Imbalance of Th17/Treg in the Pathogenesis of Mice with Paraquat-induced Acute Lung Injury

  • Xia Yang Department of Emergency, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China
  • Jing-Hong Zhang The Guangxi Talent Highland for Emergency and Rescue Medicine, Guangxi Colleges and Universities Key Laboratory of Emergency Medicine Research, Nanning 530021, Guangxi, China
  • Jian-Feng Zhang Department of Emergency, The Second Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China
  • Hua Lin Department of Emergency, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China
  • Wei Chen Department of Emergency, Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning 530000, Guangxi, China
  • Li Xiang Department of Emergency, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China
  • Chao-Qian Li Department of Emergency, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China AND The Guangxi Talent Highland for Emergency and Rescue Medicine, Guangxi Colleges and Universities Key Laboratory of Emergency Medicine Research, Nanning 530021, Guangxi, China AND Department of Respiratory Medicine, Guangxi Vocational and Technical College of Health, Nanning 530023, Guangxi, China
Keywords: Acute lung injury, Paraquat, Poisoning, Th17/Treg imbalance

Abstract

Recent studies suggest that imbalances in the ratios of CD4+ T helper cell subsets, T helper-17 (Th17) and regulatory T (Treg) cells play a crucial role in the pathogenesis of acute lung injury (ALI). However, studies of the imbalance of Th17/Treg in paraquat (PQ)-induced ALI have not been reported. Therefore, we investigated whether the ratio of Th17/Treg cells in a mouse model of PQ-induced ALI contributes to pathogenesis of ALI. Male Kunming mice were randomly treated with saline (control group) or PQ (PQ-poisoned (PQP) group); mice were sacrificed at either 12 hours (PQP-12h) or 24 hours (PQP-24h and control) post-treatment. Hematoxylin-eosin and TUNEL staining procedures were performed to examine inflammation and apoptosis. The presence of Th17 and Treg cells was measured by flow cytometry; the expression of putative Th17 cytokines and transcription factors was measured by ELISA and western blot analysis. Compared with control mice, lung inflammation and apoptosis were dramatically increased in PQP mice at 12 and 24 hours after poisoning. In addition, poisoned mice displayed significant increases in the presence of CD4+IL-17+ T cells (Th17) and in the expression of IL-17A and IL-17, as measured by flow cytometry and western blot assays. This increase was most notable after 24 hours of PQ exposure. Furthermore, poisoned mice displayed marked decreases in the presence of CD4+CD25+Foxp3+ T cells (Treg) and in the expression of IL-35 and the transcription factor Foxp3. These results suggest that an imbalanced ratio of Th17/Treg cells may contribute to the pathogenesis of PQ-induced ALI.

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Published
2017-12-23
How to Cite
1.
Yang X, Zhang J-H, Zhang J-F, Lin H, Chen W, Xiang L, Li C-Q. Imbalance of Th17/Treg in the Pathogenesis of Mice with Paraquat-induced Acute Lung Injury. ijaai. 16(6):511-9.
Section
Original Article(s)