Immunomodulatory Effects of Human Adipose Tissue-derived Mesenchymal Stem Cells on T Cell Subsets in Patients with Rheumatoid Arthritis
-
Rasoul Baharlou
,
Nesa Rashidi
,
Abbas Ahmadi-Vasmehjani
,
Mahshid Khoubyari
,
Maryam Sheikh
,
Saiedeh Erfanian
Abstract
Adipose-derived mesenchymal stem cells (Ad-MSCs) have been reported to suppress the effector T cell responses and have beneficial effects on various immune disorders, like rheumatoid arthritis (RA). This study was designed to investigate the effects of co-cultured Ad-MSCs on peripheral blood mononuclear cells (PBMCs) of RA patients and healthy individuals, through assessing transcription factors of T cell subsets. PBMCs from RA patients and healthy donors were co-cultured with Ad-MSCs with or without Phytohaemagglutinin (PHA). The quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure the expression of T-box 21 (T-bet), GATA-binding protein-3 (GATA3), retinoid-related orphan receptor γt (ROR-γt) and forkhead box P3 (Foxp3). Based on the results, Ad-MSCs greatly upregulated Th2 and Treg cell transcription factors, i.e., GATA3 and Foxp3 (p<0.05), and downregulated Th1 and Th17 transcription factors, i.e., T-bet and RORγt (p<0.05). These results demonstrate that Ad-MSCs can result in an immunosuppressive environment through inhibition of pro-inflammatory T cells and induction of T cells with a regulatory phenotype. Therefore, they might have important clinical implications for inflammatory and autoimmune diseases such as RA.
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| Files | ||
| Issue | Vol 18, No 1 (2019) | |
| Section | Brief Communication | |
| DOI | https://doi.org/10.18502/ijaai.v18i1.637 | |
| Keywords | ||
| Adipose tissue-derived mesenchymal stem cell Foxp3 GATA3 Rheumatoid arthritis ROR-γ Regulatory T cells T-bet T helper 1 T helper 2 T helper 17 | ||
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