Enhancement of Anti-allergic Effect of Diethylcarbamazine Citrate in Asthmatic Mouse Model: Testing of Anti-drug Antibodies and Quercetin
,
Abstract
Diethylcarbamazine citrate (DEC) is known as an effective treatment for bronchial asthma because of its ability to reduce eosinophil trafficking to the lung tissue. The current study aimed to potentiate the anti-allergic effect of the drug by passive immunization of the asthmatic model with anti-DEC antibody or prior treatment with quercetin (Qur).
Eight mice groups were categorized into control, the model of lung asthma, treated with DEC, passively immunized with anti(α)-bovine serum albumin Ab, anti-DEC Ab, prior exposure to 10, 20, or 40 mg Qur/Kg. b.wt. Both eosinophil peroxidase (EPO) and eotaxin2 in the lung tissues were performed. Serum levels of cytokines, bronchoalveolar lavage fluid (BALF) IgE, rabbit anti-bovine serum albumin (anti-BSA), and DEC IgG in lung tissue homogenates were assayed by ELISA.
Regarding the effect of anti-DEC Ab and Qur on DEC-induced recovery of histopathological alterations showed that the Ova group had peri-bronchial hyperplasia, mononuclear leukocyte infiltration, thickening in the wall of alveoli, and congested blood vessels. However, the reduction of inflammatory cells and thickened alveolar walls was dependent on the Qur dose. Qur40 enhanced the anti-allergic effect of DEC. Moreover, the present data revealed high levels of Th2 cytokines (IL-4 and IL-5) and IgE in the Ova group. An increased leukocyte infiltration/thickening of the alveolar wall and lung tissue EPO/eotaxin2 were also observed. Qur-40 could show an enhancement effect on DEC for the reduction of IL-4, IL-5, IgE, EPO, and eotaxin 2. Consequently, the IFN-γ/IL-4 ratio was increased.
Qur at 40 mg/Kg could be recommended to enhance the DEC effect suggesting a novel approach for treatment.
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3. Acharya KR, Ackerman SJ. Eosinophil granule proteins: form and function. J Biol Chem. 2014;289(25):17406-15.
4. Corry DB, Grünig G, Hadeiba H, Kurup VP, Warnock ML, Sheppard D, et al. Requirements for allergen-induced airway hyperreactivity in T and B cell-deficient mice. Mol Med.1998;4(5):344-55.
5. Barnes PJ. Glucocorticosteroids: current and future directions. Brit J Pharm.2011;163(1):29-43.
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7. Florêncio M, Saraiva K, Peixoto C. The effects of diethylcarbamazine on the ultrastructure of lung cells in vivo. Tiss Cell. 2005;37(3):241-6.
8. Tsanglao WR, Nandan D, Chandelia S, Arya NK, Sharma A. Filarial tropical pulmonary eosinophilia: a condition masquerading asthma, a series of 12 cases. J Asthma.2019;56(7):791-8.
9. Mukhopadhyay S, Ravindran B. Antibodies to diethylcarbamazine potentiate the antifilarial activity of the drug. Paras Immunol. 1997;19(4):191-5.
10. Abdel-Latif M, Sakran T. Detection for cross-reactive proteins in filarial worm Setaria equina, MCF-7 human breast cancer, and Huh-7 hepatoma cells. J Immunoas Immuochem. 2016;37(6):572-84.
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12. Mlcek J, Jurikova T, Skrovankova S, Sochor J. Quercetin and its anti-allergic immune response. Molecules. 2016;21(5):623.
13. Sozmen SC, Karaman M, Micili SC, Isik S, Bagriyanik A, Ayyildiz ZA, et al. Effects of quercetin treatment on epithelium-derived cytokines and epithelial cell apoptosis in allergic airway inflammation mice model. Iran J Allergy Asthma Immunol. 2016;15(6):487-97.
14. Flicker S, Linhart B, Wild C, Wiedermann U, Valenta R. Passive immunization with allergen-specific IgG antibodies for treatment and prevention of allergy. Immunobiol. 2013;218(6):884-91.
