Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 12 1 2013 03 15 1 17 EN Behzad Yeganeh Department of Physiology, University of Manitoba, Winnipeg, Manitoba, Canada AND Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, Manitoba, Canada AND Manitoba Institute of Child Heath, University of Manitoba, Winnipeg, Manitoba, Canada Adel Rezaei Moghadam Young Researchers Club, Ardabil Branch, Islamic Azad University, Ardabil, Iran Ahn Thuy Tran Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, Manitoba, Canada Mohammad Niaz Rahim Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, Manitoba, Canada Sudu R Ande Department of Internal Medicine, University of Manitoba, Winnipeg, Manitoba, Canada Mohammad Hashemi Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran Kevin M Coombs Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, Manitoba, Canada AND Manitoba Institute of Child Heath, University of Manitoba, Winnipeg, Manitoba, Canada Saeid Ghavami Department of Physiology, University of Manitoba, Winnipeg, Manitoba, Canada AND Manitoba Institute of Child Heath, University of Manitoba, Winnipeg, Manitoba, Canada AND St. Boniface Research Centre, University of Manitoba, Winnipeg, Manitoba, Canada 2015 10 16 Asthma is one of the fastest growing syndromes in many countries and is adding a huge cost to the health care system. Increasing reports have linked airway infectious diseases to asthma. Influenza is one of the most serious airway infectious diseases and in recent years there have been some serious influenza virus pandemics which caused increased fatality in numerous different populations. Diverse host response pathways during virus infection have been identified, including different cell death and survival pathways. These pathways include 1) programmed cell death I (apoptosis), 2) programmed cell death II (autophagy), and 3) endoplasmic reticulum stress with subsequent unfolded protein response (UPR). There has been extensive research on the regulatory roles of these pathways during the influenza virus life cycle. These studies address the benefits of enhancing or inhibiting these pathways on viral replication. Here we review the most recent and significant knowledge in this area for possible  benefits  to  clinicians and  basic  scientist researchers  in  different  areas  of  the respiratory and virology sciences. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/539 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/539/463

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 12 1 2013 03 15 18 28 Ali Soleimani Research Institute of Physiology and Biotechnology of Agriculture, University of Zanjan, Zanjan, Iran Sonia Morales Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Granada, Spain AND Proteomic Research Service, Hospital Universitario San Cecilio, Granada, Spain Jose Carlos Jimenez-Lopez Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Granada, Spain Antonio Jesús Castro Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Granada, Spain María Isabel Rodríguez-García Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Granada, Spain Juan de Dios Alché Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Granada, Spain 2015 10 16 Molecular evidence on the heterogeneity present in the Ole e 1 allergen of the olive pollen is emerging. Such polymorphism is dependent on the cultivar origin of pollen, which also determines wide differences in the expression of this protein. Determination of biochemical and molecular characteristics of Ole e 1 pollen allergen in two Iranian olive cultivars, namely 'Rowghani' and 'Zard' is necessary to assess their allergenicity potential. SDS-PAGE and immunoblotting analysis of pollen extracts showed that both cultivars present high and low expression of Ole e 1, respectively. These protein levels correlated with similarly different levels of transcripts, as determined by RT-PCR. Two-dimensional protein profiles also showed conspicuous differences in the distribution and the level of expression of those spots reacting to an anti-Ole e 1 antibody. Bioinformatic analysis of four Ole e 1 sequences corresponding to 'Rowghani' and two sequences for 'Zard', showed numerous heterogeneities when compared with those Ole e 1 and Ole e 1-like sequences present in databases. Nucleotide substitutions resulted in many cases in changes over the predicted amino acid sequences. A cladistic analysis of the sequences showed Iranian entries in a central position between West-European sequences, and Ole e 1-like sequences from other Oleaceae species. Moreover, amino acid changes affected key epitopes of the protein involved in the recognition of the protein by the human immune system. Putative implications of polymorphism in both the biological role and the allergic reactivity of Ole e 1 are discussed. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/538 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/538/464

