Gelatinases Increase in Bleomycin-induced Systemic Sclerosis Mouse Model

  • Fateme Vafashoar Department of Immunology, Iran University of Medical Sciences, Tehran, Iran
  • Kazem Mousavizadeh Department of Molecular Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran
  • Hadi Poormoghim Scleroderma Study Group, Firoozgar Hospital, Iran University of Medical Sciences, Tehran, Iran
  • Abbas Tavasoli Department of Pathobiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  • Tahereh Musavi Shabestari Institute of Immunology and Infectious Diseases, Antimicrobial Resistance Research Center, Iran University of Medical Sciences, Tehran, Iran
  • Sayed Ali Javadmoosavi Air Pollution Research Centre, School of Public Health, Iran University of Medical Sciences, Tehran, Iran
  • Nazanin Mojtabavi Department of Immunology, Iran University of Medical Sciences, Tehran, Iran AND Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran
Keywords: Bleomycin, Gelatinase, Matrix metalloproteinase 2, Matrix metalloproteinase 9, Systemic scleroderma

Abstract

Systemic sclerosis is a fibrotic autoimmune disease in which aberrant remodeling of the extracellular matrix in organs disturbs their functionalities. The aim of this study was to investigate the expression of gelatinases on systemic sclerosis. Consequently, a mouse model of systemic sclerosis was employed and the gelatinolytic activity of gelatinases was evaluated on the fibrotic tissues of this model. Two groups of ten mice were considered in this work: a group of systemic sclerosis model and control group. For the generation of systemic sclerosis model, mice received bleomycin, while the control group was subjected to phosphate buffered saline (PBS) reception. Mice were tested for fibrosis by using trichrome staining, hydroxyproline measurement and α-SMA detection in tissue sections. Additionally, the gelatinolytic activity of matrix metalloproteinase 2 and matrix metalloproteinase 9 were measured using gelatin zymography in lungs and skin tissue homogenates. The obtained results indicated that subcutaneous injection of bleomycin-induced fibrosis in skin and lung tissues of mice. Pro and active forms of matrix methaloproteinase 9 were increased in fibrotic lung tissues (p<0.05 and p<0.01, respectively), while, the gelatinolytic activity of MMP2 was unaffected in these tissues. Additionally, in skin tissues of bleomycin-treated animals, both pro and active forms of MMP9 and MMP2 were increased (p<0.05). Pro and active forms of gelatinases increase differently in skin and lung tissues of bleomycin-induced scleroderma.

Author Biographies

Fateme Vafashoar, Department of Immunology, Iran University of Medical Sciences, Tehran, Iran

Department of Immunology Tehran Iran

Kazem Mousavizadeh, Department of Molecular Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran

Department of Molecular Medicine, Faculty of Advanced Technologies in Medicine

Hadi Poormoghim, Scleroderma Study Group, Firoozgar Hospital, Iran University of Medical Sciences, Tehran, Iran

Scleroderma Study Group, Firoozgar Hospital

Tahereh Musavi Shabestari, Institute of Immunology and Infectious Diseases, Antimicrobial Resistance Research Center, Iran University of Medical Sciences, Tehran, Iran

Institute of Immunology and Infectious Disease, Antimicrobial resistance research center

Sayed Ali Javadmoosavi, Air Pollution Research Centre, School of Public Health, Iran University of Medical Sciences, Tehran, Iran

Air Pollution Research Centre, School of Public Health

Nazanin Mojtabavi, Department of Immunology, Iran University of Medical Sciences, Tehran, Iran AND Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran

Department of immunology Iran University of medical sciences

Cellular and molecular Research center Tehran Iran

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Published
2019-04-01
How to Cite
1.
Vafashoar F, Mousavizadeh K, Poormoghim H, Tavasoli A, Musavi Shabestari T, Javadmoosavi S, Mojtabavi N. Gelatinases Increase in Bleomycin-induced Systemic Sclerosis Mouse Model. Iran J Allergy Asthma Immunol. 18(2):182-189.
Section
Original Article(s)