Induction of Immune Response and Protective Immunity by a Local Isolated Varicella Virus in Animal Model: A Future Candidates for Vaccine Production
Abstract
Preparation of the indigenous varicella zoster vaccine could significantly reduce the disease burden of varicella zoster virus especially in immunosuppressed children. To achieve this goal, the varicella zoster virus was isolated from an 8 years boy infected with chicken pox. The virus was cultivated in sensitive cell line and determined varicella zoster. The adaptation and attenuation of virus was carried out after several passages in MRC-5 cell culture, Primary Guinea pig embryo fibroblast cell culture and again switching in MRC-5 cell culture. The challenged of vaccine dose was found 3LogCCID50. Following two doses of immunization in guinea pigs via inoculated cell culture-fluid attenuated- local isolated VZV at zero and 14 day, the humoral immune response, varicella-zoster virus (VZV) IgG and IgM were determined using enzyme-linked Immunosorbent and seroneutralization assays at 7, 14, 21, 30, 60, 90.120 days after receiving of the first and second dose of vaccine. The results of immunization showed good 93% seroconversion in guinea pig which compared with vOKa vaccine was not significant (p<0.05). The prepared attenuate varicella zoster virus promising a candidate Virus for our future plan to vaccine production.
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2. Katzoli P, Sakellaris G, Ergazaki M, Charissis G, Spandidos DA, Sourvinos G. Detection of herpes viruses in children with acute appendicitis. J Clin Virol 2009; 44(4):282-6.
3. Tan AY, Connett CJ, Connet GJ, Quek SC, Yap HK, Meurice F, et al. Use of a reformulated Oka strain varicella vaccine (SmithKline Beecham Biologicals/Oka) in healthy children. Eur J Pediatr 1996; 155(8):706–11.
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7. Ozaki T, Asano Y .Development of varicella vaccine in Japan and future prospects. Vaccine 2016; 34(29):3427-33.
8. Safarnezhad Tameshkel F, Karbalaie Niya MH, Keyvani H. Enzymatic Digestion Pattern of Varicella Zoster Virus ORF38 and ORF54 in Chickenpox Patients Using RFLP Technique. Iran J Pathol 2016; 11(1):35 – 40.
9. Amjadi O, Rafiei A, Haghshenas M, Navaei RA, Valadan R, Hosseini-Khah Z, et al. A systematic review and meta-analysis of Seroprevalence of Varicella Zoster virus: A nationwide population-based study. J Clin Virol 2017; 87:49-59
10. Mohammadi A, Farzinpoor M, Lotfi M, Ghorbani R. Comparative Sensitivity Analysis Between two methods for Species Differentiation and Interspecies Cross Contamination in Animal Cell culture. Arch Med Lab Sci 2016; 2(3):84-8
11. Cohen, JI; Straus, SE. Arvin, A. Varicella-Zoster Virus: Replication, Pathogenesis, and Management. In: Knipe, DM. Howley, PM., editors. Fields Virology. 5. Lippincott Williams and Wilkins; Philadelphia: 2007. p. 2773-2818
12. Abbas Shafyi, Ashraf Mohammadi, Measles Vaccines in Iran: A 50-Year Review of Vaccine Development, Production and Effectiveness (1967 - 2017) Jundishapour Journal of Microbiology. 2018. DOI: 10.5812/jjm.60725.
13. Sauerbrei A, Eichhorn U, Schacke M, Wutzler P. Laboratory diagnosis of herpes zoster. J Clin Virol 1999; 14(1):31-6.
14. Gershon AA. Varicella zoster vaccines and their implications for development of HSV vaccines. Virology 2013; 435(1):29–36.
15. LaRussa P, Steinberg SP, Seeman MD, Gershon AA. Determination of immunity to varicella-zoster virus by means of an intradermal skin test. J Infect Dis 1985; 152(5):869-75.