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 12 1 2013 03 15 29 36 EN Tarak Dhaouadi Laboratory of Research in Immunology of Renal Transplantation and Immunopathology, Charles Nicolle Hospital, Tunis El Manar University, Tunis, Tunisia Imen Sfar Laboratory of Research in Immunology of Renal Transplantation and Immunopathology, Charles Nicolle Hospital, Tunis El Manar University, Tunis, Tunisia Hajer Aounallah-Skhiri National Institute of Public Health, Tunis, Tunisia Saloua Jendoubi-Ayed Laboratory of Research in Immunology of Renal Transplantation and Immunopathology, Charles Nicolle Hospital, Tunis El Manar University, Tunis, Tunisia Hend Bouacha Pneumonology Department, Charles Nicolle Hospital, Tunis, Tunisia Taieb Ben Abdallah Laboratory of Research in Immunology of Renal Transplantation and Immunopathology, Charles Nicolle Hospital, Tunis El Manar University, Tunis, Tunisia Yousr Gorgi Laboratory of Research in Immunology of Renal Transplantation and Immunopathology, Charles Nicolle Hospital, Tunis El Manar University, Tunis, Tunisia 2015 10 16 Chemokines and their receptors  play an important  role in the  late inflammatory stage of asthma. In this study, we aimed to investigate polymorphisms of MCP-1 (CCL2), CCR2 and CCR5 which can affect qualitatively and/or quantitatively their production and thus influence both susceptibility and severity of asthma and its clinical and biological features. MCP-1   (A/G   -2518),  CCR2   (+/64I),   CCR5   (G/A   -59029)  and   CCR5   (∆32)polymorphisms were evaluated by PCR in 107 Tunisian patients with asthma and 169 healthy controls. No  significant association  was  found  between  the  four  investigated  polymorphisms and   asthma.   Nevertheless   the   haplotype   MCP1*AG/CCR2*+/+   was  significantly  l ess frequent in patients (20.5%) compared to controls (32.5%) (p=0.03; OR=0.54; 95% CI:0.29-0.98). Whereas  no  difference  was  observed  in  CCR2/CCR5   haplotypes  between patients and controls. Analysis of polymorphisms with clinical and biological features showed that   the   concomitant   presence  of   MCP-1*G/CCR2*64I  alleles  was  less  frequent   in severe forms  (4.34%) compared  to  moderate  disease (12%) but  the  difference was not significant (p=0.27). No association was observed between the four polymorphisms and the presence of atopic rhinitis or atopic conjunctivitis and an elevated rate of serum IgE over 200 IU/ml. Additional effects of MCP-1 and its receptor CCR2 polymorphisms seem to be involved in disease susceptibility to  asthma in Tunisian patients; nevertheless they could be protective against its severe forms. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/537 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/537/467

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 12 1 2013 03 15 37 49 EN Mohsen Saeidi Department of Immunology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran AND Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Ahmad Masoud Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Yadollah Shakiba Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Jamshid Hadjati Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Mandana Mohyeddin Bonab Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Mohammad Hossein Nicknam Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran AND Molecular Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran Mostafa Latifpour Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Behrooz Nikbin Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran AND Molecular Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran 2015 10 16 The Wharton’s jelly of the umbilical cord is believed to be a source of mesenchymal stem cells (MSCs) which can be therapeutically applied in degenerative diseases. In this study, we investigated the immunomodulatory effect of umbilical cord derived- mesenchymal stem  cells (UC-MSCs) and  bone  marrow-derived-mesenchymal stem  cells (BM-MSCs) on differentiation, maturation, and endocytosis of monocyte-derived dendritic cells in a transwell culture system under laboratory conditions. Monocytes were differentiated into immature dendritic cells (iDCs) in the presence of GM-CSF and IL-4 for 6 days and then differentiated into mature dendritic cells (mDCs) in the presence of TNF-α for 2 days. In every stage of differentiation, immature and mature dendritic cells were separately co- cultured with UC-MSCs and BM-MSCs. The findings showed that UC-MSCs and BM-MSCs inhibited strongly differentiation and maturation of dendritic cells at higher dilution ratios (1:1). The BM-MSCs and UC-MSCs showed more inhibitory effect on CD1a, CD83, CD86 expression, and dendritic cell endocytic activity, respectively. On the other hand, these cells severely up-regulated CD14 marker expression.  We concluded that UC-MSCs and BM-MSCs could inhibit differentiation, maturation and endocytosis in monocyte-derived DCs through the secreted factors and free of any cell- cell contacts  under  laboratory conditions. As DCs  are believed to  be the  main antigen presenting cells for naïve T cells in triggering immune responses, it would be logical that their inhibitory effect on differentiation, maturation and function can decrease or modulate immune and inflammatory responses. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/536 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/536/468

