Iranian Journal of Allergy, Asthma and Immunology 2017. 16(4):313-320.

Frequency of HLA DQβ1*0201 and DQβ1*0301 Alleles and Total Serum IgE in Patients with Bronchial Asthma: A Pilot Study from Pakistan
Bushra Mubarak, Nadeem Afzal, Khursheed Javaid, Rakhshanda Talib, Rabia Aslam, Waqas Latif, Saba Khaliq


In Pakistan about 3.7% of the population is suffering from asthma, a chronic inflammatory disorder of airways. Asthma has wide spectrum of predisposing factors including environment and genetics. Many studies have been performed to determine association of asthma with serum IgE and major histocompatibility complex (MHC) alleles but conflicting results were reported. Therefore, present study was designed to determine frequency of HLA-DQβ1*0201 and DQβ1*0301 alleles in patients with bronchial asthma. This case control study included 85 asthmatic patients and 85 healthy controls. HLA-DQβ1*0201 and DQβ1*0301 alleles were detected by allele specific PCR and serum IgE was determined by ELISA. Median and inter-quartile range (IQR) of total IgE level were more increased in asthma patients (585.7 IU/mL and 247.2-848.1 IU/mL) compared to healthy controls (65.1 IU/mL and 28.1-181.3 IU/mL) (p<0.001). Frequency of HLA-DQβ1*0201 and -DQβ1*0301 alleles was more in healthy controls (32% and 38%, p=0.616) as compared to bronchial asthma patients (28% and 26%, p= 0.09). There was a significant association of IgE levels and HLA-DQβ1*0201 allele. Patients positive for HLA-DQβ1*0201 allele had low level of serum IgE 357.2 IU/mL (153.9-634.3 IU/mL) compared to the patients negative for this HLA allele i.e. 642.9 IU/mL (289.8-1299.5IU/mL) (p=0.005), whereas, HLA-DQβ1*0301 allele was not associated with total serum IgE level (p=0.865). Our findings show that HLA-DQβ1*0201 and -DQβ1*0301 alleles were not associated with asthma; however, HLA-DQβ1*0201 allele was associated with low levels of total serum IgE in the study population.


Asthma; HLA-DQβ1; Immunoglobulin E; Human leukocyte antigen (HLA)

Full Text:



1.   To T, Stanojevic S, Moores G, Gershon AS, Bateman ED, Cruz AA, et al. Global asthma prevalence in adults: findings from the cross-sectional world health survey. BMC Public Health 2012; 12:204.

2.   Spencer P, Krieger B. The differentiation of chronic obstructive pulmonary disease from asthma: a review of current diagnostic and treatment recommendations. Open Nurs J 2013; 7:29-34.

3.   Dell SD, Jerrett M, Beckerman B, Brook JR, Foty RG, Gilbert NL, et al. Presence of other allergic disease modifies the effect of early childhood traffic-related air pollution exposure on asthma prevalence. Environ Int 2014; 65:83-92.

4.   Liu M, Subramanian V, Christie C, Castro M, Mohanakumar T. Immune responses to self-antigens in asthma patients: clinical and immunopathological implications. Hum Immunol 2012; 73(5):511-6.

5.   Metcalfe S, Roger M, Faucher MC, Coutlee F, Franco EL, Brassard P. The association between human leukocyte antigen (HLA)-G polymorphisms and human papillomavirus (HPV) infection in Inuit women of northern Quebec. Hum Immunol 2013; 74(12):1610-5.

6.   Mangalam AK, Taneja V, David CS. HLA class II molecules influence susceptibility versus protection in inflammatory diseases by determining the cytokine profile. J Immunol 2013; 190(2):513-8.

7.   Holgate ST. Innate and adaptive immune responses in asthma. Nat Med 2012; 18(5):673-83.

8.   Sharma S, Zhou X, Thibault DM, Himes BE, Liu A, Szefler SJ, et al. A genome-wide survey of CD4(+) lymphocyte regulatory genetic variants identifies novel asthma genes. J Allergy Clin Immunol 2014; 134(5):1153-62.

9.   Yucesoy B, Johnson VJ, Lummus ZL, Kashon ML, Rao M, Bannerman-Thompson H, et al. Genetic variants in the major histocompatibility complex class I and class II genes are associated with diisocyanate-induced Asthma. J Occup Environ Med 2014; 56(4):382-7.

10. Lama M, Chatterjee M, Chaudhuri TK. A study of the association of childhood asthma with HLA alleles in the population of Siliguri, West Bengal, India. Tissue Antigens 2014; 84(3):316-20.

