Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 11 4 2012 12 15 276 281 EN Ebrahim Nadi Division of Pulmonary Sciences and Critical Care Medicine, Beheshti Hospital, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. Mehrdad Hajilooi Department of Immunology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. Davood Babakhani Division of Pulmonary Sciences and Critical Care Medicine, Beheshti Hospital, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. Alireza Rafiei Department of Immunology, Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Mazandaran, Iran. 2015 10 16 Asthma is considered as a chronic inflammatory airway disease and defined as increased tracheobronchial responsiveness to variety of stimuli. Edema and inflammatory cell infiltration in airway is observed in the asthmatic patients. One of the essential changes in inflammation is adhesion of leukocyte to endothelium and transmigration of leukocytes to the sites of inflammation. Unfortunately, little is known about the role of platelet endothelial cell adhesion molecule-1 (PECAM-1) polymorphism in asthma inflammatory process. The purpose of this study was to determine whether PECAM-1 polymorphisms affect the risk of asthma or not.Forty-five asthmatic patients (including 27 men and 18 women) and 45 healthy volunteers (11 men and 34 women) were studied. To determine the severity of the asthmas situation, a questionnaire was prepared asking the following information: age, sex, clinical signs and symptoms and past medical history. All subjects were genotyped for PECAM-1 polymorphism by using amplification refractory mutation system -polymerase chain reaction (ARMS-PCR). The genotype distribution of PECAM-1 80 Val/Met polymorphism in all asthmatic patients were Val/Val while non asthmatic controls were 95.6% Val/Val and 4.4% Val/Met. However, these differences were not statistically significant (p<0.05). The allele and genotype frequencies of PECAM-1 125 Val/Leu polymorphism were significantly different between asthmatic patients and controls. On the other hand, the presence of 125 Leu allele was associated with an increasing risk of asthma with an odds ratio of 2.8 (95% CI; 1.5-5.3, p=0.002). Our findings suggest that the PECAM-1 125 Val/leu polymorphism might be a genetic factor that may be associated with asthma. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/551 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/551/496

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 11 4 2012 12 15 282 293 EN Dejan Trajkov Institute of Immunobiology and Human Genetics, Faculty of Medicine, University Ss. Cyril and Methodius, Skopje, Republic of Macedonia. Aleksandar Petlichkovski Institute of Immunobiology and Human Genetics, Faculty of Medicine, University Ss. Cyril and Methodius, Skopje, Republic of Macedonia. Olivija Efinska-Mladenovska Institute of Immunobiology and Human Genetics, Faculty of Medicine, University Ss. Cyril and Methodius, Skopje, Republic of Macedonia. Slavica Hristomanova Institute of Immunobiology and Human Genetics, Faculty of Medicine, University Ss. Cyril and Methodius, Skopje, Republic of Macedonia. Eli Djulejic Institute of Immunobiology and Human Genetics, Faculty of Medicine, University Ss. Cyril and Methodius, Skopje, Republic of Macedonia. Meri Kirijas Institute of Immunobiology and Human Genetics, Faculty of Medicine, University Ss. Cyril and Methodius, Skopje, Republic of Macedonia. Aleksandar Senev Institute of Immunobiology and Human Genetics, Faculty of Medicine, University Ss. Cyril and Methodius, Skopje, Republic of Macedonia. Mirko Spiroski Institute of Immunobiology and Human Genetics, Faculty of Medicine, University Ss. Cyril and Methodius, Skopje, Republic of Macedonia. 2015 10 16 The aim of this study was to analyze 22 cytokine polymorphisms in the Roma population from the Republic of Macedonia. The Roma population consists of 77 healthy unrelated individuals, residents of different geographical regions of the Republic of Macedonia (Skopje, Gostivar, and Kochani). Blood samples were collected after obtaining written consent. DNA was isolated from peripheral blood and 22 polymorphisms were typed: IL1A -889, IL1B -511, IL1B +3962, IL1R pst1 1970, IL1RN mspa11100, IL4RA +1902, IL12 -1188, IFNG utr5644, TGF-β1 cdn10, TGF-β1 cdn25, TNF-α -308, TNF-α -238, IL-2 -330, IL-2 +166, IL-4 -1098, IL-4 -590, IL-4 -33, IL-6 -174, IL-6 565, IL-10 -1082, IL-10 -819, and IL-10 -592. Cytokine genotyping was performed by PCR-SSP. The population genetics analysis package, PyPop, was used for analysis of the cytokine data. Fnd was negative and significantly different from 0 for IL-4 -590 (p of F=0.006), IL-10 -1082 (p of F=0.010), IFN utr5644 (p of F=0.024), IL-4 -1098 (p of F=0.026) and TGF-1 cdn25 (p of F=0.001) alleles, as well as for IL-2 haplotypes (p=0.025). Several SNPs (IL-12B -1188, IL-2 -330, IL-4 -1098, IL-4 -590, and IL-10 -1082) were not in HWP (p<0.05). A few SNPs (IL-12B -1188, IL-2 -330, IL-4 -1098, IL-4 -590, and IL-10 -1082) and several observed frequencies of cytokine diplotypes (IL-2/GG:TG, IL-2/TG:TG, IL-4/GCC:GCC, IL-4/TTC:TTC, IL-4/TTT:TTC, IL-10/GCC:GCC, IL-10/ATA:GCC, IL-10/ACC:GCC, and IL-10/ACC:ATA) were not in HWP and were significantly different from the expectations. Hardy Weinberg proportion could not be calculated for TNF genotypes and diplotypes because nearly all genotypes and diplotypes belong to GG genotype or GG:GG diplotype. The results of cytokine polymorphisms in Roma population can be used for characterization of the current genetic profile of the Gypsies, anthropological comparisons, as well as for the association studies with different diseases. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/550 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/550/498

