major allergen of Salsola kali pollen (Sal k 1) and comparison with its low-immunoglobulin E-binding mutant. Allergol Int. 2010;59(2):213-22.
17. Shams MH, Assarehzadegan MA, Eskandari N, Masjedi M, Kheirandish F, Ghasemi R, et al. Molecular and Immunochemical Characterization of Pop n 2: A New Allergen of Populus nigra Pollen. Clin Exp Allergy. 2020.
18. Assarehzadegan, MA, Shakurnia A,Amini A. The most common aeroallergens in a tropical region in Southwestern Iran. Cent Eur J Immunol. 2013;6(1):7-11.
19. Fereidouni M, Hossini RF, Azad FJ, Assarehzadegan MA, Varasteh A. Skin prick test reactivity to common aeroallergens among allergic rhinitis patients in Iran. Allergolimmunopathol. 2009;37(2):73-9.
20. Payandeh P, Fadaee J, Azad FJ, Bakhshaii M, Sistani S. Allergens prevalence among patients with respiratory allergies in Mashhad, Iran. Tanaffos. 2019;18(2):133-9.
21. Tehrani M, Sankian M, Assarehzadegan MA, Falak R, JabbariF, Varasteh A. Immunochemical Characterization of Amaranthus retroflexus Pollen Extract: Extensive Cross-reactive Allergenic Components among the Four Species of Amaranthaceae/Chenopodiaceae. Iranian JAllergy Asthma Immunol.2010;9(2):87-95.
22. Pendino P, Aguero C, Cavagnero P, Lopez K, Kriunis I, Molinas J. Aeroallergen sensitization in wheezing children from Rosario, Argentina. World Allergy Organ J. 2011;4(10):159-63.
23. Morfin-Maciel BM, Flores I, Rosas-Alvarado A, Bautista M, Lopez-Lopez JR. Sensitization to pollens of oleaceae family in a group of patients from Mexico CityRevAlergMex. 2009;56(6):194-9.
24. Monroy-Colín A, Silva-Palacios I, Tormo-Molina R, Maya-Manzano JM, Rodríguez SF, Gonzalo-Garijo Á. Environmental analysis of airborne pollen occurrence, pollen source distribution and phenology of Fraxinus angustifolia. Aerobiologia. 2018;34(3):269-83.
25. Bicakci A,Tosunoglu A. Allergenic Pollens in Turkey. Asthma Allergy Immunol. 2019;17(1):7-24.
26. Mansouritorghabeh H, Jabbari-Azad F, Sankian M, Varasteh A, Farid-Hosseini R. The most common allergenic tree pollen grains in the middle east: a narrative review.Iran J Med Sci. 2019;44(2):87-91.
27. Robledo-Retana T, Zenteno E, Agundis-Mata M, Pereyra-Morales M, Calderón-Segura M, Calderón-Ezquerro M. Detection of immunogens from Fraxinus spp. pollen grains. Aerobiologia. 2015;31(3):403-10.
28. Bedolla-Barajas M, Bedolla-Pulido T, Flores-Merino M, Jiménez-Rosales A, Domínguez-García M. Oral allergy syndrome amongst young Mexicans: prevalence and associated factors. Eur Ann Allergy Clin Immunol. 2018;51(1):15-20.
29. Huby RD, Dearman RJ, Kimber I. Why are some proteins allergens? ToxicolSci. 2000;55(2):235-46.
30. Sen M, Kopper R, Pons L, Abraham EC, Burks AW, Bannon GA. Protein Structure Plays a Critical Role in Peanut Allergen Stability and May Determine Immunodominant IgE-Binding Epitopes. J Immunol. 2002;169(2):882-7.
31. Assarehzadegan MA, Sankian M, Jabbari F, Tehrani M, Falak R, Varasteh A. Identification of methionine synthase (Sal k 3), as a novel allergen of Salsola kali pollen. MolBiolRep. 2011;38(1):65-73.
