Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 12 2 2013 06 15 99 106 EN Maryam Sharif Shoushtari Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran AND Department of Biology, Faculty of Biology Science, Islamic Azad University, North Tehran Branch, Iran Ahmad Majd Department of Biology, Faculty of Biology Science, Islamic Azad University, North Tehran Branch, Iran Zahra Pourpak Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran AND Department of Immunology and Allergy, Children Medical Center, Tehran University of Medical Sciences, Tehran, Iran Youcef Shahali Laboratoire “Sciences Analytiques, Bioanalytiques et Miniaturisation”, ESPCI ParisTech, UMR 7195 CNRS, 75231 Paris cedex 05, France Mostafa Moin Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran AND Department of Immunology and Allergy, Children Medical Center, Tehran University of Medical Sciences, Tehran, Iran Mohammad Bagger Eslami Department of Pathobiology, Immunology Division, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran 2015 10 16 Weed pollen grains belonging to  the  Asteraceae  family contain  a variety of  allergens inducing type I  and IV allergies in susceptible people. The  aim of this research was to compare  the  allergenic properties  of  immature  and  mature  Shasta  daisy pollen  grains (Chrysanthemum maximum Ramond) to define the potential role of the maturation process on the allergenicity of Asteraceae pollen grains. The  immature (IP) and mature  pollen (MP) grains were first studied by optical and scanning electron microscopand their protein contents were quantitatively and qualitatively analyzed. Pollen extracts were finally used to sensitize guinea pigs in order to obtain IP and MP specific antibodies. Nasal provocation tests using IP and MP crude extracts were also performed on pre-sensitized guinea pigs. The  MP  extract  induced  IgE  and  eosinophilia in  blood  and  positive skin  tests  in sensitized guinea pigs. Moreover, high number of eosinophils was found in the nasal mucosa of MP sensitized guinea pigs. SDS-PAGE analysis of the IP and MP protein content showed seven and five apparent bands ranging from 7 to 66kDa respectively. According to immunoblot analysis, MP extract contained a single allergen of 66kDa. The overall results showed developmental processes of Shasta daisy pollen grains towards both morphological and molecular changes increasing their allergenic potency. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/526 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/526/479

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 12 2 2013 06 15 107 114 EN Nevin Uzuner Dokuz Eylul University Hospital, Department of Pediatric Allergy, 35340, Balcova, Izmir, Turkey. Arzu Babayigit Hocaoglu Dokuz Eylul University Hospital, Department of Pediatric Allergy, 35340, Balcova, Izmir, Turkey. Duygu Olmez Erge Dokuz Eylul University Hospital, Department of Pediatric Allergy, 35340, Balcova, Izmir, Turkey. Suna Asilsoy Baskent University Hospital, Department of Pediatric Allergy, Adana, Turkey Ozkan Karaman Dokuz Eylul University Hospital, Department of Pediatric Allergy, 35340, Balcova, Izmir, Turkey. Zekiye Altun Dokuz Eylul University Hospital, Department of Biochemistry, 35340, Balcova, Izmir, Turkey Abdullah Kumral Dokuz Eylul University Hospital, Department of Neonatology, 35340, Balcova, Izmir, Turkey Ozlem Tufekci Dokuz Eylul University Hospital, Department of Pediatric Allergy, 35340, Balcova, Izmir, Turkey. Huray Islekel Dokuz Eylul University Hospital, Department of Biochemistry, 35340, Balcova, Izmir, Turkey 2015 10 16 The identification of early markers of atopy in cord blood of newborns at delivery may offer prediction of future allergic sensitization. The aim of this study was to evaluate the relationship between cord blood interleukin-13 (IL-13) and interferon-gamma (IFN-γ) and development of allergic diseases during the first five years of life. Umbilical cord blood samples were collected at the time of delivery from 62 newborns. The families of these newborns were asked to complete a questionnaire about age and education of parents, number of siblings, allergic diseases in family members, cigarette exposure during pregnancy and presence of pets in their house. The same subjects were evaluated when they were five years old. Venous blood samples were drawn and epidermal skin prick tests were performed. IL-13 and interferon-gamma (IFN-γ) levels were studied from the blood samples which were taken during birth and five years later. There was no significant relationship between gender, type of delivery, educational levels of parents, exposure to cigarette smoke, atopy in parents, presence of pets in the house and IL-13 and IFN-γ levels in cord blood and at five years. Higher levels of IL-13 in newborns and five years olds, were found significantly related to skin prick test positivity (p=0.004 and p<0.0001, respectively) and presence of allergic diseases (p= 0.008 and p= 0.001, respectively). Levels of IFN-γ, both in cord blood and five years after, were not related with the future of allergic status of children. Higher levels of IL-13 in cord blood may be a predictor of future development of allergic sensitization. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/525 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/525/483