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 11 4 2012 12 15 294 300 EN Mahdi Mahmoudi Molecular Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran AND Rheumatology Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran AND Department of Immunology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran Ahmad Reza Jamshidi Rheumatology Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran Ali Akbar Amirzargar Molecular Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran AND Department of Immunology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran Elham Farhadi Molecular Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran Keramat Nourijelyani Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Sasan Fallahi Rheumatology Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran AND Department of Rheumatology, Shafa Hospital, Kerman University of Medical Sciences, Kerman, Iran Mona Oraei Department of Immunology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran Sahar Noori Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Mohammad Hossein Nicknam Molecular Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran AND Department of Immunology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran 2015 10 16 Ankylosing Spondylitis (AS) is an inflammatory arthritis, which affects mainly spine and sacroiliac joints. According to recent studies, ERAP1 is the second most common candidate gene for  AS susceptibility after HLA-B27. The  aim of this study was to  determine the association of ERAP1 gene polymorphisms with AS in Iranian population. The study group comprised 387 Iranian AS patients and 316 healthy controls from Iran. Using Real Time PCR allelic discrimination method,  we genotyped four SNPs (rs30187, rs469876, rs13167972 and rs27434) of ERAP1. We found  that  rs30187 and rs27434 were significantly associated with AS in Iranian population (P=6×10-5, P=7×10-3,  respectively). The rs30187 T/T genotype was associated with AS compared  with C/C  genotype (P=1.5×10-5).  The  rs27434 G/G genotype was inversely associated with  AS (P=5×10-3).  Two  specific haplotypes  including:  rs30187/ rs469876/ rs13167972/ rs27434 TAAA and CAGG  were associated with increased and decreased risk of AS in Iranian population, respectively. These results indicated that ERAP1 SNPs and haplotypes were associated with AS in Iranian population. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/549 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/549/499