32. Assarehzadegan MA, Khodadadi A, Amini A, Shakurnia AH, Marashi SS, Ali-Sadeghi H, et al. Immunochemical characterization of Prosopis juliflora pollen allergens and evaluation of cross-reactivity pattern with the most allergenic pollens in tropical areas.Iranian J Allergy Asthma Immunol. 2014;14(1):74-82.
33. Assarehzadegan MA, Amini A, Sankian M, Tehrani M, Jabbari F, Varasteh A. Sal k 4, a new allergen of Salsola kali, is profilin: a predictive value of conserved conformational regions in cross-reactivity with other plant-derived profilins.Biosci.BiotechnolBiochem. 2010;74(7):1441-6.
34. Mas García S, Garrido Arandia M, Batanero Cremades E, Purohit A, Pauli G, Rodríguez García R, et al. Characterization of profilin and polcalcin panallergens from ash pollen. J InvestigAllergol Clin Immunol. 2014;24(4):257-66.
35. Ali-Sadeghi H, Khodadadi A, Amini A, Assarehzadegan MA, Sepahi N, Zarinhadideh F. Pro j 2 is mesquite profilin: molecular characteristics and specific IgE binding activity. Asian Pac J Allergy Immunol. 2015;33(2):90-8.
36. Assarehzadegan MA, Khodadadi A, Amini A, Shakurnia AH, Marashi SS, Ali-Sadeghi H, et al. Immunochemical characterization of prosopis juliflora pollen allergens and evaluation of cross-reactivity pattern with the most allergenic pollens in tropical areas. Iranian J Allergy Asthma Immunol. 2015;14(1):74-82.
37. Barderas R, Purohit A, Papanikolaou I, Rodríguez R, Pauli G,Villalba M. Cloning, expression, and clinical significance of the major allergen from ash pollen, Fra e1.J Allergy Clin Immunol. 2005;115(2):351-7.
38. Asturias JA, Arilla MC, Gomez-Bayon N, Martinez J, Martinez A, Palacios R. Cloning and expression of the panallergen profilin and the major allergen (Ole e 1) from olive tree pollen. J Allergy Clin Immunol. 1997;100(3):365-72.
39. Gonzalez E, Villalba M, Rodriguez R. Immunological and molecular characterization of the major allergens from lilac and privet pollens overproduced in Pichia pastoris.Clin Exp Allergy. 2001;31(2):313-21.
40. Batanero E, De La Pena MAG, Villalba M, Monsalve RI, Martinâ€Esteban M,RodrÃguez R. Isolation, cDNA cloning and expression of Lig v 1, the major allergen from privet pollen. Clin Exp Allergy. 1996;26(12):1401-10.
41. DÃaz FnJn. Allergen Profile of London Plane Tree Pollen: Clinical and Molecular Pattern in Central Spain. J InvestigAllergol Clin Immunol. 2022;32(5).
42. Osada T, Okano M. Japanese cedar and cypress pollinosis updated: New allergens, cross-reactivity, and treatment. Allergol Int. 2021.
43. Assarehzadegan MA, Sankian M, Jabbari F, Noorbakhsh R, Varasteh A. Allergy to Salsola Kali in a Salsola incanescens-rich area: role of extensive cross allergenicity. Allergol Int. 2009;58(2):261-6.
44. Shamsbiranvand MH, Khodadadi A, Assarehzadegan MA, Borci SH, Amini A. Immunochemical characterization of acacia pollen allergens and evaluation of cross-reactivity pattern with the common allergenic pollens. J Allergy (Cairo). 2014;2014:409056.
45. Sharif Shoushtari M, Majd A, Pourpak Z, Shahali Y, Moin M,Eslami MB. Differential allergenicity of mature and immature pollen grains in Shasta daisy (Chrysanthemum maximum Ramond). Iranian J Allergy Asthma Immunol. 2013;12(2):99-106.
46. Zarinhadideh F, Amini A, Assarehzadegan MA, Borsi SH, Sepahi N, Ali-Sadeghi H. Immunochemical and molecular characterization of allergenic profilin (Koc s 2) from Kochia scoparia pollen. J Appl Biol Chem. 2015;58(3):443-51.