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 12 1 2013 03 15 50 56 EN Mohammad Saeid Rahiminejad Department of Pediatrics, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran Mehrdad Mirmohammad Sadeghi Department of Pediatrics, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran Payam Mohammadinejad Research Center for Immunodeficiencies, Tehran University of Medical Sciences, Tehran, Iran Bamdad Sadeghi Research Center for Immunodeficiencies, Tehran University of Medical Sciences, Tehran, Iran Hassan Abolhassani Research Center for Immunodeficiencies, Tehran University of Medical Sciences, Tehran, Iran Mohammad Mehdi Dehghani Firoozabadi Research Center for Immunodeficiencies, Tehran University of Medical Sciences, Tehran, Iran Seyed Mohammad Fathi Department of Pediatrics, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran AND Research Center for Immunodeficiencies, Tehran University of Medical Sciences, Tehran, Iran Hamid Rezvani Department of Pediatrics, Taleghani Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran Gholamreza Bahoush Department of Pediatrics, Aliasghar Children's Hospital, Tehran University of Medical Sciences, Tehran, Iran Mohammad Ali Ehsani Department of Pediatrics, Bahrami Children's Hospital, Tehran University of Medical Sciences, Tehran, Iran Mohammad Faranoush Department of Pediatrics, MAHAK Children's Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran Azim Mehrvar Department of Pediatrics, MAHAK Children's Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran Babak Torabi Sagvand Research Center for Immunodeficiencies, Tehran University of Medical Sciences, Tehran, Iran Mojtaba Ghadiani Department of Pediatrics, Taleghani Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran Nima Rezaei Department of Pediatrics, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran AND Research Center for Immunodeficiencies, Tehran University of Medical Sciences, Tehran, Iran Asghar Aghamohammadi Department of Pediatrics, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran AND Research Center for Immunodeficiencies, Tehran University of Medical Sciences, Tehran, Iran 2015 10 16 Coincidence of autoimmune diseases such as immune thrombocytopenic purpura (ITP) with  immunodeficiencies has  been  reported  previously in  patients  who  suffered  from primary antibody deficiency (PAD). But there is no original study on immunological profiles of ITP patients to find out their probable immune deficiency. In this case-control study, ITP patients’ humoral immunity was investigated for diagnosis of PAD in comparison with normal population. To evaluate the humoral immune system against polysaccharide antigens, patients’ serum immunoglobulin levels were measured and a 23-valent pneumococcal  capsular polysaccharide vaccine (PPV23) was administrated  to evaluate the antibody response to vaccination. In  this  study, 14 out  of  36 patients  (39%) were diagnosed with antibody mediated immune deficiency including 2 patients (5.5%) with immunoglobulin class deficiency and 4 (11%) with IgG subclass deficiency. The remaining patients suffered from specific antibody deficiency. The most frequent deficiency in ITP patients was specific antibody deficiency. Therefore, immunological survey on ITP patients may be important especially for those who have undergone splenectomy. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/535 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/535/469

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 12 1 2013 03 15 57 62 EN Maryam Azimi Mohamadabadi Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran Zuhair Muhammad Hassan Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran Ahmad Zavaran Hosseini Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran Shekoofe Noori Department of Biochemistry, School of Medical Sciences, Shahid Beheshti University, Tehran, Iran Mehdi Mahdavi Department of Virology, Pasteur Institute of Iran, Tehran, Iran Saman Maroufizadeh Department of Biostatistics, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran Hamidreza Maroof Department of Microbiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran 2015 10 16 Recent studies have indicated the profound  anti-tumor activity of artemisinin's compounds,  among  which;  arteether  is an  oil-soluble derivative of  artemisinin with an endoperoxide bridge that can induce apoptosis in tumor cells but not in the normal cells. An experiment was carried out on tumor-bearing Balb/c mice to estimate the effects of Arteether  on  tumor  growth  and  antitumor  immune  responses.  Briefly, 6mg/kg/day  of Arteether and diluents were administered to two groups of mice. Tumor sizes were measured using  digital verniercallipers. Mice were  sacrificed and  splenocytes  were  harvested  for lymphocyte proliferation assay, the level of IL-4 and IFN-γ cytokines, and the percentage of splenic T regulatory cells were measured. According to the findings, there were no statistical differences between the groups with respect to the level of IFN-γ, IL-4 and proliferation assay; while our results showed that Arteether is effective in the reduction of tumor growth rate. In general, intra-tumoral injection of Arteether as an oil-soluble derivative of artemisinin brings to light some antitumor properties that may aid in development of more effective antitumor agents. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/534 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/534/470

