Iranian Journal of Allergy, Asthma and Immunology 2014. 13(1):19-25.

Lack of association between single nucleotide polymorphism rs10818488 in TRAF1/C5 region and rheumatoid arthritis in iranian population.
Somayeh Ahmadlou, Mohsen Akhiani, Ahmad Salimzadeh, Mohammad Keramatipour


The association of rs10818488 SNP located in TRAF1/C5 region with Rheumatoid Arthritis (RA), has been picked up by genome-wide association studies. Independent studies in different populations revealed inconsistent results. The aim of this study was to investigate the possible association of this SNP with RA in Iranian population.
A total of 362 cases and 422 healthy controls were recruited in this study. Genomic DNA was extracted from whole blood and the genotyping was performed by PCR-RFLP (Polymerase Chain Reaction– estriction Fragment Length Polymorphism). A set of genotypes was confirmed by sequencing. Genotype and allele frequencies were compared between the case and control groups.
Analysis indicated a higher frequency of A allele in cases, although the difference was not statistically significant (Chi-square=2.8, p=0.09). Comparison of genotype frequencies, revealed higher frequencies of AA and AG genotypes in case group but statistically the difference was not significant (Chi-square=2.72, p=0.25).
 These findings suggest that the rs10818488 in TRAF1/C5 region is not associated with rheumatoid arthritis in Iranian population.


Association study; C5; Rheumatoid arthritis; Single nucleotide polymorphism (SNP); TRAF1

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