2. Asam C, Hofer H, Wolf M, Aglas L, Wallner M. Tree pollen allergens—an update from a molecular perspective. Allergy. 2015;70(10):1201-11.
3. Shoushtari MS, Majd A, Assarehzadegan MA, Fanuel S, Moin M, Nejadsattari T, et al. Identification of a 53 kDa protein, as a new high molecular weight allergen from Fraxinus excelsior (Ash) pollen. Allergo J Int. 2020;29(7):233-9.
4. Villalba M, Rodríguez R,Batanero E. The spectrum of olive pollen allergens. From structures to diagnosis and treatment. Methods. 2014;66(1):44-54.
5. Cariñanos P, Alcázar P, Galán C, Domínguez E. Privet pollen (Ligustrum sp.) as potential cause of pollinosis in the city of Cordoba, south‐west Spain. Allergy. 2002;57(2):92-7.
6. Robledo-Retana T, Mani BM, Teran LM. Ligustrum pollen: New insights into allergic disease. World Allergy Organ J. 2020;13(2):100104.
7. González MF,Candau Fernández-Mensaque M. Variations of airborne summer pollen in southwestern Spain. J InvestigAllergol Clin Immunol. 1994;4(6):277-82.
8. Ahvazi M, Khalighi-Sigaroodi F, Charkhchiyan MM, Mojab F, Mozaffarian V-A, Zakeri H. Introduction of medicinal plants species with the most traditional usage in Alamut region.IJPR. 2012;11(1):185
9. Mandal J, Roy I, Chatterjee S, Gupta-Bhattacharya S. Aerobiological investigation and in vitro studies of pollen grains from 2 dominant avenue trees in Kolkata, India.J InvestigAllergol Clin Immunol. 2008;18(1):22-30.
10. Valenta R, Kraft D. From allergen structure to new forms of allergen-specific immunotherapy. CurrOpinImmunol. 2002;14(6):718-27.
11. Vrinceanu D, Berghi ON, Cergan R, Dumitru M, Ciuluvica RC, Giurcaneanu C, et al. Urban allergy review: Allergic rhinitis and asthma with plane tree sensitization. ExpTher Med. 2021;21(3):1-.
12. Amini A, Sankian M, Assarehzadegan MA, Vahedi F, Varasteh A. Chenopodium album pollen profilin (Che a 2): homology modeling and evaluation of cross-reactivity with allergenic profilins based on predicted potential IgE epitopes and IgE reactivity analysis. Mol Biol Rep. 2011;38(4):2579-87.
13. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976;72(5):248-54.
14. Assarehzadegan MA, Shakurnia A,Amini A. The most common aeroallergens in a tropical region in Southwestern Iran. World Allergy Organ J. 2013;6(1):1-7.
15. Khosravi GR, Assarehzadegan MA, Morakabati P, Akbari B, Dusti F. Cloning and expression of Aca f 1: a new allergen of Acacia farnesiana pollen. Cent Eur J Immunol. 2016;41(3):273-9.
16. Assarehzadegan MA, Sankian M, Jabbari F, Tehrani M, Varasteh A. Expression of the recombinant major allergen of Salsola kali pollen (Sal k 1) and comparison with its low-immunoglobulin E-binding mutant. Allergol Int. 2010;59(2):213-22.
17. Shams MH, Assarehzadegan MA, Eskandari N, Masjedi M, Kheirandish F, Ghasemi R, et al. Molecular and Immunochemical Characterization of Pop n 2: A New Allergen of Populus nigra Pollen. Clin Exp Allergy. 2020.
18. Assarehzadegan, MA, Shakurnia A,Amini A. The most common aeroallergens in a tropical region in Southwestern Iran. Cent Eur J Immunol. 2013;6(1):7-11.