16. Baba K, Shiraki K, Kanesaki T, Yamanishi K, Ogra PL, Yabuuchi H, et al. Specificity of skin test with varicella-zoster virus antigen in varicella-zoster and herpes simplex virus infections. J Clin Microbiol 1987; 25(11):193-6.
17. Shrim A, Koren G, Yudin MH, Farine D. No. 274-Management of Varicella Infection (Chickenpox) in Pregnancy. J Obstet Gynaecol Can 2018; 48(8):e652-7.
18. Sauerbrei A, Wutzler P. The congenital varicella syndrome. J. Perinatal 2000; 20(8 pt1):548–54.
19. Critselis, E., Nastos, P., Theodoridou, K., Theodoridou, M., Tsolia, M. Paevangelou, V. (2012b) Association between variations in the epidemiology of varicella infection and climate change in a temperate region. In 30th Annual Meeting of the European Society for Pediatric Infectious Diseases, 8–12 May 2012, Thessaloniki, Greece
20. Haberthur K, Messaoudi I. Animal models of varicella zoster virus infection. Pathogens 2013; 2(2):364-82.
21. Fatemeh Esna-Ashari, Farzaneh Sabahi, Mehrdad Ravanshad, Ashraf Mohammadi. Isolation and characterization and Attenuation of local Varicella virus from a symptomatic Infection; A future varicella virus candidate. Archive of Razi (Publish in press)
22.Loparev VN, Rubtcova EN, Bostik V, Govil D, Birch CJ, Druce JD, et al. Identification of five major and two minor genotypes of varicella-zoster virus strains: a practical two-amplicon approach used to genotype clinical isolates in Australia and New Zealand. J Virol 2007; 81(23):12758-65.
23. Liu J, Wang M, Gan L, Yang S, Chen J. Genotyping of clinical varicella zoster virus isolates collected in China. J Clin Microbial 2009; 47(5):1418-23.
24.Gomi Y, Sunamachi H, Mori Y, Nagaike K, Takahashi M, Yamanishi K. Comparison of the komplete DNA sequences of the Oka varicella vaccine and its parentel virus. J Virol 2002; 76(22):11447-59.
2. Katzoli P, Sakellaris G, Ergazaki M, Charissis G, Spandidos DA, Sourvinos G. Detection of herpes viruses in children with acute appendicitis. J Clin Virol 2009; 44(4):282-6.
3. Tan AY, Connett CJ, Connet GJ, Quek SC, Yap HK, Meurice F, et al. Use of a reformulated Oka strain varicella vaccine (SmithKline Beecham Biologicals/Oka) in healthy children. Eur J Pediatr 1996; 155(8):706–11.
4. Levin MJ, Varicella vaccine. In: Plotkins S, Orenstein W, Offit PA, Edwards KM. Vaccines, 6th ed. Philadelphia, PA: Elsevier; 2018.p.1268- 1282
5. WHO Requirements for measles, mumps and rubella vaccines and combined vaccine (live), WHO Tech. Rep. Ser. 840 (1994) 100-176.TRS VZV.
6. Sessani, A. Mirchamsy, H. Shafyi, A. Ahourai, P. Razavi, J. Gholami, A. Mohammadi, A. Development a New Live Attenuated Mumps Vaccine in Human Diploid Cells. Biologicals 1991; 19(3):203-11.
7. Ozaki T, Asano Y .Development of varicella vaccine in Japan and future prospects. Vaccine 2016; 34(29):3427-33.
8. Safarnezhad Tameshkel F, Karbalaie Niya MH, Keyvani H. Enzymatic Digestion Pattern of Varicella Zoster Virus ORF38 and ORF54 in Chickenpox Patients Using RFLP Technique. Iran J Pathol 2016; 11(1):35 – 40.
