Effect of Kaempferol on Cyclooxygenase 2 (Cox2) and Cytosolic Phospholipase A2 (cPLA2) Protein Expression in BALB/c Mice

  • Da Rae Kang Department of Animal Biotechnology, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju, Republic of Korea
  • Shah Ahmed Belal Department of Animal Biotechnology, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju, Republic of Korea
  • Ho Sung Choe Department of Animal Biotechnology, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju, Republic of Korea
  • Dae Keun Shin Berry and Biofood Research Institute, Gochang, Republic of Korea
  • Kwan Seob Shim Department of Animal Biotechnology, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju, Republic of Korea
Keywords: Allergy, Cyclooxygenase, Cytosolic phospholipase A2, Inflammation, Kaempferol


Kaempferol, a phytochemical found in many edible plants, is known to alleviate diseases such as cancer, allergy, and inflammation. The objective of this study was to investigate whether kaempferol could reduce omega-6 and ovalbumin-mediated allergic reactions at lung and trachea in BALB/c mice. Mice were allocated into five groups: 1) control group (CON); 2) positive control group with orally administration of omega-6 (POS); 3) bovine serum albumin (BSA) sensitization group (with BSA injection and ovalbumin inhalation); 4) BSA+K10 group: BSA injection, 10 μg/g of kaempferol administration and ovalbumin inhalation; and 5) BSA+K20 group: BSA injection, 20 μg/g of kaempferol administration and ovalbumin inhalation. Results revealed that serum histamine level was the highest (p<0.01) in BSA group. In lung tissue and trachea, cyclooxygenase 2 (Cox2) expression was significantly (p<0.05) higher in the BSA group compared to that in other groups. However, phosphorylated cytosolic phospholipase A2 (p-cPLA2) expression in the trachea was not significantly different among groups. Taken together, results of this study suggest that kaempferol might be useful for alleviating inflammation reaction associated with Cox2 expression. However, the exact mechanism of action involved in the effect of kaempferol on inflammatory response remains unclear.


