Expression of IL-17 and COX2 Gene in Peripheral Blood Leukocytes of Vitiligo Patients
-
Behnaz Esmaeili
,
Seyed Abdol Rahim Rezaee
,
Pouran Layegh
,
Jalil Tavakkol Afshari
,
Phil Dye
,
Ehsan Ghayoor Karimiani
,
Fatemeh Kalalinia
,
Houshang Rafatpanah
Abstract
Vitiligo is a pigmentation disorder in which inflammatory mediators such as cytokines have a pivotal role in disease's pathogenesis. Interleukin 17 (IL-17A) is a proinflammatory cytokine which is involved in the induction of several proinflamatory mediators such as cyclooxygenase 2 (COX2). The aim of this study was to investigate the gene expression of IL-17 and COX2 in peripheral blood leukocytes of vitiligo's patients.
Peripheral blood leukocytes from 15 patients with vitiligo and 15 healthy controls were separated using a gradient density centrifugation method. After total RNA isolation and cDNA synthesis, IL-17 and COX2 gene expression were quantified by real-time polymerase chain reaction (PCR).
There were no significant differences in IL-17 and COX2 gene expression in lymphocytes of patients with vitiligo compared with control group (p<0.05). However there was an increased IL-17 and COX2 gene expression in neutrophils of patients compared to controls, but it did not reach statistical significance (p=0.05). We could not find any differences in IL-17 and Cox2 gene expression between clinical diseases subtypes, sex and age. There was a significant correlation between IL-17 and COX2 genes expression in the neutrophils of patients (p=0.00, r=0.80).
Our results showed an increased expression in IL-17 and Cox-2 genes in neurophils of patients with vitiligo. This suggested that these two factors are involved in the inflammatory process. Further studies with a larger sample size might help to establish the role of these factors in the pathogenesis of diseases.
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| Issue | Vol 10, No 2 (2011) | |
| Section | Articles | |
| Keywords | ||
| COX2 IL-17 Neutrophils Real-time PCR Vitiligo | ||
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