THE STUDY OF SOLUBLE ADHESION MOL¬ECULES (SICAM-1, SVCAM-1, SELAM-1) IN SE¬RUM AND MEMBRANE FORM OF ADHESION MOLECULES (VCAM-1, ICAM-1) ON THE MONO¬CYTES IN DIABETIC SUBJECTS TYPE 1

Abstract

The adhesion molecules are involved in adhesion of leukocytes to endothelial cells and other immune cells. Not only adhesion molecules have membrane form, but they also have soluble form. In this paper, we attempted to investigate the concen¬tration of soluble adhesion molecules in the type 1 diabetic patients (n = 40) and healthy subjects (n = 10). The results indicated that there was an increase in the concentration of soluble adhesion molecules: sICAM-1, sVCAM-1 and sELAM-1, in the sera of diabetic patients rather than the healthy ones (p = 0/00). Increased concentration of sVCAM-1 with the insulin injection dose (p = 0.01) and sIC AM-1 with duration of disease (p = 0.05) indicated significant reversel linear con-elation in the patients' groups. Also, sELAM-1 with sVCAM-1 and sICAM-1 showed significant direct linear correlation in the same group (p ~ 0.01). Adhesion mol¬ecules were determined by the sandwich ELIS A principle. One of the most important factors in the development of atherosclerosis is the ad¬hesion of monocytes to endothelial cells. Tlierefore, in this study, the percentage of expression of membrane form of VCAM-1, not ICAM-1, was increased on 10000 monocytes of diabetic patients type-1 (n = 20) in comparison with normal subjects (n = 20, p ~ 0.05). The expression tensive of membrane form of CAMs on the surface of monocytes was performed by flowcytometry technique. We concluded that the increase in sICAM-1, sVCAM-1 specially sELAM-1 and also mVCAM-1 indicated endothelial activation, stimulatability of leukocyte cells and increased interaction to endothelial cells. Although, scientists are not aware about the role of elevated CAMs concentration, it can be suggested that the in¬creasing level of sICAM-1, sVCAM-1, sELAM-1 and mVCAM may reflect cel¬lular expression, taction of CAMs in autoimmune disease and also provide a po¬tential therapeutic target for human IDDM.