19. Fereidouni M, Hossini RF, Azad FJ, Assarehzadegan MA, Varasteh A. Skin prick test reactivity to common aeroallergens among allergic rhinitis patients in Iran. Allergolimmunopathol. 2009;37(2):73-9.
20. Payandeh P, Fadaee J, Azad FJ, Bakhshaii M, Sistani S. Allergens prevalence among patients with respiratory allergies in Mashhad, Iran. Tanaffos. 2019;18(2):133-9.
21. Tehrani M, Sankian M, Assarehzadegan MA, Falak R, JabbariF, Varasteh A. Immunochemical Characterization of Amaranthus retroflexus Pollen Extract: Extensive Cross-reactive Allergenic Components among the Four Species of Amaranthaceae/Chenopodiaceae. Iranian JAllergy Asthma Immunol.2010;9(2):87-95.
22. Pendino P, Aguero C, Cavagnero P, Lopez K, Kriunis I, Molinas J. Aeroallergen sensitization in wheezing children from Rosario, Argentina. World Allergy Organ J. 2011;4(10):159-63.
23. Morfin-Maciel BM, Flores I, Rosas-Alvarado A, Bautista M, Lopez-Lopez JR. Sensitization to pollens of oleaceae family in a group of patients from Mexico CityRevAlergMex. 2009;56(6):194-9.
24. Monroy-Colín A, Silva-Palacios I, Tormo-Molina R, Maya-Manzano JM, Rodríguez SF, Gonzalo-Garijo Á. Environmental analysis of airborne pollen occurrence, pollen source distribution and phenology of Fraxinus angustifolia. Aerobiologia. 2018;34(3):269-83.
25. Bicakci A,Tosunoglu A. Allergenic Pollens in Turkey. Asthma Allergy Immunol. 2019;17(1):7-24.
26. Mansouritorghabeh H, Jabbari-Azad F, Sankian M, Varasteh A, Farid-Hosseini R. The most common allergenic tree pollen grains in the middle east: a narrative review.Iran J Med Sci. 2019;44(2):87-91.
27. Robledo-Retana T, Zenteno E, Agundis-Mata M, Pereyra-Morales M, Calderón-Segura M, Calderón-Ezquerro M. Detection of immunogens from Fraxinus spp. pollen grains. Aerobiologia. 2015;31(3):403-10.
28. Bedolla-Barajas M, Bedolla-Pulido T, Flores-Merino M, Jiménez-Rosales A, Domínguez-García M. Oral allergy syndrome amongst young Mexicans: prevalence and associated factors. Eur Ann Allergy Clin Immunol. 2018;51(1):15-20.
29. Huby RD, Dearman RJ, Kimber I. Why are some proteins allergens? ToxicolSci. 2000;55(2):235-46.
30. Sen M, Kopper R, Pons L, Abraham EC, Burks AW, Bannon GA. Protein Structure Plays a Critical Role in Peanut Allergen Stability and May Determine Immunodominant IgE-Binding Epitopes. J Immunol. 2002;169(2):882-7.
31. Assarehzadegan MA, Sankian M, Jabbari F, Tehrani M, Falak R, Varasteh A. Identification of methionine synthase (Sal k 3), as a novel allergen of Salsola kali pollen. MolBiolRep. 2011;38(1):65-73.
32. Assarehzadegan MA, Khodadadi A, Amini A, Shakurnia AH, Marashi SS, Ali-Sadeghi H, et al. Immunochemical characterization of Prosopis juliflora pollen allergens and evaluation of cross-reactivity pattern with the most allergenic pollens in tropical areas.Iranian J Allergy Asthma Immunol. 2014;14(1):74-82.
33. Assarehzadegan MA, Amini A, Sankian M, Tehrani M, Jabbari F, Varasteh A. Sal k 4, a new allergen of Salsola kali, is profilin: a predictive value of conserved conformational regions in cross-reactivity with other plant-derived profilins.Biosci.BiotechnolBiochem. 2010;74(7):1441-6.