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 12 1 2013 03 15 63 67 EN Parisa Bozorgzad Tehran University of Medical Sciences, Faculty of Nursing and Midwifery, Tehran, Iran Abbas Ebadi Behavioral Sciences Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. Mostafa Moin Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Forough Sarhangy Behavioral Sciences Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Somayyeh Nasiripour Department of Pharmacotherapy,  Tehran University of Medical Sciences, Tehran, Iran Mohammad Ali Soleimani Tehran University of Medical Sciences, Faculty of Nursing and Midwifery, Tehran, Iran 2015 10 16 One of the most important goals in treating chronic diseases, especially asthma, is the promotion of quality of life (QOL). The present study aimed at identifying the effect of educating method on the QOL of children, who suffered from asthma.In this clinical trial study, 80 children aged 6-12 years with asthma were divided into two groups: control (face-to-face method) and experimental (visual concept mapping method). The QOL of both groups was measured before and after educating by the Juniper's Quality of Life Questionnaire with a one-month interval.The rate of change in the QOL score of the experimental group was 0.3 (±0.7) both before and after intervention and 0.1 (±0.3) in the control group, which was statistically significant (p<0.05).Educating by the visual concept mapping method with regard to the manner of using sprays is taken into account as an efficient and effective method in improving the QOL of children with asthma. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/533 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/533/471

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 12 1 2013 03 15 68 74 EN Mozafar Mohammadi Nejad Razi Herbal Medicine Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran Eisa Salehi Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Mehrnaz Mesdaghi Department of Immunology, Mofid Children Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran Lida Atarod Vali-Asr Hospital, Tehran University of Medical Sciences, Tehran, Iran Masoud Movahedi Children Medical Center, Tehran University of Medical Sciences, Tehran, Iran Zahra Gheflati Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Tahereh Aboufazeli Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Mohammad Vodjgani Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran 2015 10 16 Allergic rhinitis  (AR) is  an  inflammatory  disorder  of  the  nasal  mucosa  with  high morbidity and prevalence. Natural killer (NK) cells might have a role in AR. We aimed to evaluate the changes of the markers and receptors on NK cells in AR patients compared to the non-atopic controls. Flow  cytometric  analysis was  used  with  double  staining  of  the  Peripheral  Blood Mononuclear Cells (PBMCs) to examine the expression of CD25 and CD69 markers, and NKG2D  and NKG2A  receptors on NK  cells of 20 patients with AR and 20 non-atopic controls. The serum total IgE level was measured by Enzyme-linked Immunosorbent Assay. The expression of CD69 antigen on NK cells in AR patients was significantly higher than that  of  healthy group  (p=0.03). No  significant changes were observed  between  CD25, NKG2D  and NKG2A expression on the surface of NK cells from healthy and AR subjects. Our study also showed that there was no significant correlation between the expression of CD69, CD25, NKG2D and NKG2A and level of serum total IgE in AR patients and normal subjects. These results indicated that the expression of CD69 antigen on NK cells of AR patients was increased. The high expression of CD69 on NK cells in AR patients suggested that these cells were activated, probably due to the cytokines secreted from allergen-stimulated T cells and activated monocytes. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/532 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/532/472