15. McKinney MM, Parkinson A. A simple, non-chromatographic procedure to purify immunoglobulins from serum and ascites fluid. J Immun Method. 1987;96(2):271-8.
16. Ravindran B, Satapathy A, Hussain T, Pattnaik A. Antibodies in human filariasis sera react with diethylcarbamazine. Clin Exp Immunol. 1989;78(2):219.
17. Galaly SR, Mohamed MA-H, Abdel-Latif M, Talaat RM. Rat Airway Changes after Challenge with Trimellitic Anhydride: Effect of Diethylcarbamazine Citrate and Omega-3 Fatty Acids. Egyptian Journal of Zoology. 2014;174(1324):1-46.
18. Bancroft JD, Gamble M. Theory and practice of histological techniques: Elsevier Health Sciences; 2008.
19. Park Y, Chang Y-S, Lee SW, Cho SY, Kim Y-K, Min K-U, et al. The enhanced effect of a hexameric deoxyriboguanosine run conjugation to CpG oligodeoxynucleotides on protection against allergic asthma. J Allergy Clin Immunol. 2001;108(4):570-6.
20. Cheng JB, Pillar JS, Shirley JT, Showell HJ, Watson JW, Cohan VL. Antigen-mediated pulmonary eosinophilia in immunoglobulin G1-sensitized guinea pigs: eosinophil peroxidase as a simple specific marker for detecting eosinophils in bronchoalveolar lavage fluid. J Pharmacol Exp Ther. 1993;264(2):922-9.
21. Abdel-Latif M, Sakran T, El-Shahawi G, El-Fayoumi H, El-Mallah AM. Immunomodulatory effect of diethylcarbamazine citrate plus filarial excretory-secretory product on rat hepatocarcinogenesis. Int Immunopharmacol. 2015;24(2):173-81.
22. Baba S, Kondo K, Toma-Hirano M, Kanaya K, Suzukawa K, Ushio M, et al. Local increase in IgE and class switch recombination to IgE in nasal polyps in chronic rhinosinusitis. Clin Exp Allergy. 2014;44(5):701-12.
23. Scheerens J, van Gessel SB, Nijkamp FP, Folkerts G. Eotaxin protein levels and airway pathology in a mouse model for allergic asthma. Eur J Pharmacol. 2002;453(1):111-7.
24. Kim SH, Hong JH, Lee YC. Chelidonine, a principal isoquinoline alkaloid of Chelidonium majus, attenuates eosinophilic airway inflammation by suppressing IL-4 and eotaxin-2 expression in asthmatic mice. Pharmacol Rep. 2015;67(6):1168-77.
25. Qian J, Ma X, Xun Y, Pan L. Protective effect of forsythiaside A on OVA-induced asthma in mice. Eur J Pharmacol. 2017;812:250-5.
26. Rosa SIG, Rios-Santos F, Balogun SO, de Almeida DAT, Damazo AS, da Cruz TCD, et al. Hydroethanolic extract from Echinodorus scaber Rataj leaves inhibits inflammation in ovalbumin-induced allergic asthma. J Ethnopharmacol. 2017;203:191-9.
27. Weg VB, Watson ML, Faccioli LH, Williams TJ. Investigation of the endogenous chemoattractants involved in 111In-eosinophil accumulation in passive cutaneous anaphylactic reactions in the guinea-pig. Br J Pharmacol. 1994;113(1):35-42.
28. de Silva R, Dasanayake W, Wickramasinhe GD, Karunatilake C, Weerasinghe N, Gunasekera P, et al. Sensitization to bovine serum albumin as a possible cause of allergic reactions to vaccines. Vaccine. 2017;35(11):1494-500.
29. Joskova M, Franova S, Sadlonova V. Acute bronchodilator effect of quercetin in experimental allergic asthma. Bratisl Lek Listy. 2011;112(1):9-12.
30. Moon H, Choi HH, Lee JY, Moon HJ, Sim SS, Kim CJ. Quercetin inhalation inhibits the asthmatic responses by exposure to aerosolized-ovalbumin in conscious guinea-pigs. Arch Pharm Res. 2008;31(6):771-8.