9. Amjadi O, Rafiei A, Haghshenas M, Navaei RA, Valadan R, Hosseini-Khah Z, et al. A systematic review and meta-analysis of Seroprevalence of Varicella Zoster virus: A nationwide population-based study. J Clin Virol 2017; 87:49-59
10. Mohammadi A, Farzinpoor M, Lotfi M, Ghorbani R. Comparative Sensitivity Analysis Between two methods for Species Differentiation and Interspecies Cross Contamination in Animal Cell culture. Arch Med Lab Sci 2016; 2(3):84-8
11. Cohen, JI; Straus, SE. Arvin, A. Varicella-Zoster Virus: Replication, Pathogenesis, and Management. In: Knipe, DM. Howley, PM., editors. Fields Virology. 5. Lippincott Williams and Wilkins; Philadelphia: 2007. p. 2773-2818
12. Abbas Shafyi, Ashraf Mohammadi, Measles Vaccines in Iran: A 50-Year Review of Vaccine Development, Production and Effectiveness (1967 - 2017) Jundishapour Journal of Microbiology. 2018. DOI: 10.5812/jjm.60725.
13. Sauerbrei A, Eichhorn U, Schacke M, Wutzler P. Laboratory diagnosis of herpes zoster. J Clin Virol 1999; 14(1):31-6.
14. Gershon AA. Varicella zoster vaccines and their implications for development of HSV vaccines. Virology 2013; 435(1):29–36.
15. LaRussa P, Steinberg SP, Seeman MD, Gershon AA. Determination of immunity to varicella-zoster virus by means of an intradermal skin test. J Infect Dis 1985; 152(5):869-75.
16. Baba K, Shiraki K, Kanesaki T, Yamanishi K, Ogra PL, Yabuuchi H, et al. Specificity of skin test with varicella-zoster virus antigen in varicella-zoster and herpes simplex virus infections. J Clin Microbiol 1987; 25(11):193-6.
17. Shrim A, Koren G, Yudin MH, Farine D. No. 274-Management of Varicella Infection (Chickenpox) in Pregnancy. J Obstet Gynaecol Can 2018; 48(8):e652-7.
18. Sauerbrei A, Wutzler P. The congenital varicella syndrome. J. Perinatal 2000; 20(8 pt1):548–54.
19. Critselis, E., Nastos, P., Theodoridou, K., Theodoridou, M., Tsolia, M. Paevangelou, V. (2012b) Association between variations in the epidemiology of varicella infection and climate change in a temperate region. In 30th Annual Meeting of the European Society for Pediatric Infectious Diseases, 8–12 May 2012, Thessaloniki, Greece
20. Haberthur K, Messaoudi I. Animal models of varicella zoster virus infection. Pathogens 2013; 2(2):364-82.
21. Fatemeh Esna-Ashari, Farzaneh Sabahi, Mehrdad Ravanshad, Ashraf Mohammadi. Isolation and characterization and Attenuation of local Varicella virus from a symptomatic Infection; A future varicella virus candidate. Archive of Razi (Publish in press)
22.Loparev VN, Rubtcova EN, Bostik V, Govil D, Birch CJ, Druce JD, et al. Identification of five major and two minor genotypes of varicella-zoster virus strains: a practical two-amplicon approach used to genotype clinical isolates in Australia and New Zealand. J Virol 2007; 81(23):12758-65.
23. Liu J, Wang M, Gan L, Yang S, Chen J. Genotyping of clinical varicella zoster virus isolates collected in China. J Clin Microbial 2009; 47(5):1418-23.
24.Gomi Y, Sunamachi H, Mori Y, Nagaike K, Takahashi M, Yamanishi K. Comparison of the komplete DNA sequences of the Oka varicella vaccine and its parentel virus. J Virol 2002; 76(22):11447-59.
| Files | ||
| Issue | Vol 17, No 6 (2018) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijaai.v17i6.623 | |
| Keywords | ||
| ELISA Guinea pig Immunogenicity Local Seroneutralization Varicella | ||
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How to Cite
1.
Esna-Ashari F, Sabahi F, Ravanshad M, Mohammadi A. Induction of Immune Response and Protective Immunity by a Local Isolated Varicella Virus in Animal Model: A Future Candidates for Vaccine Production. Iran J Allergy Asthma Immunol. 2018;17(6):586-593.