1. Grace D, Bett B, Lindahl JF, Robinson TP. Climate and livestock disease: assessing the vulnerability of agricultural systems to livestock pests under climate change scenarios. 2015.2. Kim K, Ko H, Kim H. Comparison of seasonal concentration of ammonia and hydrogen sulfide in swine house according to pig's growth stage. J Agric Life Sci 2012; 46(2)163-68.3. Sohn JH, Shin SH, Kim ST, Lee SS, Yun MJ, Cho GJ. Seroprevalence of porcine reproductive and respiratory syndrome (PRRS), porcine circovirus 2 (PCV-2), and mycoplasmal pneumonia of swine farms in Jeonbuk-Iksan. Korean J Vet Serv 2007; 30(3):305-12.4. Kantas D, Papatsiros VG, Tassis PD, Giavasis I, Bouki P, Tzika ED. A feed additive containing Bacillus toyonensis (Toyocerin®) protects against enteric pathogens in postweaning piglets. J Appl Microbiol 2015; 118(3):727-38.5. Larsen N, Thorsen L, Kpikpi EN, Stuer-Lauridsen B, Cantor MD, Nielsen B, et al. Characterization of Bacillus spp. strains for use as probiotic additives in pig feed. Appl Microbiol Biot 2014; 98(3):1105-18.6. Ghosh S, Hoselton SA, Schuh JM. μ-Chain–Deficient Mice Possess B-1 Cells and Produce IgG and IgE, but Not IgA, following Systemic Sensitization and Inhalational Challenge in a Fungal Asthma Model. J Immunol 2012; 189(3):1322-29.7. Tan JW, Israf DA, Harith HH, Hashim NFM, Ng CH, Shaari K, et al. Anti-allergic activity of 2, 4, 6-trihydroxy-3-geranylacetophenone (tHGA) via attenuation of IgE-mediated mast cell activation and inhibition of passive systemic anaphylaxis. Toxicol Appl Pharmacol 2017; 319:47-58.8. Ghosh S, Hoselton SA, Schuh JM. Characterization of CD19+ CD23+ B2 lymphocytes in the allergic airways of BALB/c mice in response to the inhalation of Aspergillus fumigatus conidia. Open Iimmunol J 2012; 5:46.9. Ghosh S, Samarasinghe AE, Hoselton SA, Dorsam GP, Schuh JM. Hyaluronan deposition and co-localization with inflammatory cells and collagen in a murine model of fungal allergic asthma. Inflamm Res 2014; 63(6):475-84.10. Jew SS, Bae ON, Chung JH. Anti-inflammatory effects of asiaticoside on inducible nitric oxide synthase and cyclooxygenase-2 in RAW 264.7 cell line. J Toxicol Pub Health 2003; 19:33-37.11. Poorani R, Bhatt AN, Dwarakanath BS, Das UN. COX-2, aspirin and metabolism of arachidonic, eicosapentaenoic and docosahexaenoic acids and their physiological and clinical significance. Eur J Pharmacol 2016; 785:116-32.12. Kang JW, Kim JH, Song K, Kim SH, Yoon JH, Kim KS. Kaempferol and quercetin, components of Ginkgo biloba extract (EGb 761), induce caspase‐3‐dependent apoptosis in oral cavity cancer cells. Phytother Res 2010; 24(S1):S77-S82.13. Devi KP, Malar DS, Nabavi SF, Sureda A, Xiao J, Nabavi SM, Daglia M. Kaempferol and inflammation: From chemistry to medicine. Pharmacol Res 2015; 99:1-10.14. Gong JH, Shin D, Han SY, Kim JL, Kang YH. Kaempferol suppresses eosionphil infiltration and airway inflammation in airway epithelial cells and in mice with allergic asthma. J Nut 2012; 142(1):47-56.15. Wang L, Tu YC, Lian TW, Hung JT, Yen JH, Wu MJ. Distinctive antioxidant and antiinflammatory effects of flavonols. J Agric Food Chem 2006; 54(26):9798-804.16. Medeiros KCP, Faustino L, Borduchi E, Nascimento RJB, Silva TMS, Gomes E, et al. Preventive and curative glycoside kaempferol treatments attenuate the TH2-driven allergic airway disease. Int Immunopharmacol 2009; 9(13-14):1540-548.17. Kempuraj D, Madhappan B, Christodoulou S, Boucher W, Cao J, Papadopoulou N, et al. Flavonols inhibit proinflammatory mediator release, intracellular calcium ion levels and protein kinase C theta phosphorylation in human mast cells. Brit J Pharmacol 2005; 145(7):934-44.18. Jin SK, Kim IS, Song YM, Hah KH. Effects of dietary oils and tocopherol supplementation on fatty acid, amino acid, TBARS, VBN and sensory characteristics of pork meat. J Anim Sci Techn 2003; 45(2):297-308.19. Simopoulos AP. The omega-6/omega-3 fatty acid ratio, genetic variation, and cardiovascular disease. Asia Pac J Clin Nutr 2008; 17(S1):131-134.20. Rule DC. Direct transesterification of total fatty acids of adipose tissue, and of freeze-dried muscle and liver with boron-trifluoride in methanol. Meat sci 1997; 46(1):23-32.21. Ghosh S, Hoselton SA, Asbach SV, Steffan BN, Wanjara SB, Dorsam GP, et al. B lymphocytes regulate airway granulocytic inflammation and cytokine production in a murine model of fungal allergic asthma. Cell Mol Immunol 2015; 12(2):202.22. Lindell DM, Berlin AA, Schaller MA, Lukacs NW. B cell antigen presentation promotes Th2 responses and immunopathology during chronic allergic lung disease. PloS one 2008; 3(9):e3129.23. Oh HA, Han NR, Kim MJ, Kim HM, Jeong HJ. Evaluation of the effect of kaempferol in a murine allergic rhinitis model. Eur J Pharmacol 2013; 718(1):48-56.24. Kim IH, Lee SH, Hong JW, Kwon OS. Optimum particle size of corn for growh performance, nutrient digestibility and feed cost in pigs. J Animal Sci Technol (Kor.) 2001; 43(2):185-192.25. Chung JY. The effects of rocuronium bromide on expressions of cyclooxygenase-2, 5-lipoxygenase, and nitric oxide synthase in bovine vascular endothelial cells. 2010; Kyung Hee University: Seoul, Korea.26. Tan X, Essengue S, Talreja J, Reese J, Stechschulte DJ, Dileepan KN. Histamine directly and synergistically with lipopolysaccharide stimulates cyclooxygenase-2 expression and prostaglandin I2 and E2 production in human coronary artery endothelial cells. J Immunol 2007; 179(11):7899-906.27. Myou S, Sano H, Fujimura M, Zhu X, Kurashima K, Kita T, et al. Blockade of eosinophil migration and airway hyperresponsiveness by cPLA2-inhibition. Nature immunol 2001; 2(2):145-149.28. Guo H, Lin W, Zhang X, Zhang X, Hu Z, Li L, et al. Kaempferol induces hepatocellular carcinoma cell death via endoplasmic reticulum stress-CHOP-autophagy signaling pathway. Oncotarget 2017; 8(47), 82207.29. Gong JH, Shin D, Han SY, Park SH, Kang MK, Kim JL, et al. Blockade of airway inflammation by kaempferol via disturbing Tyk-STAT signaling in airway epithelial cells and in asthmatic mice. Evid –vased Complement Altern Med 2013.30. Wall C, Lim R, Poljak M, Lappas M. Dietary flavonoids as therapeutics for preterm birth: luteolin and kaempferol suppress inflammation in human gestational tissues in vitro. Oxidative medicine and cellular longevity 2013.

How to Cite
Kang DR, Belal SA, Choe HS, Shin DK, Shim KS. Effect of Kaempferol on Cyclooxygenase 2 (Cox2) and Cytosolic Phospholipase A2 (cPLA2) Protein Expression in BALB/c Mice. ijaai. 17(5):428-35.
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