31. Farrar MA, Schreiber RD. The molecular cell biology of interferon-gamma and its receptor. Ann Rev Immunol. 1993;11(1):571-611.
32. Renauld J-C. New insights into the role of cytokines in asthma. J Clin Path. 2001;54(8):577-89.
33. Teixeira LK, Fonseca BP, Barboza BA, Viola JP. The role of interferon-gamma on immune and allergic responses. Memórias do Instituto Oswaldo Cruz. 2005;100:137-44.
34. Pernis A, Gupta S, Gollob KJ, Garfein E, Coffman RL, Schindler C, et al. Lack of interferon gamma receptor beta chain and the prevention of interferon gamma signaling in TH1 cells. Science. 1995;269(5221):245-7.
35. Bocek Jr P, Foucras G, Paul WE. Interferon γ enhances both in vitro and in vivo priming of CD4+ T cells for IL-4 production. J Exp Med. 2004;199(12):1619-30.
36. Refaeli Y, Van Parijs L, Alexander SI, Abbas AK. Interferon γ is required for activation-induced death of T lymphocytes. J Exp Med. 2002;196(7):999-1005.
37. Kodama T, Kuribayashi K, Nakamura H, Fujita M, Fujita T, Takeda K, et al. Role of interleukin-12 in the regulation of CD4+ T cell apoptosis in a mouse model of asthma. Clin Exp Immunol. 2003;131(2):199-205.
38. Sak K, Kasemaa K, Everaus H. Potentiation of luteolin cytotoxicity by flavonols fisetin and quercetin in human chronic lymphocytic leukemia cell lines. Food Funct. 2016;7(9):3815-24.
39. Chan GKL, Hu WWH, Zheng ZX, Huang M, Lin YXY, Wang CY, et al. Quercetin Potentiates the NGF-Induced Effects in Cultured PC 12 Cells: Identification by HerboChips Showing a Binding with NGF. Hindawi. 2018;2018:1502457.
40. Park HJ, Lee CM, Jung ID, Lee JS, Jeong YI, Chang JH, et al. Quercetin regulates Th1/Th2 balance in a murine model of asthma. Int Immunopharmacol. 2009;9(3):261-7.
2. Maltby S, Tay HL, Yang M, Foster PS. Mouse models of severe asthma: U nderstanding the mechanisms of steroid resistance, tissue remodelling and disease exacerbation. Respirol. 2017;22(5):874-85.
3. Acharya KR, Ackerman SJ. Eosinophil granule proteins: form and function. J Biol Chem. 2014;289(25):17406-15.
4. Corry DB, Grünig G, Hadeiba H, Kurup VP, Warnock ML, Sheppard D, et al. Requirements for allergen-induced airway hyperreactivity in T and B cell-deficient mice. Mol Med.1998;4(5):344-55.
5. Barnes PJ. Glucocorticosteroids: current and future directions. Brit J Pharm.2011;163(1):29-43.
6. Masid-de-Brito D, Queto T, Gaspar-Elsas MIC, Xavier-Elsas P. Roles of 5-lipoxygenase and cysteinyl-leukotriene type 1 receptors in the hematological response to allergen challenge and its prevention by diethylcarbamazine in a murine model of asthma. Med Inflam. 2014;2014.
7. Florêncio M, Saraiva K, Peixoto C. The effects of diethylcarbamazine on the ultrastructure of lung cells in vivo. Tiss Cell. 2005;37(3):241-6.
8. Tsanglao WR, Nandan D, Chandelia S, Arya NK, Sharma A. Filarial tropical pulmonary eosinophilia: a condition masquerading asthma, a series of 12 cases. J Asthma.2019;56(7):791-8.
9. Mukhopadhyay S, Ravindran B. Antibodies to diethylcarbamazine potentiate the antifilarial activity of the drug. Paras Immunol. 1997;19(4):191-5.
