Association of rs1946518 C/A Polymorphism in Promoter Region of Interleukin 18 Gene and Breast Cancer Risk in Iranian Women: A Case-control Study

  • Nazila Fathi Maroufi Department of Clinical Biochemistry and Laboratory Medicine, Tabriz University of Medical Sciences, Tabriz, Iran AND Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
  • Elnaz Aghayi Department of Molecular Biology, Bonab Branch, Islamic Azad University, Bonab, Iran
  • Hamid Garshasbi Department of Genetic, Tabriz Branch, Islamic Azad University, Tabriz, Iran
  • Milad Gholampour Matin Department of Microbiology, Urmia Branch, Islamic Azad University, Urmia, Iran
  • Ahmad Babazadeh Bedoustani Biotechnology Research Center, Tabriz Branch, Islamic Azad University, Tabriz, Iran
  • Fatemeh Firouzi Amoudizaj Department of Genetic, Tabriz Branch, Islamic Azad University, Tabriz, Iran
  • Saba Hajazimian Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  • Alireza Isazadeh Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  • Sina Taefehshokr Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  • Nima Taefehshokr Division of Biosciences, Department of Life Sciences, College of Health and Life Sciences, Brunel University London, Uxbridge, Middlesex, United kingdom
  • Behzad Baradaran Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Keywords: Breast cancer, Interleukin 18, Polymorphism

Abstract

Breast cancer (BC) is the most frequently diagnosed cancer among women in the world. Genetic polymorphisms in Interleukin (IL) genes are one of the most important risk factors in BC. The aim of this study was to investigate the association of rs1946518 C/A polymorphism in the promoter region of the IL-18 gene and BC risk in Iranian women. In this case-control study, we recruited 140 women with BC as a case group and 140 age and ethnically matched women as healthy controls from East Azerbaijan, Tabriz in Iran. The genomic DNA was extracted using a salting-out method from peripheral blood leukocytes. Genotyping was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The genotype distribution in BC patients was 37.86% CC, 47.14% CA, and 15.00% AA, whereas in healthy controls these were 40.72% CC, 42.85% CA, and 16.43% AA. Statistical analysis showed that the genotype and allele frequencies of IL-18 rs1946518 C/A polymorphism were not significantly different between BC patients and healthy controls (p>0.05). The only significant difference between cases and controls was related to family history (p=0.023). In conclusion, our study indicated that IL-18 rs1946518 C/A polymorphism was not associated with BC in the Iranian women population. However, more studies on different races and geographic areas are required to determine the exact role of rs1946518 C/A polymorphism in prognosis, diagnosis, and risk of BC.

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Published
2019-11-10
How to Cite
1.
Fathi Maroufi N, Aghayi E, Garshasbi H, Gholampour Matin M, Babazadeh Bedoustani A, Firouzi Amoudizaj F, Hajazimian S, Isazadeh A, Taefehshokr S, Taefehshokr N, Baradaran B. Association of rs1946518 C/A Polymorphism in Promoter Region of Interleukin 18 Gene and Breast Cancer Risk in Iranian Women: A Case-control Study. Iran J Allergy Asthma Immunol. :671-678.
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Original Article(s)