Evaluating the Immunoreactivity of Ailanthus Altissima (The Tree of Heaven) Pollen Extract in Atopic Patients
IgE-mediated hypersensitivity reaction to pollens is a common health problem in atopic patients. In this regard, the assessment of the allergenicity of highly pollinating plants would be demanding. Based on the increment of Ailanthus altissima (A. altissima) tree in some parts of Iran and considering its probable role in respiratory allergy, in this study, we aimed to investigate its IgE-immunoreactivity and in diagnostic applications. One hundred and twenty-five allergic rhinitis patients who were diagnosed as high IgE responders and demonstrated seasonal rhinitis or rhinoconjunctivitis, as well as 20 healthy controls (HCs) with no allergic symptoms, were enrolled in this study. Total protein extract was prepared from A. altissima pollens and subjected to quality control experiments and finally used in ELISA and western blotting studies. Approximately 24% of the atopic patients (30 from 125) showed positive immunoreactivity to A. altissima extract. The median (IQR) of absorbance (450 nm) of the specific IgE against A. altissima pollen extract in HCs and positive groups were 0.33 (0.28-0.42) and 0.59 (0.36-0.79), respectively (p<0.001). Receiver operating characteristics (ROC) curve analysis of the specific ELISA results, revealed a cut-off value of 0.46 and a sensitivity of 70% and specificity of 100%. Western blotting with the sera positive cases revealed that the main immunoreactive proteins range from 10 to 70 kDa. This study revealed that some of A. altissima pollen proteins ranging from 10 to 70 kDa show IgE-reactivity in atopic patients and may play a role in their allergic reaction symptoms.
2. Mohammadi M, Mokhtarian K, Kardar GA, Farrokhi S, Sadroddiny E, Khorramizadeh MR, et al. Expression of recombinant parvalbumin from wolf-herring fish and determination of its IgE-binding capability. Food Agr Immunol. 2017;28(4):573-85.
3. Moghaddam MV, Fallahpour M, Mohammadi M,
Varaee FR, Mokhtarian K, Khoshmirsafa M, et al. Identification of polcalcin as a novel allergen of Amaranthus retroflexus pollen. Allergol Immunopathol (Madr) 2019; 47(4):357-364.
4. Bidad K, Nicknam MH, Farid R. A review of allergy and allergen specific immunotherapy. Iran J Allergy Asthma. 2011;10(1):1-9.
5. Newell JM. A review of chemical studies on the allergens in pollens. Journal of Allergy. 1942;13(2):177-203.
6. Weber RW. Cross-reactivity of pollen allergens: recommendations for immunotherapy vaccines. Cure Opin Allergy CL. 2005;5(6):563-9.
7. Sladonja B, Susek M, Guillermic J. Review on Invasive Tree of Heaven (Ailanthus altissima (Mill.) Swingle) Conflicting Values: Assessment of Its Ecosystem Services and Potential Biological Threat. Environ Manage. 2015;56(4):1009-34.
8. Ballero M, Ariu A, Falagiani Piu P, G. Allergy to Ailanthus altissima (tree of heaven) pollen. Allergy. 2003;58(6):532-3.
9. Tas J. Ailanthus glandulosa pollen as a cause of hay fever. Ann Allergy. 1956;14(1):47-8.
10. Whiticar R, Harvey C. Beware the tree of heaven! (<em>Ailanthus altissima</em>). Emerg Med J. 2009;26(6):451.
11. Mousavi F, Majd A, Shahali Y, Ghahremaninejad F, Kardar G, Pourpak Z. Pollinosis to tree of heaven (Ailanthus altissima) and detection of allergenic proteins: a case report. Ann Allergy Asthma Im. 2016;116(4):374-5.
12. Mousavi F, Majd A, Shahali Y, Ghahremaninejad F, Shokouhi Shoormasti R, Pourpak Z. Immunoproteomics of tree of heaven (Ailanthus atltissima) pollen allergens. J PROTEOMICS. 2017;154:94-101.
13. Bennett WO, Paget JT, Mackenzie D. Surgery for a tree surgeon? Acute presentation of contact dermatitis due to Ailanthus altissima. J Plast Reconstr Aes:2013;66(3):e79-80.
14. Wang R, Lu Y, Li H, Sun L, Yang N, Zhao M, et al. Antitumor activity of the Ailanthus altissima bark phytochemical ailanthone against breast cancer MCF-7 cells. Oncol Lett. 2018;15(4):6022-8.
15. Galli SJ, Tsai M, Piliponsky AM. The development of allergic inflammation. Nature. 2008;454(7203):445-54.
16. Miller JH. Ailanthus altissima (Mill.) Swingle ailanthus. Silvics of North America. 1990;2:101-4.
17. Kim HM, Lee JS, Sezirahiga J, et al. A New Canthinone-Type Alkaloid Isolated from Ailanthus altissima Swingle. Molecules. 2016;21(5).
18. Cho SK, Jeong M, Jang DS, Choi JH. Anti-inflammatory Effects of Canthin-6-one Alkaloids from Ailanthus altissima. Planta Med. 2018;84(8):527-35.
19. Kumar D, Bhujbal SS, Deoda RS, Mudgade SC. Bronchodilator activity of aqueous extract of stem bark of Ailanthus excelsa Roxb. Pharmacogn Res.2010;2(2):102-6.
20. Kumar D, Bhat ZA, Singh P, Bhujbal SS, Deoda RS. Antihistaminic activity of aqueous extract of stem bark of Ailanthus excelsa Roxb. Pharmacogn Res.2011;3(3):220-4.
21. Kang TH, Choi IY, Kim SJ, Moon PD, et al. Ailanthus altissima swingle has anti-anaphylactic effect and inhibits inflammatory cytokine expression via suppression of nuclear factor-kappaB activation. In Vitro Cell Dev Biol Anim. 2010;46(1):72-81.
22. Jin M, Yang JH, Lee E, Lu Y, Kwon S, Son KH, et al. Antiasthmatic activity of luteolin-7-O-glucoside from Ailanthus altissima through the downregulation of T helper 2 cytokine expression and inhibition of prostaglandin E2 production in an ovalbumin-induced asthma model. Biol Pharm Bull. 2009;32(9):1500-3.
23. Yan Z-Y, Chen J-J, Duan Z-K, Yao G-D, Lin B, Wang X-B, et al. Racemic phenylpropanoids from the root barks of Ailanthus altissima (Mill.) Swingle with cytotoxicity against hepatoma cells. Fitoterapia. 2018;130:234-40.
24. Wang R, Lu Y, Li H, Sun L, Yang N, Zhao M, et al. Antitumor activity of the Ailanthus altissima bark phytochemical ailanthone against breast cancer MCF-7 cells. Oncol Lett. 2018;15(4):6022-8.
25. Cho S-K, Jeong M, Jang DS, Choi J-H. Anti-inflammatory Effects of Canthin-6-one Alkaloids from Ailanthus altissima. Planta Med. 2018;50(08):527-35.
26. Zhao C-C, Shao J-H, Li X, Xu J, Zhang P. Antimicrobial constituents from fruits ofAilanthus altissima swingle. Arch Pharm Res. 2005;28(10):1147-51.
27. Pedersini C, Bergamin M, Aroulmoji V, Baldini S, Picchio R, Pesce PG, et al. Herbicide activity of extracts from Ailanthus altissima (Simaroubaceae). Nat Prod Commun. 2011;6(5):593-6.
|Issue||Vol 19, No 2 (2020)|
|Allergy Atopy Ailanthus altissima Enzyme-linked immunosorbent assay (ELISA) IgE-reactivity Western blotting|
|Rights and permissions|
|This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.|