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 12 2 2013 06 15 115 123 EN María Morales Suárez-Varela Public and Environmental Health Unit, Department of Preventive Medicine, University of Valencia, CIBER Epidemiology and Public Health (CIBERESP), and Center for Public Health Research (CSISP), Valencia, Spain Luís García-Marcos Pediatric Respiratory and Allergy Units, “Arrixaca” University Children’s Hospital, University of Murcia, and CIBER of Epidemiology and Public Health (CIBERSP), Murcia, Spain Javier Fuertes Fernández-Espinar Perpetuo Socorro” Health Center, Huesca, Spain Alberto Bercedo-Sanz Meruelo” Health Center, Santander, Spain Inés Aguinaga-Ontoso Department of Health Sciences, Public University of Navarra, Pamplona, Spain Carlos González-Díaz Department of Pediatrics, Basurto Hospital, Bilbao, Spain Ignacio Carvajal-Urueña La Ería” Health Center, Oviedo, Asturias Princedom Health Service, Asturias, Spain Rosa María Busquet-Monge Department of Pediatrics, Hospital del Mar, Barcelona, Spain Alberto Arnedo-Pena Centre of Public Health, Regional Health Authority, Castellón, Spain Nagore García de Andoin Department of Pediatrics, “Donostia” Hospital, San Sebastián, Guipuzcoa, Spain José Batlles-Garrido Department of Pediatrics, “Torrecárdenas” Hospital, Almería, Spain Alfredo Blanco-Quirós Department of Pediatrics, University of Valladolid, Spain Angel López-Silvarrey Varela María José Jove Foundation, La Coruña, Spain Gloria García-Hernández Pediatric Allergy and Pulmonology Unit. “12 de Octubre” University Children’s Hospital, Madrid, Spain Natalia Gimeno-Clemente Public and Environmental Health Unit, Department of Preventive Medicine, University of Valencia, CIBER Epidemiology and Public Health (CIBERESP), and Center for Public Health Research (CSISP), Valencia, Spain Nuria Rubio-López Public and Environmental Health Unit, Department of Preventive Medicine, University of Valencia, CIBER Epidemiology and Public Health (CIBERESP), and Center for Public Health Research (CSISP), Valencia, Spain Agustín Llopis-González Public and Environmental Health Unit, Department of Preventive Medicine, University of Valencia, CIBER Epidemiology and Public Health (CIBERESP), and Center for Public Health Research (CSISP), Valencia, Spain 2015 10 16 Atopic Eczema (AE) is a chronic inflammatory skin disease that affects children and adults, and  alters  quality  of  life.  Previous  studies  have suggested several  socio-demographic   and environmental factors related to the prevalence of AE and other allergic diseases,  including acetaminophen use. In the present study, we report the rates of isolated AE, AE associated with asthma and AE associated with rhinitis among 13- to 14-year-old Spanish adolescents and the level of association of these conditions with the use of acetaminophen. We analyzed Spanish data from a cross-sectional Phase 3 study within ISAAC. A total of 28,717 adolescents completed the Phase 3 written questionnaire by answering questions for acetaminophen use and on asthma, rhinitis and AE symptoms. We observed an association between acetaminophen use and AE among the adolescents who had used acetaminophen in the previous month. Furthermore, the prevalence rate increased with the number of allergic processes: for AE alone, the adjusted Prevalence Ratio (aPR) was 1.81 and for AE associated with rhinitis or with asthma, aPRs were 2.20 and 3.03, respectively. We conclude that acetaminophen use in childhood  may be an important factor associated with development and/or maintenance of AE and other allergic diseases. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/524 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/524/486

