Immunomodulatory Effects of Human Adipose Tissue-derived Mesenchymal Stem Cells on T Cell Subsets in Patients with Rheumatoid Arthritis

  • Rasoul Baharlou Cancer Research Center, Semnan University of Medical Sciences, Semnan, Iran AND Department of Immunology, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran
  • Nesa Rashidi Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  • Abbas Ahmadi-Vasmehjani Department of Immunology and Microbiology, School of Medicine, Jahrom University of Medical Sciences, Jahrom, Iran
  • Mahshid Khoubyari Department of Student Research Committee, School of Medicine, Jahrom University of Medical Sciences, Jahrom, Iran
  • Maryam Sheikh Department of Immunology, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran
  • Saiedeh Erfanian Research Center for Non-Communicable Diseases, School of Medicine, Jahrom University of Medical Sciences, Jahrom, Iran
Keywords: Adipose tissue-derived mesenchymal stem cell, Foxp3, GATA3, Rheumatoid arthritis, ROR-γ, Regulatory T cells, T-bet, T helper 1, T helper 2, T helper 17

Abstract

Adipose-derived mesenchymal stem cells (Ad-MSCs) have been reported to suppress the effector T cell responses and have beneficial effects on various immune disorders, like rheumatoid arthritis (RA). This study was designed to investigate the effects of co-cultured Ad-MSCs on peripheral blood mononuclear cells (PBMCs) of RA patients and healthy individuals, through assessing transcription factors of T cell subsets. PBMCs from RA patients and healthy donors were co-cultured with Ad-MSCs with or without Phytohaemagglutinin (PHA). The quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure the expression of T-box 21 (T-bet), GATA-binding protein-3 (GATA3), retinoid-related orphan receptor γt (ROR-γt) and forkhead box P3 (Foxp3). Based on the results, Ad-MSCs greatly upregulated Th2 and Treg cell transcription factors, i.e., GATA3 and Foxp3 (p<0.05), and downregulated Th1 and Th17 transcription factors, i.e., T-bet and RORγt (p<0.05). These results demonstrate that Ad-MSCs can result in an immunosuppressive environment through inhibition of pro-inflammatory T cells and induction of T cells with a regulatory phenotype. Therefore, they might have important clinical implications for inflammatory and autoimmune diseases such as RA.

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Published
2019-02-24
How to Cite
1.
Baharlou R, Rashidi N, Ahmadi-Vasmehjani A, Khoubyari M, Sheikh M, Erfanian S. Immunomodulatory Effects of Human Adipose Tissue-derived Mesenchymal Stem Cells on T Cell Subsets in Patients with Rheumatoid Arthritis. Iran J Allergy Asthma Immunol. 18(1):114-9.
Section
Brief Communication