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 11 4 2012 12 15 301 307 EN Aylin Ozgen Alpaydin Department of Pulmonary Diseases, Dokuz Eylul University Medical Faculty, İzmir, Turkey. Mine Bora Department of Pulmonary Diseases, Celal Bayar University Medical Faculty, Manisa, Turkey Arzu Yorgancioglu Department of Pulmonary Diseases, Celal Bayar University Medical Faculty, Manisa, Turkey Aysin Sakar Coskun Department of Pulmonary Diseases, Celal Bayar University Medical Faculty, Manisa, Turkey Pinar Celik Department of Pulmonary Diseases, Celal Bayar University Medical Faculty, Manisa, Turkey 2015 10 16 Asthma control and quality of life are expected to be correlated. We aimed to evaluate the association of asthma control test (ACT) with asthma quality of life questionnaire (AQLQ) and guideline based control assessment. We also aimed to investigate the impact of therapy adjustment according to ACT score on AQLQ A total of 101 asthmatic patients were included. ACT, AQLQ and Global Initiative for Asthma (GINA) based control assessments were performed. Based on ACT, treatment was adjusted by stepping down in controlled and stepping up in uncontrolled/partly controlled patients. In some controlled/partly controlled patients, no therapy adjustment was done. After 3-months the same parameters were reevaluated.We found a statistically significant association between ACT and AQLQ, a one point increase in ACT was associated with a 0.129 point increase in AQLQ. ACT scores increased significantly in the step-up group; however AQLQ total scores were not affected after therapy adjustment. We found that ACT was concordant with GINA recommended control classification in the first (kappa=0.511, 7.718) and third months (kappa=0.599, 7.912) (P<0.001 for both).We determined an association between ACT and AQLQ. ACT was also found fairly concordant with GINA. However, treatment adjustment according to ACT was not found satisfactory in terms of quality of life. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/548 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/548/501

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 11 4 2012 12 15 308 315 EN Abdollah Jafarzadeh Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran AND Department of Immunology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. Fazel Shokri Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran AND Monoclonal Antibody Research Center, Avicenna Research Institute (ACECR), Tehran, Iran. 2015 10 16 The immune response to hepatitis B surface antigen (HBsAg) is influenced by several factors, of which HLA antigens and balanced secretion of Th1/Th2 cytokines play important roles. The aim of this study was to evaluate the influence of HLA antigens on cytokine secretion by HBsAg-stimulated peripheral blood mononuclear cells (PBMC) from healthy neonates vaccinated with recombinant HBsAg. PBMCs were isolated from 48 Iranian neonates vaccinated with a recombinant HBV vaccine. The cells were stimulated in vitro with rHBsAg and the concentration of IL-4, IL-10, IL-12 and IFN-γ were quantitated in culture supernatant by sandwich ELISA. HLA typing was performed by microlymphocytotoxicity method. Significant diminished secretion of both Th1 (IFN-γ) and Th2 (IL-4, IL-10) cytokines was observed in HBsAg-stimulated PBMC from vaccinees expressing the HLA-DR7 compared to DR7 negative vaccinees. Similarly, lower production of these cytokines was also observed in vaccinees with DR7-DR53-DQ2, B7-DR7-DR53-DQ2 and A2-DR7-DR53-DQ2 haplotypes (p<0.05, p <0.005). While HBsAg-stimulated PBMC of DR13+ subjects produced lower levels of Th2-type cytokines (IL-4 and IL-10), those of HLA-B8+ or HLA-A9+ subjects produced higher levels of Th2-type cytokines. Cytokine secretion in response to PHA mitogen was not associated with a given HLA antigen or haplotype and was similarly represented in all groups of subjects irrespective of their HLA complex. These results indicate that HLA antigens may differentially influence cytokine secretion by HBsAg-specific T-cells of healthy neonates vaccinated with recombinant HB vaccine. This phenomenon may have an important implication for control of the immune response to HBsAg vaccine. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/547 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/547/502