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 12 2 2013 06 15 124 128 EN Shideh Assar Department of Pediatrics, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Esmaeil Idani Departments of Respiratory Disease and Internal Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Mehdi Monajemzadeh Department of Pediatrics, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Masoome Ganai Department of Pediatrics, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Fakher Rahim Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran 2015 10 16 Asthma is the most common chronic disease in children. The natural history of asthma is often  characterized by periods  of  remission and  relapse. The  aim of  this study was to determine the remission rate of asthmatic children after entering the second decade of life and remission related factors in a hospital–based cohort. The  study  population  was  asthmatic  children  who  were  diagnosed,  registered  and followed up in outpatient pediatric clinic of a university hospital in Ahvaz, Iran. Remission was assessed in the age of 11 to 15 years-old if ≥ 5 years passed from the time of diagnosis. Clinical remission was defined as the absence of asthma symptoms for at least one year without the use of inhaled corticosteroids and short acting β2 agonists. In the cases with clinical remission, spirometry and exercise tests were conducted  to document complete remission. The study included 197 adolescents (mean age of 13.1±1.9 years). Clinical remissions were found in 71 cases (%36) but according to spirometry criteria,65 children (33%) were in complete remission. There was no significant difference between the genders. The remission rate was positively correlated with age of onset, and inversely correlated with family history of asthma, atopic dermatitis, hospitalization due to asthma, passive smoking and the need to use long-term inhaled corticosteroid. Based on this study, approximately one third of asthmatic children showed remission up to 15 years of age which is a moderate rate compared to other studies. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/523 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/523/492

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 12 2 2013 06 15 129 135 EN Alain Raimundo Rodriguez-Orozco Facultad de Ciencias Médicas y Biológicas “Dr Ignacio Chávez”. Universidad Michoacana de San Nicolás De Hidalgo. Instituto de Investigación Científica en temas de Familia, Alergia e Inmunología, Morelia, México Rosa María Núñez-Tapia Unidad de Medicina Familiar No 80 del Instituto Mexicano del Seguro Social, Morelia, México Armando Ramírez-Silva Unidad de Medicina Familiar No 80 del Instituto Mexicano del Seguro Social, Morelia, México Carlos Gómez-Alonso Centro de Investigaciones Biomédicas de Occidente. Instituto Mexicano del Seguro Social, Morelia, México 2015 10 16 Asthma has been linked to family disfunctioning and poor control of the disease. This study was conducted to analyze the interactions between the level of intermittent asthma control, family functioning and respiratory function and between quality of life of asthmatic patients and their caregivers. 7 to 15 years old children with intermittent asthma were included. Asthma Control Test Questionnaire,  Pediatric  Asthma  Quality  of  Life  Questionnaire   (PAQLQ)   test,  and flowmetry were applied  to  children  and  Pediatric  Asthma  Caregiver´s Quatily of  Life Questionnaire  (PAQCLQ)  and  the  Family Functioning  Perception  Test  (FF-SIL) were applied to their parents. The most affected areas of family functioning in dysfunctional families were adaptability and permeability. A medium  to  high strength  of  association was founded  between  the emotional function of parents and the emotional function of children, R2=0.552. The most remarkable associations were among parents’ limitation of activities and parents' emotional function (r=0.837), parents’ limitation of activities and child's emotional function (r=0.722), parents' emotional role and limitation of activities (r=0.837), parents’ emotional role and emotional functioning of children with asthma (r=0.743) and the limitation of activities of children with asthma and the emotional function of children with asthma (r=0.870). No  direct associations were founded  among respiratory function, disease control  and family functioning in Mexican children with intermittent asthma and emotional function of parents and children were associated in both groups. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/522 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/522/495

