Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 17 4 2018 08 12 388 392 EN Sara Rojas-Dotor Departamento de Salud Pública, Facultad de Medicina Universidad Nacional Autónoma de México, México City, México Adriana González-Hernández Unidad de Medicina Familiar No. 7, Instituto Mexicano del Seguro Social, México City, México Francisco León-Aguilar Unidad de Medicina Familiar No. 7, Instituto Mexicano del Seguro Social, México City, México Víctor Juárez-Téllez Unidad de Medicina Familiar No. 7, Instituto Mexicano del Seguro Social, México City, México Patricia Gómez de León-Cruces Departamento de Salud Pública, Facultad de Medicina Universidad Nacional Autónoma de México, México City, México 2017 08 17 2017 12 11 Entamoeba histolytica produces, in axenic culture, the monocytes locomotion inhibitory factor (MLIF), a oligopeptide with selective anti-inflammatory properties. We evaluated the effect of MLIF on the expression of pro- and anti-inflammatory cytokines in CD4+ T lymphocytes from children with asthma and allergic rhinitis. Twelve children with severe asthma, 12 children with allergic rhinitis and 6 healthy controls were recruited for this study between May and December 2016. CD4+ T cells were cultured for 24 h at 37°C, 5% CO2 in the presence of MLIF, 1-phorbol 12-myristate 13-acetate (PMA), MLIF+PMA or RPMI. Interleukin-10 (IL-10), IL-4, interferon gamma (IFN-γ) and tumor necrosis factor alpha (TNF-α) expression levels were measured in the supernatants of T-cell cultures using the enzyme-linked immunosorbent assay (ELISA). Pro- and anti-inflammatory cytokines were inhibited by MLIF (IFN-γ p=0.0036, TNF-α p<0.001, IL-4 p=0.0082) in asthmatic patients, however IFN-γ was not significantly inhibited (NS) in patients with allergic rhinitis when compared to the RPMI group. In CD4+ T cells treated with PMA+MLIF, the expression levels of IFN-γ, TNF-α and IL-4 were strongly inhibited (p<0.001, p<0.001 and p<0.0094), compared to PMA treatment alone, for both, rhinitis and asthma. IL-10 expression was not affected by MLIF in neither of the two diseases. We conclude that MLIF alters the pro/anti-inflammatory balance and induces inhibition of IL-4, IFN-γ and TNF-α, but does not affect IL-10. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/1629 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/1629/845

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 17 4 2018 08 12 298 307 EN Afsoon Afshari Shiraz Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran AND Department of Molecular Genetics, Science and Research, Islamic Azad University, Shiraz, Iran Ramin Yaghobi Shiraz Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Negar Azarpira Shiraz Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Bita Gerami Zadeh Shiraz Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Seyed Ali Malek Hosseini Shiraz Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Mojtaba Darbouy Department of Molecular Genetics, Science and Research, Islamic Azad University, Fars, Iran Mohammad Hossein Karimi Shiraz Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran 2017 08 29 2017 12 30 Cytokines have prominent roles in activating of different T cells and shifting the immune response, in this study the role of three cytokines (IL-21, IL-23 and IL-27) is investigated in the liver transplant rejection. Three EDTA-treated blood samples were collected from each liver transplanted patient in 1st, 4th and 7th day of post-transplantation. The expression level of the mentioned cytokines was determined using real-time PCR for all samples. Also, the serum levels of cytokines were determined using ELISA tests. In acute rejection (AR) group (51 patients), mRNA expression pattern of IL-21and IL-23 showed a steady increase, but this pattern was converse for IL-27. Our results in non-acute rejection (non-AR) group (54 patients) showed an elevation in day 4 and then a decrease in day 7 for IL-21 and IL-23 genes. This pattern was converse again for IL-27 gene. In comparison between the two groups, in all 3 sampling times the mean of mRNA expression level of IL-21 and IL-23, showed an increase in AR group which this increase was significant for IL-21 in the 3rd (p=0.007) and for IL-23 in 2nd (p=0.048) and 3rd (p=0.049) sampling time, but the pattern of mRNA expression for IL-27 was contrary to the results of IL-21 and IL-23. Furthermore, ELISA technique also, showed the serum level changes the same as cytokines. In this study IL-21 and IL-23 showed pro-inflammatory properties in the liver transplant rejected patients. Also, IL-27 having different expression pattern, showed anti-inflammatory behavior which needs more considerations in future.   https://ijaai.tums.ac.ir/index.php/ijaai/article/view/1628 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/1628/849