10. Abdel-Latif M, Sakran T. Detection for cross-reactive proteins in filarial worm Setaria equina, MCF-7 human breast cancer, and Huh-7 hepatoma cells. J Immunoas Immuochem. 2016;37(6):572-84.
11. Gupta K, Kumar S, Gupta RK, Sharma A, Verma AK, Stalin K, et al. Reversion of asthmatic complications and mast cell signalling pathways in BALB/c mice model using quercetin nanocrystals. J Biomed Nanotech. 2016;12(4):717-31.
12. Mlcek J, Jurikova T, Skrovankova S, Sochor J. Quercetin and its anti-allergic immune response. Molecules. 2016;21(5):623.
13. Sozmen SC, Karaman M, Micili SC, Isik S, Bagriyanik A, Ayyildiz ZA, et al. Effects of quercetin treatment on epithelium-derived cytokines and epithelial cell apoptosis in allergic airway inflammation mice model. Iran J Allergy Asthma Immunol. 2016;15(6):487-97.
14. Flicker S, Linhart B, Wild C, Wiedermann U, Valenta R. Passive immunization with allergen-specific IgG antibodies for treatment and prevention of allergy. Immunobiol. 2013;218(6):884-91.
15. McKinney MM, Parkinson A. A simple, non-chromatographic procedure to purify immunoglobulins from serum and ascites fluid. J Immun Method. 1987;96(2):271-8.
16. Ravindran B, Satapathy A, Hussain T, Pattnaik A. Antibodies in human filariasis sera react with diethylcarbamazine. Clin Exp Immunol. 1989;78(2):219.
17. Galaly SR, Mohamed MA-H, Abdel-Latif M, Talaat RM. Rat Airway Changes after Challenge with Trimellitic Anhydride: Effect of Diethylcarbamazine Citrate and Omega-3 Fatty Acids. Egyptian Journal of Zoology. 2014;174(1324):1-46.
18. Bancroft JD, Gamble M. Theory and practice of histological techniques: Elsevier Health Sciences; 2008.
19. Park Y, Chang Y-S, Lee SW, Cho SY, Kim Y-K, Min K-U, et al. The enhanced effect of a hexameric deoxyriboguanosine run conjugation to CpG oligodeoxynucleotides on protection against allergic asthma. J Allergy Clin Immunol. 2001;108(4):570-6.
20. Cheng JB, Pillar JS, Shirley JT, Showell HJ, Watson JW, Cohan VL. Antigen-mediated pulmonary eosinophilia in immunoglobulin G1-sensitized guinea pigs: eosinophil peroxidase as a simple specific marker for detecting eosinophils in bronchoalveolar lavage fluid. J Pharmacol Exp Ther. 1993;264(2):922-9.
21. Abdel-Latif M, Sakran T, El-Shahawi G, El-Fayoumi H, El-Mallah AM. Immunomodulatory effect of diethylcarbamazine citrate plus filarial excretory-secretory product on rat hepatocarcinogenesis. Int Immunopharmacol. 2015;24(2):173-81.
22. Baba S, Kondo K, Toma-Hirano M, Kanaya K, Suzukawa K, Ushio M, et al. Local increase in IgE and class switch recombination to IgE in nasal polyps in chronic rhinosinusitis. Clin Exp Allergy. 2014;44(5):701-12.
23. Scheerens J, van Gessel SB, Nijkamp FP, Folkerts G. Eotaxin protein levels and airway pathology in a mouse model for allergic asthma. Eur J Pharmacol. 2002;453(1):111-7.
24. Kim SH, Hong JH, Lee YC. Chelidonine, a principal isoquinoline alkaloid of Chelidonium majus, attenuates eosinophilic airway inflammation by suppressing IL-4 and eotaxin-2 expression in asthmatic mice. Pharmacol Rep. 2015;67(6):1168-77.
25. Qian J, Ma X, Xun Y, Pan L. Protective effect of forsythiaside A on OVA-induced asthma in mice. Eur J Pharmacol. 2017;812:250-5.