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 12 2 2013 06 15 136 143 EN Rana Keyhanmanesh Tuberculosis and Lung Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran Horeyeh Bagban Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran Hossein Nazemieh Research Center for Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran Fariba Mirzaei Bavil Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran Mohammad Reza Alipour Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran 2015 10 16 Our  previous  studies demonstrated  the  preventive effect  of  Nigella  sativa  extract  on asthma and water-soluble substances of this extract, especially its methanol fraction were responsible for this relaxation on contracted tracheal chains of guinea pigs. For the first time, the present study has been conducted to determine the main constituents of its methanolic extract. Four constituents of 20%-methanolic fraction, consisting of two flavonoids (20-20% and 21-20% fractions) and two polysaccharides (1-20% and 2-20% fractions), were purified by analytical and  preparative  HPLC.  The  relaxant  effects  of  four  cumulative  concentrations  of  each constituent (50, 100, 150 and 200 mg/lit) in comparison with saline (1 ml) as negative control and four cumulative concentrations of theophylline (0.2, 0.4, 0.6 and 0.8 mM) as positive control were examined on methacholine-precontracted guinea pig tracheal chains (n=6). All concentrations  of theophylline and most concentrations  of 20-20, 21-20 fractions showed significant relaxant effects compared to  that  of saline. 20-20 fraction (Comferol diglucoside) was the most potent bronchodilator. Their relaxant effects were lower than that of theophylline. Polysaccharides (1-20, 2-20 fractions) did not  have any relaxant effects compared to that of saline. These results revealed that two flavonoids of 20%-methanolic fraction of Nigella  sativa were its main relaxant constituents. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/521 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/521/497

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 12 2 2013 06 15 144 152 EN Monireh Sadat Mirzamani Genomics Division, Systems Biology Institute, Chemical Injury Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran AND Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran Mohammad Reza Nourani Genomics Division, Systems Biology Institute, Chemical Injury Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Abbas Ali Imani Fooladi Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Samad Zare Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran Majid Ebrahimi Genomics Division, Systems Biology Institute, Chemical Injury Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran AND Department of Organ Anatomy, Yamaguchi University Graduate School of Medicine, Ube, Japan Samaneh Yazdani Genomics Division, Systems Biology Institute, Chemical Injury Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran AND Department of Pathology, Tohoku University GraduateSchool of Medicine, Sendai, Japan Mostafa Ghanei Genomics Division, Systems Biology Institute, Chemical Injury Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Mohammad Hassan Karimfar Department of Anatomy, Medical School, Ilam University of Medical Sciences, Ilam, Iran 2015 10 16 The widespread use of sulfur mustard  (SM) as a chemical warfare agent in the  past century has proved its long-lasting toxic effects. Despite a lot of research over the past decades on Iranian veterans, there are still major gaps in the SM literature. Transforming growth  factor  (TGF-β),  a  cytokine  that  affects  many  different  cell processes,  has  an important role in the lungs of patients with some of chronic airway diseases, especially with respect to airway remodeling in mustard lung. Primary airway fibroblasts from epibronchial biopsies were cultured, and gene expression of TGF-β1, TGF-β2, TbR-I and TbR-II in fibroblasts of SM injured patients and controls were investigated. Expression of TGF-βs and receptors was measured by RT-PCR. Protein level of TGF-β1was surveyed by western blot. Our  findings revealed that expression levels of TGF-β1,  TGF-β2,  TbR-I and TbR-II were upregulated in the  airway fibroblasts of  SM exposed patients  in comparison  with control samples. TGF-β1 expression was shown to be markedly increased in primary lung fibroblasts of chemically injured patients. Our  novel data, suggested that  over-expression of TGF-β  molecule and receptors  in primary airway fibroblasts of mustard gas injured patients may be involved in progression of airway remodeling of these patients. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/520 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/520/500

