Investigating the Association of Orosomucoid 1-like 3 (ORMDL3) Gene Polymorphism (rs12603332) with Susceptibility to Allergic Asthma in Iranian Northwestern Azeri Population

  • Ilghar Zeinaly Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
  • Naghmeh Vossoughi Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
  • Daniel Elieh Ali Komi Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
  • Tohid Kazemi Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  • Zohreh Babaloo Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  • Alireza Razavi Department of ealth, Tehran University of Medical Sciences, Tehran, Iran
  • Mohammad Sajay-Asbaghi Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Mahnaz Sadeghi-Shabestari Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Keywords: Asthma, Orosomucoid 1-like 3, Restriction fragment length polymorphism-polymerase chain reaction, rs12603332, Single nucleotide polymorphism

Abstract

Orosomucoid 1-like 3 (ORMDL3) gene, located on chromosome 17q21, is an asthma candidate gene that encodes ORMDL3. This molecule has been reported to play a role in airway remodeling and bronchial hyper-responsiveness. In this study, we aimed to investigate the possible association of ORMDL3 single nucleotide polymorphism (SNP) (rs12603332) with susceptibility to allergic asthma in Iranian Northwestern Azeri population. 193 asthmatic patients and 185 normal individuals were included. Genomic DNA was extracted and genotyping was performed by standard restriction fragment length polymorphism-polymerase chain reaction RFLP-PCR method using BstUI restriction enzyme. Our results showed dominant presence of TC genotype and C allele in both patients (49.2% and 59.8%, respectively) and controls (48.6% and 60%, respectively). Frequency of genotypes and alleles showed no significant difference between two groups (p=0.994 and p=1.00, respectively). None of alleles could be defined as risk allele for allergic asthma (OR=0.99, 0.88-1.12, 95% CI). We failed to show significant association between ORMDL3 rs12603332 with predisposition to allergic asthma in Iranian Northwestern Azeri population. More studies with larger number of participants should be done to find more reliable results for such association.

Author Biographies

Ilghar Zeinaly, Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Naghmeh Vossoughi, Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Daniel Elieh Ali Komi, Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Tohid Kazemi, Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Zohreh Babaloo, Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Alireza Razavi, Department of ealth, Tehran University of Medical Sciences, Tehran, Iran
Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Mohammad Sajay-Asbaghi, Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Mahnaz Sadeghi-Shabestari, Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

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Published
2018-12-01
How to Cite
1.
Zeinaly I, Vossoughi N, Elieh Ali Komi D, Kazemi T, Babaloo Z, Razavi A, Sajay-Asbaghi M, Sadeghi-Shabestari M. Investigating the Association of Orosomucoid 1-like 3 (ORMDL3) Gene Polymorphism (rs12603332) with Susceptibility to Allergic Asthma in Iranian Northwestern Azeri Population. ijaai. 17(6):526-32.
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Original Article(s)