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 17 4 2018 08 12 393 397 EN Mohammad Shahrooei Department of Microbiology and Immunology, Laboratory of Clinical Bacteriology and Mycology, KU Leuven, Leuven, Belgium AND Specialized Immunology Laboratory of Dr. Shahrooei, Sina Medical Complex, Ahvaz, Iran Abbas Fayezi Department of Allergy and Clinical Immunology, School of Medicine, Ahvaz University of Medical Sciences, Ahvaz, Iran Raheleh Shokouhi Shoormasti Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Faramarz Zakavi Specialized Immunology Laboratory of Dr. Shahrooei, Sina Medical Complex, Ahvaz, Iran AND Department of Operative Dentistry, School of Dentistry, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Leila Golpasand Hagh Specialized Immunology Laboratory of Dr. Shahrooei, Sina Medical Complex, Ahvaz, Iran AND Department of Periodontics, School of Dentistry, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Johan Van Eldere Department of Microbiology and Immunology, Laboratory of Clinical Bacteriology and Mycology, KU Leuven, Leuven, Belgium AND Department of Laboratory Medicine, University Hospitals Leuven, Leuven, Belgium Xavier Bossuyt Department of Laboratory Medicine, University Hospitals Leuven, Leuven, Belgium AND Department of Microbiology and Immunology, Experimental Laboratory Immunology, KU Leuven, Leuven, Belgium Erna Van Hoeyveld Department of Laboratory Medicine, University Hospitals Leuven, Leuven, Belgium 2017 05 19 2018 01 16 Efficient diagnosis of allergy and proper treatment need identification of the causative allergens eliciting clinical symptoms. The present study was performed to identify the most common aero- and food allergens and determine the pattern of sensitization among people of Ahvaz (southwestern Iran), one of the most polluted cities worldwide. Based on the physical examination and medical records, patients were referred to the Allergy laboratory for “in vitro” IgE determination. Specific and total IgE was determined by the ImmunoCAP system (Thermo Fisher-Phadia, Uppsala, Sweden). A total of 666 consecutive patients (51.1% female) were tested for 202 different allergens. The majority of requests (57%) belonged to food allergens. Sensitization to at least one allergen was found in 47.6% of patients. In a selected group of allergens for which specific IgE had been tested in at least 100 patients, the most common sensitizing aeroallergens were Russian thistle, grass pollen, and willow; while wheat, honey, and shrimp were the most frequent food allergens, respectively. Sensitization profiles based on measurement of specific IgE indicated that Russian thistle, grasses, and wheat were the most prevalent allergens in people with allergic symptoms living in Ahvaz. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/1471 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/1471/854

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 17 4 2018 08 12 308 317 EN Hussaini Alhassan Mohammed Department of Immunology, School of Public Health, International Campus, Tehran University of Medical Sciences, Tehran, Iran AND Department of Immunology, Faculty of Medical Laboratory Sciences, Usmanu Danfodiyo University, Sokoto, Nigeria Ali Akbar Saboor-Yaraghi Department of Immunology, School of Public Health, International Campus, Tehran University of Medical Sciences, Tehran, Iran Homayoun Vahedi Digestive Disease Research Institute, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran Ghordratollah Panahi Department of Medical Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Gholamreza Hemmasi Department of Internal Medicine and Gastroenterology, Iran University of Medical Sciences, Tehran, Iran Mir Saeed Yekaninejad Department of Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Abbas Mirshafiey Department of Immunology, School of Public Health, International Campus, Tehran University of Medical Sciences, Tehran, Iran 2017 07 13 2017 08 09 Inflammatory bowel diseases (IBD) are chronic relapsing immune-mediated disorders that result from an aberrant immunological response. IBD comprises of Crohn's disease (CD) and ulcerative colitis (UC). The precise aetiology of IBD has not been fully understood, however, recent studies support the hypothesis that patients with IBD