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 12 2 2013 06 15 153 160 EN Leila Mirbagheri Genomics Division, Systems Biology Institute. Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran AND Biochemistry Department, Sciences and Technology Branch, Azad University, Tehran, Iran Mehryar Habibi Roudkenar Research Center, Iranian Blood Transfusion Organization, Tehran, Iran Abbas Ali Imani Fooladi Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Mostafa Ghanei Genomics Division, Systems Biology Institute. Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Mohammad Reza Nourani Genomics Division, Systems Biology Institute. Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran 2015 10 16 Sulfur mustard (SM) has been identified as an important chemical weapon. During the Iran-Iraq war of 1980-88, the extensive usage of SM against Iranian civilians and military forces was proven. This agent has been shown to cause severe damage mainly in the skin, eyes, lungs,  and  respiratory  tract  in  Iranian  veterans.  The  most  common  disease  is bronchiolitis obliterans (BO)). SM increases the endogenous production of reactive oxygen species (ROS). Superoxide dismutases (SODs) are known as protective antioxidants against the harmful effects of ROS. Twenty exposed SM individuals (43.2±6.4 years), and 10 normal controls (41.3±2.5 years) were enrolled in this study. Evaluation of SODs was performed by semiquantitative RT-PCR and mmunohistochemistry. Our  results  demonstrated  that  CuZnSOD   and  MnSOD  mRNA  were  up-regulated 2.79±1.09  and  2.49±1.11  folds, respectively in SM-injured patients  in comparison  with control  levels. In  contrast,  Immunohistochemistry  results  showed  downregulation  of CuZnSOD protein expression in SM injured patients. Our results revealed that SODs may play an important role in cellular protection against oxidative stress due to mustard gas toxicity in airway wall of SM exposed patients. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/519 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/519/503

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 12 2 2013 06 15 161 167 EN Nastaran Alizadeh Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Mohammad Mehdi Amiri Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Alireza Salek Moghadam Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Amir Hassan Zarnani Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Farshid Saadat Department of Immunology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran Farnaz Safavifar Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran Azar Berahmeh Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran Mohammad Reza Khorramizadeh Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran AND Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran AND Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran 2015 10 16 There exists compelling evidence that Toll-like receptor 3 (TLR3) agonists can directly affect human cancer cells. The aim of this study was to investigate anti-cancer effects of TLR3 agonist in human breast cell line. We assessed potential effects of poly (A:U) on human breast cell line (MDA-MB-231)  on a dose-response and time-course  basis. Human breast cell line  MDA-MB-231  was treated with different concentrations of poly (A:U) and lipopolysaccharide  (LPS). Then, the following assays were performed on the treated cells: dose-response  and time-course cytotoxicity using colorimetric method; matrix metalloproteinase-2  (MMP-2) activity using gelatin zymography method; apoptosis using annexin-v flowcytometry method; and relative expression of TLR3 and MMP-2 mRNA using reverse transcriptase  polymerase chain reaction (RT-PCR) method. Following treatments, dose- response and time-course cytotoxicity using a colorimetric method, (MMP-2) activity (using gelatin zymography), apoptosis  (using annexin-v flowcytometry method) assays and expression of TLR3 and MMP-2 genes (using PCR method) were performed. Cytotoxicity and flowcytometry analysis of poly (A:U) showed that poly (A:U) do not have any cytotoxic and apoptotic effects in different concentrations used. MMP-2 activity analysis showed significant decrease in higher concentrations  (50 and 100 µg/ ml) between treated and untreated cells.  Moreover, poly A:U treated cells demonstrated decreased expression of MMP-2 gene in higher concentrations. Collectively, our data indicated that human breast cancer cell line (MDA-MB-231) was highly responsive to poly (A:U). The antimetastatic effect of direct poly (A:U) and TLR3 interactions in MDA-MB-231 cells could provide new approaches in malignant tumor therapeutic strategy. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/518 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/518/514