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 11 4 2012 12 15 316 323 EN Arzu Babayigit Hocaoglu Department of Pediatrics, Dokuz Eylul University Hospital, Division of Allergy, Balcova, Izmir, Turkey Ozkan Karaman Department of Pediatrics, Dokuz Eylul University Hospital, Division of Allergy, Balcova, Izmir, Turkey Duygu Olmez Erge Department of Pediatrics, Dokuz Eylul University Hospital, Division of Allergy, Balcova, Izmir, Turkey Guven Erbil Department of Histology and Embriology, Dokuz Eylul University Hospital, Balcova, Izmir, Turkey Osman Yilmaz Department of Multidisciplinary Animal Laboratory Balcova, Dokuz Eylul University Hospital, Izmir, Turkey Bijen Kivcak Department of Pharmacognosy, Ege University, Faculty of Pharmacy, Bornova, Izmir, Turkey H Alper Bagriyanik Department of Histology and Embriology, Dokuz Eylul University Hospital, Balcova, Izmir, Turkey Nevin Uzuner Department of Pediatrics, Dokuz Eylul University Hospital, Division of Allergy, Balcova, Izmir, Turkey 2015 10 16 Hedera helix  is widely used to treat bronchial asthma for many years. However, effects of this herb on lung histopathology is still far from clear. We aimed to determine the effect of oral administration of Hedera helix on lung histopathology in a murine model of chronic asthma. BALB/c  mice  were  divided  into  four  groups;   I  (Placebo),  II  (Hedera  helix), III (Dexamethasone) and IV (Control). All mice except controls were sensitized and challenged with ovalbumin. Then, mice in group I received saline, group II 100 mg/kg Hedera helix and group III 1 mg/kg  dexamethasone via orogastic gavage once daily for one week. Airway histopathology was evaluated by using light and electron microscopy in all groups. Goblet  cell numbers and thicknesses of basement membrane were found  significantly lower in group II, but there was no statistically significant difference in terms of number of mast cells, thicknesses of epithelium and subepithelial smooth muscle layers between group I and II. When Hedera helix and dexamethasone groups were compared with each other, thickness of epithelium, subepithelial muscle layers, number of mast cells and goblet cells of group III were significantly ameliorated when compared with the group II. Although Hedera helix administration reduced only goblet cell counts and the thicknesses of basement membrane  in the  asthmatic airways, dexamethasone ameliorated all histopathologic parameters except thickness of  basement  membrane  better  than  Hedera helix. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/546 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/546/504

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 11 4 2012 12 15 324 328 EN Mansoureh Shariat Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran. Zahra Pourpak Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Mojtaba Khalesi Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Anoshirvan Kazemnejad Department of Biostatistics, Tarbiat Modares University, Tehran, Iran Laleh Sharifi Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Golnoosh Souzanchi Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Masoud Movahedi Department of Immunology and Allergy, Children Medical Center, Tehran University of Medical Sciences, Tehran, Iran Mohammad Gharagozlou Department of Immunology and Allergy, Children Medical Center, Tehran University of Medical Sciences, Tehran, Iran Maryam Mahlooji Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Mostafa Moin Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran 2015 10 16 Allergic Rhinitis (AR) is a common global health problem with approximately one quarter of  the  world population  affected. The  Quality of  Life (QOL)  of  sufferers with AR is significantly affected. The aim of this study was to evaluate the QOL of adults with AR. This study was designed for adults with AR above 18 years old. The study was conducted using a valid Rhinitis Quality of Life Questionnaires (RQLQ) which was completed for each patient during clinic visit and analyzed by applying statistical methods. One-hundred  and ten AR patients participated in this study. Mean age of these patients was 32 years old and 62% were female. The   correlation  between  severity  of   AR  and  QOL   impairment  was  significant. Frequencies of mild persistent, moderate-severe persistent, mild intermittent and moderate- severe intermittent types of AR were 18%, 34.5%, 9% and 38%, respectively. Completed RQLQ indicated that about 55% of the cases were suffering from severe disturbances in their  QOL.   Furthermore,   congestion  (88%)  was  the  most  common   symptom.  The correlation between congestion and QOL reduction was significant. The correlation between congestion and sleep impairment was significant. AR was more common in young as well as female patients and their QOL  was affected more than the others. The results showed a good relevancy between severity of symptoms and QOL scores. Consisting with ARIA classification, it was found that reduction in the quality of life is higher in patients with moderate-severe intermittent and persistent asthma. Nasal congestion was a bothersome and prevalent symptom in AR that was responsible for sleep problems. Therefore, nasal congestion was associated with sleep-disordered breathing, nocturnal sleep impairment, day time fatigue and somnolence which finally lead to QOL impairment. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/545 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/545/505

