A Randomized Control Trial Study to Determine the Effect of Melatonin on Serum Levels of IL-1β and TNF-α in Patients with Multiple Sclerosis

  • Masoomeh Yosefifard Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
  • Gholamhassan Vaezi Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
  • Ali Akbar Malekirad Department of Biology, Payame Noor University, Tehran, Iran AND Toxicology and Diseases Group, Pharmaceutical Science Research Center, Tehran University of Medical Sciences, Tehran, Iran
  • Fardin Faraji Department of Neurology, School of Medicine, Arak University of Medical Sciences, Arak, Iran
  • Vida Hojati Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
Keywords: Interleukin 1 beta, Multiple sclerosis, Melatonin, Tumor necrosis factor-alpha


Multiple sclerosis (MS) is the most common neurological disease that happens at a young age. MS is an inflammatory disease; associated with the demyelination of the central nervous system. Therefore, some inflammatory factors are effective in the mechanism and progression of the disease. Melatonin, as a multi-effect substance including anti-inflammatory effects, can reduce symptoms of MS in patients with a change in their inflammatory factors level. In this study, 50 MS patients who were referred to the MS Society of Markazi Province were randomly selected. All patients were treated with routine MS treatment (interferon) and were divided into control (25 placebo recipients) and treatment (25 recipients of 3 mg melatonin per day for 24 weeks) groups. Anthropometric data of patients including height, weight, and age were determined. Blood samples were collected after fasting in order to determine serum levels of interleukin 1 beta (IL-1β) and tumor necrosis factor-alpha (TNF-α). Then, samples were immediately centrifuged for serum separation and sera were transferred to a freezer at -80°C and serum levels of these factors were determined; using ELISA kit. The results of this study showed that there was no significant difference between the control and treatment groups in terms of serum levels of TNF-α. However, the level of IL-1β was significantly reduced in the treatment group compared to the control group, indicating that melatonin decreases this inflammatory substance. Our findings suggest a valuable strategy in the treatment of patients who suffer from MS.


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How to Cite
Yosefifard M, Vaezi G, Malekirad AA, Faraji F, Hojati V. A Randomized Control Trial Study to Determine the Effect of Melatonin on Serum Levels of IL-1β and TNF-α in Patients with Multiple Sclerosis. Iran J Allergy Asthma Immunol. :649-654.
Original Article(s)