Anti-inflammatory Effects of Matricaria chamomilla Extracts on BALB/c Mice Macrophages and Lymphocytes

  • Zahra Asadi Department of Biochemistry, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
  • Tooba Ghazanfari Mail Immunoregulation Research Center, Shahed University, Tehran, Iran
  • Helia Hatami Immunoregulation Research Center, Shahed University, Tehran, Iran
Keywords:
Apigenin, Lymphocytes, Matricaria chamomilla, Macrophages, Th1-Th2 balance

Abstract

Matricaria chamomilla (MC) was shown to have anti-inflammatory effects. Flavonoids are major groups of MC immunomodulators. The anti-inflammatory effects of apigenin as an MC flavonoid has already been demonstrated.
In this study, we aimed to report the amount of this compound by liquid chromatography-mass spectrometry (LC-MS) and measuring the total phenol content (TPC) in both the MC aqueous and alcoholic extracts. We also investigated the MC aqueous and ethanolic extracts effect on BALB/c separated macrophages and lymphocytes cell viability and macrophage nitric oxide production. Interferon-γ and interleukin-10 secretion were also measured in lymphocytes.
We found that the amount of apigenin was 0.078 and 0.25 mg/g per each of dry aqueous and alcoholic extracts, respectively. Also, the total phenol content was 2.99% in aqueous and 3.95% in alcoholic extracts. BALB/c separated macrophages cell viability significantly increased when treated with the MC aqueous extract but decreased when treated by the MC alcoholic extract in the presence of lipopolysaccharide. Also, the amount of nitric oxide production by macrophages and BALB/c separated lymphocytes cell viability in treatment with aqueous and alcoholic extracts significantly decreased. Interferon-γ increased, and interleukin-10 decreased in lymphocytes treated with the MC aqueous extract, which may suggest Th1 polarization. There was no significant change in the interferon-γ level in lymphocytes when treated with the MC alcoholic extract, but the level of IL-10 increased in these cells.
Altogether, besides the anti-inflammatory effect of MC extracts, we found MC aqueous extract effects as disrupting Th1/Th2 balance to Th1 upregulation. Overall, the anti-inflammatory effect of the MC alcoholic extract was higher than the MC aqueous extract.