34. Mas García S, Garrido Arandia M, Batanero Cremades E, Purohit A, Pauli G, Rodríguez García R, et al. Characterization of profilin and polcalcin panallergens from ash pollen. J InvestigAllergol Clin Immunol. 2014;24(4):257-66.
35. Ali-Sadeghi H, Khodadadi A, Amini A, Assarehzadegan MA, Sepahi N, Zarinhadideh F. Pro j 2 is mesquite profilin: molecular characteristics and specific IgE binding activity. Asian Pac J Allergy Immunol. 2015;33(2):90-8.
36. Assarehzadegan MA, Khodadadi A, Amini A, Shakurnia AH, Marashi SS, Ali-Sadeghi H, et al. Immunochemical characterization of prosopis juliflora pollen allergens and evaluation of cross-reactivity pattern with the most allergenic pollens in tropical areas. Iranian J Allergy Asthma Immunol. 2015;14(1):74-82.
37. Barderas R, Purohit A, Papanikolaou I, Rodríguez R, Pauli G,Villalba M. Cloning, expression, and clinical significance of the major allergen from ash pollen, Fra e1.J Allergy Clin Immunol. 2005;115(2):351-7.
38. Asturias JA, Arilla MC, Gomez-Bayon N, Martinez J, Martinez A, Palacios R. Cloning and expression of the panallergen profilin and the major allergen (Ole e 1) from olive tree pollen. J Allergy Clin Immunol. 1997;100(3):365-72.
39. Gonzalez E, Villalba M, Rodriguez R. Immunological and molecular characterization of the major allergens from lilac and privet pollens overproduced in Pichia pastoris.Clin Exp Allergy. 2001;31(2):313-21.
40. Batanero E, De La Pena MAG, Villalba M, Monsalve RI, Martinâ€Esteban M,RodrÃguez R. Isolation, cDNA cloning and expression of Lig v 1, the major allergen from privet pollen. Clin Exp Allergy. 1996;26(12):1401-10.
41. DÃaz FnJn. Allergen Profile of London Plane Tree Pollen: Clinical and Molecular Pattern in Central Spain. J InvestigAllergol Clin Immunol. 2022;32(5).
42. Osada T, Okano M. Japanese cedar and cypress pollinosis updated: New allergens, cross-reactivity, and treatment. Allergol Int. 2021.
43. Assarehzadegan MA, Sankian M, Jabbari F, Noorbakhsh R, Varasteh A. Allergy to Salsola Kali in a Salsola incanescens-rich area: role of extensive cross allergenicity. Allergol Int. 2009;58(2):261-6.
44. Shamsbiranvand MH, Khodadadi A, Assarehzadegan MA, Borci SH, Amini A. Immunochemical characterization of acacia pollen allergens and evaluation of cross-reactivity pattern with the common allergenic pollens. J Allergy (Cairo). 2014;2014:409056.
45. Sharif Shoushtari M, Majd A, Pourpak Z, Shahali Y, Moin M,Eslami MB. Differential allergenicity of mature and immature pollen grains in Shasta daisy (Chrysanthemum maximum Ramond). Iranian J Allergy Asthma Immunol. 2013;12(2):99-106.
46. Zarinhadideh F, Amini A, Assarehzadegan MA, Borsi SH, Sepahi N, Ali-Sadeghi H. Immunochemical and molecular characterization of allergenic profilin (Koc s 2) from Kochia scoparia pollen. J Appl Biol Chem. 2015;58(3):443-51.
| Files | ||
| Issue | Vol 21 No 1 (2022) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijaai.v21i1.8615 | |
| Keywords | ||
| Allergens Iran Ligustrum Prevalence Pollen | ||
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How to Cite
1.
Abdoli Sereshki H, Falak R, Assarehzadegan M-A, Nabavi M, Shams M-H, Ranjbar M. Immunochemical Characterization of Ligustrum Vulgare (Privet) Pollen Allergens: Study of Common Allergenic Plant in Iran. Iran J Allergy Asthma Immunol. 2022;21(1):55-64.