have a dysregulated immune response to endogenous bacteria in the gastrointestinal tract (GIT). The increasing number of hospitalisation coupled with the high economic burden faced by IBD patients, calls for more concerted research efforts, to design a potent and credible treatment option for these strata of patients. This research was designed to test the efficacy and potency of β-D Mannuronic acid (M2000) in the treatment of IBD. Ten ml of blood was aseptically collected from 24 IBD patients and 24 normal controls. PBMC was isolated and stimulated with 1 µg/mL of LPS and incubated for 4 hours. The cells were later treated with 10 µg/mL or 50 µg/mL of Mannuronic acid and incubated for 24 hours at 370C under 5% CO2 and 100% humidity. After the incubation, RNA was extracted from the cells, cDNA was synthesised, and the expression of the gene was evaluated using quantitative real-time PCR. The result indicated a significant down-regulation of RORC and IL-17 genes expression, while the expression of IL-4 and GATA3 genes were significantly up-regulated. These research findings have shown that M2000 a biocompatible agent, that has an immunotherapeutic, immunomodulatory and immunosuppressive effects on the PBMC of IBD patients.  https://ijaai.tums.ac.ir/index.php/ijaai/article/view/1564 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/1564/850

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 17 4 2018 08 12 318 325 EN Yugang Xu Department of General Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China AND Department of General Surgery, Tai'an Central Hospital, Tai'an, Shandong, China Ming Cheng Department of General Surgery, Tai'an Central Hospital, Tai'an, Shandong, China Lei Mi Department of General Surgery, Tai'an Central Hospital, Tai'an, Shandong, China Yunping Qiu Department of General Surgery, Tai'an Central Hospital, Tai'an, Shandong, China Wenli Hao Department of General Surgery, Tai'an Central Hospital, Tai'an, Shandong, China Leping Li Department of General Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China 2017 08 11 2017 12 05 Mir-22-3p is associated with many important biological processes, including neuroprotection, tumorigenesis, and various other tumor progressions. Our study aimed to investigate the roles of Mir-22-3p in chemosensitivity of gastrointestinal stromal tumor (GIST-T1) cells to cisplatin and explore its underlying mechanisms. Mir-22-3p high-expressing cell line was established by transfecting GIST-T1 cell line cells with Mir-22-3p mimic. After treatment with cisplatin (10 μM), Cell counting kits-8 (CCK-8) method was used to detect the cell viability. Flow cytometry was applied to measure the degree of cell apoptosis. Scratch wound healing test was used to detect the migration ability of cells. The protein and mRNA levels of the phosphatase and tensin homolog deleted on chromosome ten (PTEN)/phosphatidylinositol 3 kinase (PI3K)/protein kinase B (Akt) pathway-related factors were analyzed by Western blot and qRT-PCR. The mRNA level of Mir-22-3p was increased in transfected GIST-T1 cells compared with that in control cells. The survival rate and Bcl-2/Bax ratio of GIST-T1 cells treated with both Mir-22-3p analogue and cisplatin were significantly decreased, while the apoptosis rate and protein level of caspase-3 were significantly increased (p<0.05). In addition, the mRNA and protein levels of PTENwere significantly increased in cells treated with both Mir-22-3p analogue and cisplatin (p<0.05), while the expression levels of PI3K and Akt were significantly decreased (p<0.05). Mir-22-3p overexpression can increase the chemosensitivity of cisplatin in human gastrointestinal stromal tumor cells by PTEN/PI3K/Akt pathway.   https://ijaai.tums.ac.ir/index.php/ijaai/article/view/1622 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/1622/851

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 17 4 2018 08 12 326 335 EN Sorour Shojaeian Department of Biochemistry, Medical Genetics, Nutrition, Alborz University of Medical Sciences, Karaj, Iran Abdolamir Allameh Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Mahmood Jeddi-Tehrani Department of Hybridoma, Monoclonal Antibody Research Center, Avicenna Research Institute, Academic Center for Education, Culture and Research (ACECR), Tehran, Iran Roya Ghods Oncopathology Research Center, Iran University of Medical Sciences, Tehran, Iran AND Department of Molecular Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medicine Sciences, Tehran, Iran Jaleh Shojaeian University of Maryland