11. Movahedi M, Moin M, Gharagozlou M, Aghamohammadi A, Dianat S, Moradi B, et al. Association of HLA class II alleles with childhood asthma and Total IgE levels. Iran J Allergy Asthma Immunol 2008; 7(4):215-20.

12. Choi JH, Lee KW, Kim CW, Park CS, Lee HY, Hur GY, et al. The HLA DRB1*1501-DQB1*0602-DPB1*0501 haplotype is a risk factor for toluene diisocyanate-induced occupational asthma. Int Arch Allergy Immunol 2009; 150(2):156-63.

13. Knutsen AP, Vijay HM, Kumar V, Kariuki B, Santiago LA, Graff R, et al. Mold-sensitivity in children with moderate-severe asthma is associated with HLA-DR and HLA-DQ. Allergy 2010; 65(11):1367-75.

14. Dzurilla M, Vrlik M, Homolova M, Buc M. No association between bronchial asthma and HLA-DRB1, -DQB1 alleles in the Slovak population. Bratisl Lek Listy 2013; 114(2):93-5.

15. Kontakioti E, Domvri K, Papakosta D, Daniilidis M. HLA and asthma phenotypes/endotypes: a review. Hum Immunol 2014; 75(8):930-9.

16. Olerup O, Zetterquist H. HLA-DR typing by PCR amplification with sequence-specific primers (PCR-SSP) in 2 hours: an alternative to serological DR typing in clinical practice including donor-recipient matching in cadaveric transplantation. Tissue Antigens 1992; 39(5):225-35.

17. Benjamini Y, Hochberg Y. Controlling the false discovery rate: a practical and powerful approach to multiple testing. Journal of the royal statistical society Series B (Methodological) 1995:289-300.

18. Madore AM, Vaillancourt VT, Asai Y, Alizadehfar R, Ben-Shoshan M, Michel DL, et al. HLA-DQB1*02 and DQB1*06:03P are associated with peanut allergy. Eur J Hum Genet. 2013; 21(10):1181-4.

19. Kim SH, Choi JH, Lee KW, Kim SH, Shin ES, Oh HB, et al. The human leucocyte antigen-DRB1*1302-DQB1*0609-DPB1*0201 haplotype may be a strong genetic marker for aspirin-induced urticaria. Clin Exp Allergy 2005; 35(3):339-44.

20. Mishra MN, Dudeja P, Gupta RK. Association of HLA-Class II and IgE serum levels in pediatric asthma. Iran J Immunol 2014; 11(1):21-8.

21. Gao J, Lin Y, Qiu C, Liu Y, Ma Y, Liu Y. Association between HLA-DQA1, -DQB1 gene polymorphisms and susceptibility to asthma in northern Chinese subjects. Chin Med J (Engl) 2003; 116(7):1078-82.

22. Sandeep T, Roopakala MS, Silvia CR, Chandrashekara S, Rao M. Evaluation of serum immunoglobulin E levels in bronchial asthma. Lung India 2010; 27(3):138-40.


23. An P, Barron-Casella EA, Strunk RC, Hamilton RG, Casella JF, DeBaun MR. Elevation of IgE in children with sickle cell disease is associated with doctor diagnosis of asthma and increased morbidity. J Allergy Clin Immunol 2011; 127(6):1440-6.

24. Johansson MW, Han ST, Gunderson KA, Busse WW, Jarjour NN, Mosher DF. Platelet activation, P-selectin, and eosinophil beta1-integrin activation in asthma. Am J Respir Crit Care Med 2012; 185(5):498-507.

25. Demirjian M, Rumbyrt JS, Gowda VC, Klaustermeyer WB. Serum IgE and eosinophil count in allergic rhinitis--analysis using a modified Bayes' theorem. Allergol Immunopathol (Madr) 2012; 40(5):281-7.

26. Stone KD, Prussin C, Metcalfe DD. IgE, mast cells, basophils, and eosinophils. J Allergy Clin Immunol 2010; 125(2 Suppl 2):S73-80.

27. Pien GC, Orange JS. Evaluation and clinical interpretation of hypergammaglobulinemia E: differentiating atopy from immunodeficiency. Ann Allergy Asthma Immunol 2008; 100(4):392-5.

28. Parapanissiou E, Papastavrou T, Deligiannidis A, Adam K, Kanakoudi F, Daniilidis M, et al. HLA antigens in Greek children with allergic bronchial asthma. Tissue Antigens 2005; 65(5):481-4.

29. Moffatt MF, Gut IG, Demenais F, Strachan DP, Bouzigon E, Heath S, et al. A large-scale, consortium-based genomewide association study of asthma. N Engl J Med 2010; 363(13):1211-21.


  • There are currently no refbacks.

Creative Commons Attribution-NonCommercial 3.0

This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.