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 11 4 2012 12 15 329 335 EN Violeta Dancheva Sector of Disaster Medicine, Medical University, Pleven, Bulgaria. Lyudmil Terziev Sector of Clinical Immunology and Allergology, Medical University, Pleven, Bulgaria Veneta Shopova Sector of Disaster Medicine, Medical University, Pleven, Bulgaria. Galya Stavreva Sector of Clinical and Experimental Pharmacology,  Medical University, Pleven, Bulgaria 2015 10 16 We aimed to study the MnTnHex-2-PyP effect on some markers of lung antioxidant defence system in mice asthma model.The study was carried out on 28 C57B1/6 mice divided into four treatment groups: group 1 - controls; group 2 - injected and inhaled with ovalbumin; group 3 - treated with MnTnHex-2-PyP and inhaled with phosphate buffered saline; group 4 - injected with ovalbumin and MnTnHex-2-PyP but also inhaled with ovalbumin. On days 24, 25 and 26, mice from groups 1 and 2 were inhaled with PBS for 30 min, and those from groups 2 and 4 were given a 1% ovalbumin solution. One hour before inhalation, and 12 hours later the animals from groups 1 and 2 were injected i.p. with 100 μl PBS, and those from groups 3 and 4 received a 100 μl MnTnHex-2-PyP solution in PBS, сontaining 0,05mg/kg. The animals were killed by exsanguination 48 hours after the last inhalation for obtaining a lung homogenate. The activities of superoxide dismutase, catalase, glutathione peroxidase and the non-protein sulphhydryl group content in the lung homogenate were investigated. Ovalbumin decreased the activities of superoxide dismutase (p=0.01), catalase (p=0.002), glutathione peroxidase and non-protein sulphhydryl groups content (p<0.001) in comparison to controls. In group 4 (ovalbumin and MnTnHex-2-PyP) the activities of superoxide dismutase (p=0.044), catalase (p=0.045), glutathione peroxidase (p=0.002), and the non-protein sulphhydryl groups content (p<0.001) were significantly increased compared to ovalbumin (group 2).MnTnHex-2-PyP restored the activities of basic enzymes in the lung antioxidant defence system in ovalbumin-induced asthma mice model, 48 hours after the last nebulization. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/544 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/544/506

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 11 4 2012 12 15 336 339 EN Fatemeh Behmanesh Allergy Research Center, Ghaem Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Farah Ashrafzadeh Department of Pediatric Neurology, Faculty of Medicine, University of Medical Sciences, Mashhad, Iran Abdoreza Varasteh Department of Immunology and Allergy, Faculty of Medicine, University of Medical Sciences, Mashhad, Iran Abdoreza Shakeri Department of Pediatrics, Faculty of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran Shabnam Shahsavand Department of Molecular Sciences, Faculty of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran 2015 10 16 Febrile convulsion (FC) is the most common type of seizure in childhood that occurs in 2-5 % of the children younger than 6 years. Interleukin 1β (IL-1β) is a cytokine that contributes to febrile inflammatory responses. There are conflicting results on increasing this cytokine in serum during FC. Thus we measured IL-1ß in febrile children with or without seizure. 60 febrile children (6 months to 5 years old) were divided in two groups, one group consisted of 30 children with FC, the other group consisting of 30 children without seizure which served as control. Blood samples were collected from members of both groups and serum samples were prepared. Interleukin 1β concentrations were measured using a commercial Enzyme-linked immunosorbent assay (ELISA) kit. We found that there was a difference in serum levels of Interleukin 1β between FC and control group but it was not significant. This result may be due to the low number of samples or the result of Interleukin 1β binding to some large proteins such as α2- macroglobolin, complement and soluble type 2 Interleukin 1 receptor, that affected the free Interleukin 1β concentration. We could not find a significant relationship between serum Interleukin 1β concentration and FC. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/543 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/543/507