School of Pharmacy, Baltimore, Maryland, USA Akram-Sadat Tabatabaei-Panah Department of Biology, Faculty of Basic Sciences, Islamic Azad University, East Tehran Branch (Ghiamdasht), Tehran, Iran Amir-Hassan Zarnani Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran AND Reproductive Immunology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran AND Immunology Research Center (IRC), Iran University of Medical Sciences, Tehran, Iran 2017 08 01 2018 02 13 In recent years, a lot of attention has been paid to quantum dot (QD) nanoparticles as fluorescent sensors for sensitive and accurate detection of cancer biomarkers. Here, using a homemade specific monoclonal antibody against CA125 and QD525- or FITC-labeled probes, expression of this marker in an ovarian cancer cell line and cancer tissues were traced and optical properties of fluorophores were compared qualitatively and quantitatively. Our results clearly showed that besides lower background and exceptionally higher photobleaching resistance, QD525 exhibited higher fluorescent intensity for both ovarian cancer cell and tissues at different exposure times (p<0.0001) and excitation filter sets (p<0.0001) exemplified by significantly higher staining index (p<0.016). More importantly, the FITC-labeled probe detected antigen-antibody complex at minimum concentration of 0.3 mg/mL of anti-CA125, while reactivity limit decreased to 0.078 mg/mL of anti-CA125 when QD525-labeled probe was applied showing four times higher reactivity level of QD525 probe compared to the same probe labeled with FITC. Based on our results, it seems that QDs are inimitable tags for sensitive detection and localization of ovarian cancer micrometastasis and molecular demarcation of cancer tissues in surgical practice, which subsequently figure out accurate therapeutic approaches.  https://ijaai.tums.ac.ir/index.php/ijaai/article/view/1603 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/1603/840

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 17 4 2018 08 12 336 345 EN Dariyush Raeisi Department of Internal Medicine, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran Mohammad Erfan zare Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran Atefeh Nasir Kansestani Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran AND Nosocomial Infection Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran Hamidraza Sherkatolabbasieh Department of Pediatric, Lorestan University of Medical Sciences, khorramabad, Iran Shiva Shafeizadeh Department of Internal Medicine, Lorestan University of Medical Sciences, khorramabad, Iran 2017 04 26 2017 10 02 Pathogenesis of systemic lupus erythematosus (SLE) is complex and multi-factorial. Among various suggested mechanisms for the disease, the hormonal theory has been considered as one of the most important mechanisms. Recently, the association of sex hormones with manifestations of antiphospholipid antibody syndrome (APLS) has been hypothesized. The aim of present study was to assess the serum levels of anticardiolipin antibody (ACA), sex hormones and prolactin in SLE female patients and their association with the disease. This study comprised 40 SLE female patients and 41 healthy age-matched female subjects. For all patients and controls, the serum levels of ACA (IgG and IgM), estradiol, testosterone, progesterone, dehydroepiandrosterone sulfate (DHEA-S) and prolactin were measured by ELISA method. Our study revealed that serum levels of testosterone, DHEA-S and progesterone were significantly lower in SLE patients than control (p<0.001). However, serum levels of estradiol and prolactin were significantly higher in SLE patients compared to controls (p<0.001). There was a significant difference between mild and moderate severity patients group for ACA positivity (95% CI 13.67-41.3; p=0.03). Also, SLE patients with positive ACA showed significantly lower (p<0.001) serum levels of testosterone, DHEA-S and progesterone and significantly higher (p<0.001) estradiol and prolactin serum levels compared to negative ACA patients. The results of our study indicated that expression and metabolism of sex hormones and prolactin are different in female SLE patients compared to healthy subjects. It seems, change in serum levels of these hormones is related to higher SLE disease activity, increased thrombotic risks and increased renal involvement.  https://ijaai.tums.ac.ir/index.php/ijaai/article/view/1435 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/1435/852