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 12 2 2013 06 15 168 175 EN Fatemeh Esfahanian Department of Endocrinology, Imam Khomeini Hospital, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Effat Naimi Department of Endocrinology, Baqiyatallah University of Medical sciences, Tehran, Iran Farideh Doroodgar Eye Research Center, Imam Khomeini Hospital, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Zohre Jadali Department of Pathobiology, Immunology Division, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran 2015 10 16 About 25-50% of Graves' disease (GD) patients develop thyroid eye diseases, which is associated with inflammatory process and abnormalities in the levels of several cytokines in orbital tissues in GD.  The aim of this study was to determine the Th1 and Th2 serum cytokines in patients with GD with or without ophthalmopathy. Serum  levels of  cytokines  and  autoantibodies  including Interferon-gamma  (IFN-γ),Interleukin-2 (IL-2), Interleukin-4 (IL-4), Interleukin-10 (IL-10), TSH receptor autoantibody (TRAb), thyroid  peroxidase antibody (TPOAb)  and  thyroglobulin antibody(TgAb) were measured by enzyme linked immunosorbent assay(ELISA) in 34 patients with GD and in 33 normal controls. Patients were also divided in two subgroups: 18 cases with ophthalmopathy and 16 cases without ophthalmopathy. Cytokine and antibody responses were analyzed in both groups. Compared with control subjects, patients with GD  showed elevated levels of IL-2 and IL-10. IFN-γ  levels were lower in patients in comparison to the controls. No significant differences were found  between patients and controls regarding the IL-4. There was no statistically significant  difference  in  cytokine  levels  between  those   with  or   without ophthalmopathy. Quantitative-cytokine  analysis  demonstrated  that  a  combination  of  Th1  and  Th2 cytokines may contribute to the pathogenesis of GD. These results also indicate that IL-10, but not IL-4, is related to the moderate and severe forms of thyroid associated ophthalmophathy. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/517 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/517/515

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 12 2 2013 06 15 176 181 EN Hassan Abolhassani Research Center for Immunodeficiencies, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran Asghar Aghamohammadi Research Center for Immunodeficiencies, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran Sarvenaz Pourjabbar Research Center for Immunodeficiencies, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran Mohammad Salehi Sadaghiani Research Center for Immunodeficiencies, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran Sina Nikayin Research Center for Immunodeficiencies, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran Anahita Rabiee Research Center for Immunodeficiencies, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran Amir Imanzadeh Research Center for Immunodeficiencies, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran Javad Mahmmoodi Gharaei Department of Psychiatric, Rozbeh Hospital, Tehran University of Medical Sciences, Tehran, Iran Mohammad Arbabi Department of Psychiatric, Rozbeh Hospital, Tehran University of Medical Sciences, Tehran, Iran Nima Rezaei Research Center for Immunodeficiencies, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran AND Molecular Immunology Research Center; and Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran 2015 10 16 Primary immunodeficiency diseases (PID) consist of a group of long-term illnesses which had permanent psychiatric effects on the patients and their parents. This study was designed to find out the most important origins and aspects of stressor in parents of PID patients.To assess the  impact of  psychiatric aspects in parents  of PID  patients, a valid and reliable questionnaire was compiled based on patients' complaints and consulting professionals in PID and psychology. Fathers of 26 PID patients (17 male and 9 female) were enrolled in this study. According to the result of this study, anxiety for long duration of disease of child (mean score= 4.42), anxiety for incurable diseases of child (mean score=4.23) and anxiety for side effects and complication of treatments on child (mean score=4.08) were the most important stressors of parents. The  comparison  between  specific PID  groups  showed  that  there  were  significant differences between total score of groups (XLA= 92.8±31.2, CVID=78.7±19.5 and other types of PID=  90.7±22.5, p-value =0.37). Survey for finding fundamental stressors and continuation of psychological counseling are necessary to achieve successful management of PID patients and their parents. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/516 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/516/517