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 11 4 2012 12 15 340 344 EN Mohsen Badalzadeh Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Fatemeh Fattahi Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Mohammad Reza Fazlollahi Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Shaghayegh Tajik Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Mohammad Hassan Bemanian Department of Pediatrics, Division of Allergy and Immunology, Shahid Sadoughi Hospital, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Fatemeh Behmanesh Allergy Research Center, Ghaem Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Massoud Movahedi Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran AND Department of Immunology and Allergy, Children Medical Center, Tehran University of Medical Sciences, Tehran, Iran Massoud Houshmand Department of Medical Genetics, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran Zahra Pourpak Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran AND Department of Immunology and Allergy, Children Medical Center, Tehran University of Medical Sciences, Tehran, Iran 2015 10 16 Chronic granulomatous disease (CGD), a rare inherited primary immunodeficiency disorder,  is  caused  by  mutation  in  any  one  of  the  genes  encoding  components   of nicotinamide adenine dinucleotide phosphate (NADPH)-oxidase enzyme. NCF2 gene (encoding P67-phox component) is one of them and its mutation is less common to cause CGD (around 5-6%). Here, we assessed mutation analysis of NCF2 in 4 CGD patients with p67-phox defect in Iran. These patients showed classical CGD symptoms. NCF2 sequence analyses revealed two different homozygous mutations including a nonsense mutation in exon 4, c.304C>T (Arg102X) in one case and a CA deletion in exon 13 (Leu346fsX380) in one brother and sister;the latter is a new mutation which has not been reported in previous studies. In another patient in whom the attempts to amplify exon 2 individually from genomic DNA  were unsuccessful, PCR amplification of exon 2 revealed no band of this exon on agarose gel. A PCR amplification mix of  exon  2 and exon 7, with an internal control, confirmed the lack of exon 2 in this patient. Although a gross deletion in other exons of NCF2  has been previously reported, a large deletion encompassing exon 2 has been not reported yet. This abstract was also presented in ESID 2012, Florence, Italy. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/542 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/542/508

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 11 4 2012 12 15 345 348 EN Sepideh Safaei Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Mohammad Reza Fazlollahi Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Masoud Houshmand National Institute of Genetic Engineering Biotechnology Tehran, Iran Amir Ali Hamidieh Hematology oncology & Stem cell Transplantation research center, Tehran University of Medical Sciences, Tehran, Iran Mohammad Hassan Bemanian Department of pediatrics , Division of Allergy and clinical Immunology, Shahid Sadoghi Hospital, Shahid Sadoghi University of Medical Sciences, Yazd, Iran Samin Alavi Department of Pediatric Hematology-Oncology, Mofid Children's Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran Farideh Mousavi Department of Pediatrics, Shohada Center Hospital, Shaheed Beheshti University of Medical Sciences, Tehran, Iran Zahra Pourpak Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Mostafa Moin Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran 2015 10 16 Wiskott-Aldrich syndrome (WAS) is a life-threatening X-linked recessive immunodeficiency disease described as a clinical triad of thrombocytopenia,  eczema, and recurrent infections, caused by mutations of the WAS protein (WASP) gene. The milder form of this disease is X-Linked thrombocytopenia  (XLT) that  presents only as platelet abnormalities. Mutation analysis for 15 boys with Wiskott-Aldrich syndrome was performed. Five previously reported mutations and six novel mutations including G8X, R41X, D283E, P412fsX446, E464X, and AfsX358 were detected. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/541 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/541/509

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 11 4 2012 12 15 349 350 EN Amin Saburi Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Majid Shohrati Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Reza Karbasi-Afshar Department of Cardiovascular Diseases, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran 2015 10 16 EDITORIAL https://ijaai.tums.ac.ir/index.php/ijaai/article/view/540 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/540/510