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 17 4 2018 08 12 346 360 EN Bahare Laribi Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Mohammad Ali Sahraian MS Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran Mehdi Shekarabi Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Rahimeh Emamnejad Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran Mohsen Marzban Department of Neurosciences, Tehran University of Medical Sciences, Tehran, Iran Shokufeh Sadaghiani MS Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran Maryam Izad Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran AND MS Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran 2017 08 01 2017 08 27 Fingolimod is a novel immunomodulatory drug used in patients with relapsing multiple sclerosis (MS) which reversibly inhibits egress of lymphocytes from lymph nodes. In this longitudinal study, the frequency of Interferon- gamma (IFN-γ)+, IL4+, IL17+ and IL10+ CD4+ and CD8+ T cell subsets were measured in Fingolimod treated patients before and after 12 months’(12M) therapy using flow cytometry and compared to those of naive, Betaferon treated MS patients and healthy individuals. Additionally, the level of transcription factor IRF4 and IL-6, IL-23, TGF-β1 cytokines, required for differentiation of IL-17+ T cells, were assessed by RT-PCR and ELISA, respectively. In Fingolimod treated MS patients, we observed a significant decrease in the percentage of IFN-γ+/IL17+ CD4+ and CD8+ T cell subsets. In contrast, Fingolimod increased IL10+ CD4+ T cells. We also showed that IFN-γ+IL17+ co-producing CD8+ T cells were reduced in patients under fingolimod therapy. furthermore, Fingolimod could reduce the expression level of IRF4 in patients while IL6 was increased in the supernatant of cultured peripheral blood mononuclear cells. Our data showed that Fingolimod treatment alters CD4+ and CD8+ T cell subsets and reduces expression of IRF-4, which affects the proportion of pathogenic memory T cells in peripheral blood. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/1601 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/1601/842

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 17 4 2018 08 12 361 371 EN Mahdi Zavvar Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Mohsen Abdolmaleki Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Hamid Farajifard Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Farshid Noorbakhsh Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran kayhan Azadmanesh Department of Virology, Pasteur Institute of Iran, Tehran, Iran Mohammad Vojgani Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Mohammad Hossein Nikcnam Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran AND Molecular Immunology Research Center, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran 2017 08 05 2017 12 18 Regulatory T cells (Tregs) play a major role in the prevention of autoimmune diseases. Transfer of Foxp3 gene into conventional T cells converts their phenotype to regulatory T cells. Therefore, the question arises as to whether adoptively transferred in vitro differentiated Treg cells specific for a locally expressed antigen might have better inhibitory effects on the progression of the disease as compared with antigen-nonspecific T reg cells. Herein, we investigated the therapeutic potential of primed and unprimed retrovirus mediated Foxp3-overexpression T cells following intravenously injected of these cells into affected rats with collagen-induced arthritis (CIA), an animal model of rheumatoid arthritis. Our analyses demonstrate that systemic administration of collagen II primed Foxp3-transduced T cells could markedly ameliorate CIA inflammatory responses at clinical (p<0.0014) and pathological exchanges including cellular infiltration (p=0.002), bone erosion (p=0.0013) and synovial hyperplasia (p=0.002). In contrast, collagen II unprimed Foxp3-transduced T cells like as collagen II primed or unprimed GFP-transduced T cells did not reveal any beneficial effects on arthritis features as compared with untreated group (p>0.05). Therefore, we believe that collagen II primed Foxp3-transduced T cells are interacting locally and systemically with immune cells which reveled with decreasing of T cells infiltration into joints along with specific CII IgG production. Considering the results described here, it appears that the using patients' T cells which previously exposed to specific antigens may have more effective therapeutic advantage in the production of induced regulatory T cells in the treatment of arthritis. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/1609 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/1609/853