References

1. Lebish IJ, Moraski RM. Mechanisms of immunomodulation by drugs. Toxicol Pathol 1987; 15(3):338-45.
2. Pan SY, Zhou SF, Gao SH, Yu ZL, Zhang SF, Tang MK, et al. New Perspectives on How to Discover Drugs from Herbal Medicines: CAM's Outstanding Contribution to Modern Therapeutics. Evid Based Complement Alternat Med 2013;627375.
3. McKay DL, Blumberg JB. A review of the bioactivity and potential health benefits of peppermint tea (Mentha piperita L.). Phytother Res 2006; 20(8):619-33.
4. Longo R, São Dimas J. Effects of Chamomilla recutita (L.) on oral wound healing in rats. Cir Bucal 2011; 16(6):716-21.
5. Wu Y-n, Xu Y, Yao L. Anti-inflammatory and anti-allergic effects of German chamomile (Matricaria chamomilla L.). J Ethnopharmacol 2012; 15(1):75-83.
6. Singh O, Khanam Z, Misra N, Srivastava MK. Chamomile (Matricaria chamomilla L.): an overview. Pharmacogn Rev 2011; 5(9):82-95.
7. Srivastava JK, Gupta S. Extraction, characterization, stability and biological activity of flavonoids isolated from chamomile flowers. Mol Cell Pharmacol 2009; 1(3):138.
8. Fonseca FN, Tavares MF, Horváth C. Capillary electrochromatography of selected phenolic compounds of Chamomilla recutita. J Chromatogr A 2007; 1154(1): 390-399.
9. Bellik Y, Boukraâ L, Alzahrani H, Bakhotmah B, Abdellah F, Hammoudi S, et al. Molecular mechanism underlying anti-inflammatory and anti-allergic activities of phytochemicals: an update. Molecules 2013; 18(1):322-53.
10. Liang Y-C, Huang Y-T, Tsai S-H, Lin-Shiau S-Y, Chen C-F, Lin J-K. Suppression of inducible cyclooxygenase and inducible nitric oxide synthase by apigenin and related flavonoids in mouse macrophages. Carcinogenesis 1999; 20(10):1945-52.
11. Kawai M, Hirano T, Higa S, Arimitsu J, Maruta M, Kuwahara Y, et al. Flavonoids and related compounds as anti-allergic substances. Allergol Int 2007; 56(2):113-23.
12. Li J, Zhang B. Apigenin protects ovalbumin-induced asthma through the regulation of Th17 cells. Fitoterapia 2013; 91:298-304.
13. Drummond EM, Harbourne N, Marete E, Martyn D, Jacquier J, O'riordan D, et al. Inhibition of proinflammatory biomarkers in THP1 macrophages by polyphenols derived from chamomile, meadowsweet and willow bark. Phytother Res 2013; 27(4):588-94.
14. Pang L, Li R, Zhou L. Inhibitory effects of apigenin on the expression of GATA-3 and Th2 cytokines in asthmatic mice. Zhongguo Zhong Xi Yi Jie He Za Zhi 2010; 30(4):383-7.
15. Esmaeilzadeh Kenari R, Mohsenzadeh F, Amiri ZR. Antioxidant activity and total phenolic compounds of Dezful sesame cake extracts obtained by classical and ultrasound-assisted extraction methods. Food Sci Nutr 2014; 2(4):426-35.
16. Bhaskaran N, Shukla S, Srivastava JK, Gupta S. Chamomile: an anti-inflammatory agent inhibits inducible nitric oxide synthase expression by blocking RelA/p65 activity. Int J Mol Med 2010; 26(6):935-40.
17. A W. Folin-Ciocalteau Micro Method for Total Phenol in Wine: University of California, Davis; [Available from: https://waterhouse.ucdavis.edu/folin-ciocalteau-micro-method-total-phenol-wine.
18. Zhang X, Goncalves R, Mosser DM. The isolation and characterization of murine macrophages. Curr Protoc Immunol 2008; Chapter 14:Unit-14.1.
19. Lim JF, Berger H, Su IH. Isolation and Activation of Murine Lymphocytes. J Vis Exp 2016; (116):54596.
20. Sun J, Zhang X, Broderick M, Fein H. Measurement of nitric oxide production in biological systems by using Griess reaction assay. Sensors 2003; 3(8):276-84.
21. Haghi G, Hatami A, Safaei A, Mehran M. Analysis of phenolic compounds in Matricaria chamomilla and its extracts by UPLC-UV. Res Pharm Sci 2014; 9(1):31-7.
22. Srivastava JK, Shankar E, Gupta S. Chamomile: a herbal medicine of the past with a bright future. Mol Med Rep 2010; 3(6):895-901.
23. Lovrić V, Putnik P, Kovačević DB, Jukić M, Dragović-Uzelac V. Effect of Microwave-Assisted Extraction on the Phenolic Compounds and Antioxidant Capacity of Blackthorn Flowers. Food Technol Biotechnol. 2017;55(2):243–250.
24. Verbeek R, van Tol EA, van Noort JM. Oral flavonoids delay recovery from experimental autoimmune encephalomyelitis in SJL mice. Biochem Pharmacol 2005; 70(2):220-8.
25. Ogata I, Kawanai T, Hashimoto E, Nishimura Y, Oyama Y, Seo H. Bisabololoxide A, one of the main constituents in German chamomile extract, induces apoptosis in rat thymocytes. Arch Toxicol 2010; 84(1):45.
26. Zhang S, Liu X, Sun C, Yang J, Wang L, Liu J, et al. Apigenin attenuates experimental autoimmune myocarditis by modulating Th1/Th2 cytokine balance in mice. Inflammation 2016; 39(2):678-86.
27. Trinchieri G. Interleukin-10 production by effector T cells: Th1 cells show self control. J Exp Med. 2007;204(2):239–243.
28. Verbeek R, Plomp AC, van Tol EA, van Noort JM. The flavones luteolin and apigenin inhibit in vitro antigen-specific proliferation and interferon-gamma production by murine and human autoimmune T cells. Biochem Pharmacol 2004; 68(4):621-9.
29. Yano S, Umeda D, Yamashita S, Yamada K, Tachibana H. Dietary apigenin attenuates the development of atopic dermatitis-like skin lesions in NC/Nga mice. J Nutr Biochem 2009; 20(11):876-81.
Published
2020-05-17
How to Cite
1.
Asadi Z, Ghazanfari T, Hatami H. Anti-inflammatory Effects of Matricaria chamomilla Extracts on BALB/c Mice Macrophages and Lymphocytes. Iran J Allergy Asthma Immunol. 19(S1):63-73.
Section
Original Article(s)