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 12 1 2013 03 15 75 80 EN Ehsan Darabi Department of Peridontotics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran Zeinab Kadkhoda Department of Peridontotics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran Aliakbar Amirzargar Molecular Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran 2015 10 16 Peri-implantitis is a multi-factorial disease involving peri-implant tissues and resulting in therapeutic failure. Inflammatory mediators and cytokines in the Gingival Crevicular Fluid (GCF)  have  pivotal  roles  in  the  disease pathogenesis  and  could  be  used  for  disease monitoring. Therefore, the  present  study was conducted  to  compare the GCF  levels of TNF-α and IL-17 between patients with peri-implantitis and healthy implants. In this case-control study, 24 patients with peri-implantitis and 18 individuals with healthy implants referring to  faculty of dentistry in Tehran  University of Medical Sciences were selected. GCF was collected by paper cons number 30. Samples were preserved in PBS -70ºC. TNF-α  and IL-17 levels in GCF  were determined by ELISA method.  Data  were analyzed by SPSS software version 13, using descriptive indices and independent t tests. Mean probing depth in peri-implantitis and control groups were 6.2 ± 1.1 and 3.7 ± 1.6 mm respectively. Mean level of IL-17 in patients with peri-implantitis was significantly more than the control group (19.8 ± 16.0 versus 9.3 ± 8.4 pg per site in 40 seconds, p=0.016). Also, mean level of TNF-α in patients with peri-implantitis was more than control group (39.0 ± 3.9 versus 14.5 ± 9.0 pg per site in 40 seconds, p =0.000). The  significant higher  levels of  TNF-α  and  IL-17  in  patients  with  peri-implantitis compared to control group indicated the pivotal role of these cytokines in peri-implantitis and could be suggested as diagnostic markers and in future possibly for immunomodulatory treatments. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/531 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/531/473

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 12 1 2013 03 15 81 85 EN Fatemeh Hajari Taheri Department of Immunology, Hybridoma Lab, Pasteur Institute of Iran, Tehran, Iran AND Faculty of Science, Islamic Azad University, Science and Research Branch, Tehran, Iran Maryam Seyedolmohadesin Department of Immunology, Hybridoma Lab, Pasteur Institute of Iran, Tehran, Iran AND Faculty of Science, Islamic Azad University, Science and Research Branch, Tehran, Iran Mansour Bayat Department of Medical and Veterinary Mycology, Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran Mehdi Mahdavi Department of Virology, Pasteur Institute of Iran, Tehran, Iran Mohammad Hossein Yazdi Department of Pharmaceutical Biotechnology, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran Ali Eslamifar Clinical Lab, Pasteur Institute of Iran, Tehran, Iran Mohsen Abolhasani Department of Immunology, Hybridoma Lab, Pasteur Institute of Iran, Tehran, Iran 2015 10 16 Breast cancer  patients  are  susceptible to  infections such  as  candidiasis. Due  to  the importance and the role of matrix metalloproteinases (MMP) in breast cancer progression and its correlation with tumor metastasis, we analyzed the serum level of MMPs -2, -3, -9 and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) in breast cancer bearing mice in the presence of systemic Candida albicans infection. Female BALB/c  mice were divided into 4 groups:  group I had tumor  +  candidiasis; group II, tumor only; group III, candidiasis only and group IV as negative control. Tumor tissue was separated from stock breast cancer bearing mice and transplanted subcutaneously into the groups I and II mice. Two weeks after tumor transplantation, groups I and III were infected with Candida albicans by intravenous injection. One week after systemic infection, the sera of the experimental groups were prepared and analyzed with ELISA for MMP-2, -3, -9 and TIMP-1 levels. The results showed that the levels of MMP-3, MMP-9 and TIMP-1 were increased in groups I, II and III, as compared to the control group. However, the level of MMP-2 was decreased in mice infected with Candida albicans and in infected mice bearing tumor. These data suggest that candidiasis may have a positive effect on tumor progression and metastasis. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/530 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/530/474