26. Rosa SIG, Rios-Santos F, Balogun SO, de Almeida DAT, Damazo AS, da Cruz TCD, et al. Hydroethanolic extract from Echinodorus scaber Rataj leaves inhibits inflammation in ovalbumin-induced allergic asthma. J Ethnopharmacol. 2017;203:191-9.
27. Weg VB, Watson ML, Faccioli LH, Williams TJ. Investigation of the endogenous chemoattractants involved in 111In-eosinophil accumulation in passive cutaneous anaphylactic reactions in the guinea-pig. Br J Pharmacol. 1994;113(1):35-42.
28. de Silva R, Dasanayake W, Wickramasinhe GD, Karunatilake C, Weerasinghe N, Gunasekera P, et al. Sensitization to bovine serum albumin as a possible cause of allergic reactions to vaccines. Vaccine. 2017;35(11):1494-500.
29. Joskova M, Franova S, Sadlonova V. Acute bronchodilator effect of quercetin in experimental allergic asthma. Bratisl Lek Listy. 2011;112(1):9-12.
30. Moon H, Choi HH, Lee JY, Moon HJ, Sim SS, Kim CJ. Quercetin inhalation inhibits the asthmatic responses by exposure to aerosolized-ovalbumin in conscious guinea-pigs. Arch Pharm Res. 2008;31(6):771-8.
31. Farrar MA, Schreiber RD. The molecular cell biology of interferon-gamma and its receptor. Ann Rev Immunol. 1993;11(1):571-611.
32. Renauld J-C. New insights into the role of cytokines in asthma. J Clin Path. 2001;54(8):577-89.
33. Teixeira LK, Fonseca BP, Barboza BA, Viola JP. The role of interferon-gamma on immune and allergic responses. Memórias do Instituto Oswaldo Cruz. 2005;100:137-44.
34. Pernis A, Gupta S, Gollob KJ, Garfein E, Coffman RL, Schindler C, et al. Lack of interferon gamma receptor beta chain and the prevention of interferon gamma signaling in TH1 cells. Science. 1995;269(5221):245-7.
35. Bocek Jr P, Foucras G, Paul WE. Interferon γ enhances both in vitro and in vivo priming of CD4+ T cells for IL-4 production. J Exp Med. 2004;199(12):1619-30.
36. Refaeli Y, Van Parijs L, Alexander SI, Abbas AK. Interferon γ is required for activation-induced death of T lymphocytes. J Exp Med. 2002;196(7):999-1005.
37. Kodama T, Kuribayashi K, Nakamura H, Fujita M, Fujita T, Takeda K, et al. Role of interleukin-12 in the regulation of CD4+ T cell apoptosis in a mouse model of asthma. Clin Exp Immunol. 2003;131(2):199-205.
38. Sak K, Kasemaa K, Everaus H. Potentiation of luteolin cytotoxicity by flavonols fisetin and quercetin in human chronic lymphocytic leukemia cell lines. Food Funct. 2016;7(9):3815-24.
39. Chan GKL, Hu WWH, Zheng ZX, Huang M, Lin YXY, Wang CY, et al. Quercetin Potentiates the NGF-Induced Effects in Cultured PC 12 Cells: Identification by HerboChips Showing a Binding with NGF. Hindawi. 2018;2018:1502457.
40. Park HJ, Lee CM, Jung ID, Lee JS, Jeong YI, Chang JH, et al. Quercetin regulates Th1/Th2 balance in a murine model of asthma. Int Immunopharmacol. 2009;9(3):261-7.
| Files | ||
| Issue | Vol 19 No 4 (2020) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijaai.v19i4.4112 | |
| PMID | 33463104 | |
| Keywords | ||
| Antibodies Anti-allergic agents Asthma Diethylcarbamazine Quercetin | ||
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How to Cite
1.
Abdul-Razek N, M. El-Mallah A-M, Abuelsaad A, Abdel-Latif M. Enhancement of Anti-allergic Effect of Diethylcarbamazine Citrate in Asthmatic Mouse Model: Testing of Anti-drug Antibodies and Quercetin. Iran J Allergy Asthma Immunol. 2020;19(4):373-385.
