The Effects of Particulate Matter on C57BL/6 Peritoneal and Alveolar Macrophages

  • Hoda Rahmani ORCID GS PM Department of Immunology, Medicine Faculty, Shahid Beheshti University of Medical Science, Tehran, Iran
  • Somaye Sadeghi ORCID GS PM Department of Immunology, Medicine Faculty, Shahid Beheshti University of Medical Science, Tehran, Iran
  • Niloofar Taghipour ORCID GS PM Department of Tissue Engineering and Applied Cell Science, School of Advanced Technologies in Medicine, Shahid Beheshti University of medical science, Tehran. Iran
  • Mohsen Roshani ORCID GS PM Tehran Air Quality Control Company (AQCC), Tehran, Iran
  • Davar Amani ORCID GS PM Department of Immunology, Medicine Faculty, Shahid Beheshti University of Medical Science, Tehran, Iran
  • Tooba Ghazanfari ORCID GS PM Department of Immunology, School of Medicine, Shahed University of Medical Science, Tehran, Iran
  • Nariman Mosaffa ORCID GS PM Mail Department of Immunology, Medicine Faculty, Shahid Beheshti University of Medical Science, Tehran, Iran
Keywords:
Inflammation, Macrophages, Particulate matter

Abstract

The presence of ambient particulate matter (PM) poses more dangers to human health than that of other common air pollutants such as Carbon dioxide (Co2) and ozone.  Epidemiologic studies show a direct correlation between PM and the risk of respiratory and cardiovascular diseases. The immune system seems to play a critical role in the process of these diseases. The main goal of this study was to investigate the effect of Tehran particulate matter in two aerodynamic diameters (PM2.5 and PM10) on alveolar macrophages (AM) from C57/BL6 mice. To evaluate the inflammatory effects of PMs, cultured alveolar, and peritoneal macrophages were treated with PM2.5 and PM10 (concentrations of 5 µg/mL and 10 µg/mL). Tumor necrosis factor-alpha (TNF-α) and IL-10 (representatives of inflammatory and anti-inflammatory cytokines, respectively) were assessed in the culture supernatant by ELISA. Expression of arginase and inducible nitric oxide synthase (iNOS) genes was carried out by quantitative real-time PCR. Different functional types of cultured alveolar macrophages (M1, M2) were also determined in this study. Our results suggest that PM2.5 induces M1 inflammatory phenotype in comparison with PM10. We found Also, an increase in TNF-α and M1-related gene expression (iNOS), as well as a decrease in both IL-10 and M2 phenotype genes (Arginase). Moreover, a reduction in phagocytic capacity and increased apoptosis function of macrophage cells were detected. PM2.5 as a major component in hydrocarbons has a considerable effect on polarizing the alveolar macrophages to an inflammatory phenotype and eliciting lung inflammation in mice.

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Published
2020-12-19
How to Cite
1.
Rahmani H, Sadeghi S, Taghipour N, Roshani M, Amani D, Ghazanfari T, Mosaffa N. The Effects of Particulate Matter on C57BL/6 Peritoneal and Alveolar Macrophages. Iran J Allergy Asthma Immunol. 19(6):647-659.
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