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 12 1 2013 03 15 86 92 EN Zahra Alizadeh Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Mohammad Reza Fazlollahi Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Massoud Houshmand National Institutes for Genetics Engineering and Biotechnology, Tehran, Iran Marzieh Maddah Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Zahra Chavoshzadeh Pediatric Infectious Research Center, Mofid Children Hospital, Shahid Beheshti Medical University, Tehran, Iran Amir Ali Hamidieh Hematology, Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran Bibi Shahin Shamsian Pediatric Congenital Hematologic Disorders Research Center, Shahid Beheshti University of Medical Sciences.Tehran, Iran Payman Eshghi Pediatric Congenital Hematologic Disorders Research Center, Shahid Beheshti University of Medical Sciences.Tehran, Iran Samaneh Bolandghamat Pour Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Hoda Sadaaie Jahromi Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Mahboobeh Mansouri Department of Immunology and Allergy, Mofid Children Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran Masoud Movahedi Department of Allergy and Clinical Immunology, Children’s Medical Center, Tehran University of Medical Sciences, Tehran, Iran Mohsen Nayebpour Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran Zahra Pourpak Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran AND Department of Allergy and Clinical Immunology, Children’s Medical Center, Tehran University of Medical Sciences, Tehran, Iran Mostafa Moin Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran AND Department of Allergy and Clinical Immunology, Children’s Medical Center, Tehran University of Medical Sciences, Tehran, Iran 2015 10 16 Severe  congenital  neutropenia  (SCN)  is  a  rare  primary  immunodeficiency   disease. Different genes are found to be associated with SCN, including ELA2, HAX1, WAS, GFI1, G-CSFR  and  G6PC3.  The  aim  of  this  study  was  to  find  different  gene  mutations responsible for SCN in Iranian patients. Twenty-seven   patients   with   SCN  referred   to  Immunology,   Asthma   and  Allergy Research  Institute  during  a  five  year  priod  5  years  (May  2007  and  May  2012),  were included  in this study. Neutropenia  related  exons and flanking  regions  of ELA2,  HAX1, WAS,  GFI1,  G-CSFR  and  G6PC3  were  amplified  by  PCR  and  the  sequences  were analyzed. The  results  showed  different  mutations  including  4  ELANE  mutations,  11  HAX1 mutations and 2 G6PC3 mutations. None of the patients had GFI1 mutation and also one mutation  was  found  in  G-CSFR  in  a patient  with  ELANE  mutation.  Ten  patients  had unknown genetic diagnosis which was compatible with other studies. According to these results, most of the patients showed HAX1 mutations and this finding which significantly  differed from other reports, might be related to differences  in Iranian ethnicity and also in high rate of consanguineous marriages in Iran. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/529 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/529/475

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 12 1 2013 03 15 93 95 EN Hamid Ahanchian Allergy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran AND Queensland Children's Medical Research Institute, University of Queensland, Brisbane, Australia Reza Farid Allergy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Elham Ansari Allergy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Hamid Reza Kianifar Allergy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Farahzad Jabbari Azad Allergy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Seyed Ali Jafari Department of Gasroenterology, Mashhad University of Medical Sciences, Mashhad, Iran Reza Purreza Allergy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Shadi Noorizadeh Department of Gasroenterology, Mashhad University of Medical Sciences, Mashhad, Iran 2015 10 16 Food Dependent Exercise-Induced Allergy is a rare condition. However, the occurrence of anaphylaxis is increasing especially in young people. The diagnosis of anaphylaxis is based on clinical criteria and can be supported  by laboratory tests such as serum tryptase and positive skin test  results  for  specific IgE  to  potential  triggering allergens. Anaphylaxis prevention needs strict avoidance of confirmed relevant allergen.  Food-exercise challenge test  may be  an acceptable method  for  diagnosis of  Food  ependent  Exercise-Induced Allergy and dietary elimination of food is recommended to manage it. In this study, a 32 year-old woman visited the allergy clinic with a history of several episodes of hives since 11 years ago and 3 life-threatening attacks of anaphylaxis during the previous 6 months. The onsets of majority of these attacks were due to physical activity after breakfast. On Blood RAST test, the panel of common food Allergens was used and she had positive test only to wheat flour. On skin prick tests for common food allergens she showed a 6 millimeter wheal with 14 mm flare to Wheat Extract. The rest of allergens were negative. The patient was diagnosed as wheat-dependent exercise-induced, and all foods containing wheat were omitted from her diet. In this report we emphasized on the importance of careful history taking in anaphylaxis diagnosis. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/528 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/528/476

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 12 1 2013 03 15 96 98 EN Behnaz Esmaeili Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Mohammad Reza Fazlollahi Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Parisa Dashti Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Parvaneh Farzaneh Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Zahra Pourpak Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran AND Department of Allergy and Clinical Immunology, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran Mostafa Moin Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran AND Department of Allergy and Clinical Immunology, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran 2015 10 16 LETTER TO THE EDITOR https://ijaai.tums.ac.ir/index.php/ijaai/article/view/527 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/527/477