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 17 4 2018 08 12 372 378 EN Laya Takbiri Osgoei Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran Kazem Parivar Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran Marzieh Ebrahimi Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, Tehran, Iran Esmaeil Mortaz Clinical Tuberculosis and Epidemiology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran AND Department of Immunology, Faculty of Medicine, Shahid Beheshti University of  Medical Sciences, Tehran, Iran 2017 06 13 2017 09 19 The underlying mechanisms of how nicotine affects cord umbilical cells remain largely elusive. Nicotine rapidly crosses the blood-brain barrier (10 to 20 s) and binds to nicotinic acetylcholine receptors (nAChRs). Nicotine considered as a major compound found in cigarette smoke and the mechanism of nicotine action in immune response is not well understood. Cigarette smoke well known by activation of toll like receptors (TLRs) especially TLR4 and 9 which stimulates the immune response by induction of releases of cytokines mainly CXCL-8 which in turn triggers lungs reactions specially induction of neutrophils recruitments. In this study we isolated human umbilical mononuclear cells (UCBMC) from umbilical cord blood and exposed to the nicotine for detection any cytokines and TLRs modulation. We have found that nicotine (at concentration 0.01µM) induced release of TNF-a and IL-6 but not CXCL-8 production. Besides we have shown that nicotine did not effect on TLR4 surface expression however up-regulated the TLR2 surface expression. Moreover expression of CD11a and CXCR4 after nicotine incubation was upregulated as demonstrated by flow cytometry analysis, These data indicated that nicotine by stimulation of inflammatory cytokines induces immune response. The present study provides evidence that nicotine selectively regulates the release of cytokines and expression of TLRs. Further studies are needed to exploring details of its effects and signaling. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/1509 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/1509/839

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 17 4 2018 08 12 379 387 EN Marzieh Eghtedardoost Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Zuhair Hassan Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Tooba Ghazanfari Immunoregulation Research Center, Shahed University, Tehran, Iran Alireza Sadeghipour Pathology Department, Rasoul Akram Medical Complex, Iran University of Medical Sciences, Tehran, Iran Mostafa Ghanei Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran AND Department of Respiratory, Pasteur Institute of Iran, Tehran, Iran 2017 07 22 2017 09 13 Despite many years having passed since exposure to sulfur mustard (SM) gas, there are many exposed subjects who are still suffering from delayed pulmonary complications. The levels of pro-inflammatory cytokines in the lung of these subjects have not been investigated in delay phase. In this study, we evaluated mRNA expression of pro-inflammatory  cytokines (tumor necrosis factor alpha (TNFα), tumor necrosis factor receptor type 1 (TNFR1), and interleukin 1 beta (IL-1β)  ) in lung biopsy of SM-exposed subjects and compared them with control (non-exposed) subjects. We used formalin-fixed, paraffin-embedded (FFPE) tissue for this purpose. Lung FFPE blocks of SM-exposed subjects (30 samples) and a control group (30 samples) were collected from archival pathology department. The total mRNA of FFPE tissues were extracted and the mRNA expression of pro-inflammatory  cytokines were determined by quantitative Real Time PCR (RT-qPCR). The obtained results from two groups were compared to each other and non-parametric statistical analyses were carried out on them. Our studies showed that the mRNA expression of TNFα, TNFR1 and IL-1β  in lung tissue of SM injured and control people have no significant difference (p-value= 0.159, 0.832 and 0.314 respectivly). TNFR1 showed direct correlation with TNFα (r=0.867, p=0.002) and IL-1β (r= 0.65, p=0.006). The evaluation of mRNA expression in pro-inflammatory cytokines in lung of SM-exposed subjects after 20 years showed that these mediators are similar to those of non-exposed group and there was no acute inflammation in lung of these patients. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/1582 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/1582/841