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 12 2 2013 06 15 182 189 EN Parisa Karimi Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Seyedeh Zalfa Modarresi Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Mohammad Ali Sahraian Department of Neurology, Sina Hospital, Tehran University of Medical Sciences, Tehran, Iran Raheleh Shokouhi Shoormasti Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Maryam Mahlooji Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Anoushirvan Kazemnejad Department of Biostatistics, Tarbiat Modares University, Tehran, Iran Zahra Pourpak Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran 2015 10 16 Multiple Sclerosis (MS) and Allergy are believed to up regulate T helper cell type 1 (Th1) and T helper cell type 2 (Th2) responses, respectively. It has been shown that disequilibrium in the  ratio  of  Th1/Th2  activities may increase frequency of  one  disease and  decrease the frequency of the other. The aim of this study was to investigate the relation of MS with allergy and atopy in new diagnosed MS patients. This case-control study was conducted on 40 new diagnosed MS patients and the same number  of  normal controls. All of  the  patients were diagnosed (according to  McDonald criteria) at most 2 years prior to the study. Demographic data and clinical characteristics of both groups were recorded in a questionnaire. The total IgE and allergen specific IgE in the serum were measured in all the cases. Forty MS patients (female/male: 4.71) with mean age of 30.55±9.5 years and 40 healthy controls entered in this study. History of allergy was observed in 20(50%) of MS patients (including 15 (37.5%) rhinitis, 6 (15%) conjunctivitis, 3 (7.5%) urticaria and eczema, 1 (2.5%) asthma), and 20 (50%) of the controls (including 8 (20%) rhinitis, 4 (10%) conjunctivitis, 7 (17.5%) urticaria and eczema, 1 (2.5%) asthma). The differences between the two groups were not  statistically significant. Neither the serum total IgE, nor the frequency of specific IgE against Weed mix, Grass Mix, Tree mix1, Tree mix 2, Dermatophagoides Farinae, Dermatophagoides pteronyssinus and Epidermal and animal proteins mix differed statistically between the  two  groups. There  was also no  significant relationship between MS clinical manifestations and allergy prevalence and also between MS and atopy.  The results of this study as some other similar studies showed the same prevalence of allergy in MS patients and controls and also demonstrated no relation between MS and atopy. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/515 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/515/518

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 12 2 2013 06 15 190 191 EN Pegah Teymourpour Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Raheleh Shokouhi Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Mohammad Reza Fazlollahi Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Masoud Movahedi Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran AND Department of Immunology and Allergy, Children’s Medical Center, Tehran University of Medical Sciences, Tehran, Iran Mahboobeh Mansouri Pediatric Infections Research Center (PIRC), Mofid Children’s Hospital, Shaheed Beheshti University of Sciences, Tehran, Iran Bahram Mirsaeedghazi Department of Pediatric, Bahrami Hospital, Tehran University of Medical Sciences, Tehran, Iran Anoshirvan Kazemnejad Department of Biostatistics, Tarbiat Modares University, Tehran, Iran Seyedeh Kimia Yavari Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Zahra Pourpak Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Mostafa Moin Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran AND Department of Immunology and Allergy, Children’s Medical Center, Tehran University of Medical Sciences, Tehran, Iran 2015 10 16 No Abstract https://ijaai.tums.ac.ir/index.php/ijaai/article/view/514 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/514/519

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 12 2 2013 06 15 192 194 EN Amir Ghasemi Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Mohammad Hossein Salari Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Amir Hassan Zarnani Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran AND Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran Mohammad Reza Pourmand Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Hojat Ahmadi Department of Bacterial Vaccines and Antigen Production, Pasteur Institute of Iran, Tehran, Iran Mohamad Hassan Shirazi Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Mahmood Jeddi-Tehrani Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran 2015 10 16 No Abctract https://ijaai.tums.ac.ir/index.php/ijaai/article/view/513 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/513/520