Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 13 1 2014 02 15 1 10 EN Azad Saei Department of Immunology, Tehran University of Medical Sciences, Tehran, Iran Roobina Boghozian Department of Immunology, Tehran University of Medical Sciences, Tehran, Iran Reza Mirzaei Department of Immunology, Tehran University of Medical Sciences, Tehran, Iran Arezoo Jamali Department of Laboratory Sciences, School of Paramedicine, University of Medical Sciences, Tehran, Iran Behrooz Vaziri Protein Chemistry Unit, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran Jamshid Hadjati Department of Immunology, Tehran University of Medical Sciences, Tehran, Iran 2015 10 16 Fully mature dendritic cells (DCs) play pivotal role in inducing immune responses and converting naïve T lymphocytes into functional Th1 cells. We aimed to evaluate Listeria Monocytogenes-derived protein fractions to induce DC maturation and stimulating T helper (Th)1 immune responses. In the present study, we fractionated Listeria Monocytogenes-derived proteins by adding of ammonium sulfate in a stepwise manner. DCs were also generated from C57BL/6 mice bone marrow precursor cells. Then, the effects of protein fractions on bone marrow derived DC (BMDC) maturation were evaluated. In addition, we assessed the capacity of activated DCs to induce cytokine production and proliferation of lymphocytes. Listeria-derived protein fractions induced fully mature DCs expressing high costimulatory molecules such as CD80, CD86 and CD40. DCs that were activated by selected F3 fraction had low capacity to uptake exogenous antigens while secreted high levels of Interleukine (IL)-12. Moreover, lymphocytes cultured with activated BMDCs produced high amounts of IFN-γ  and  showed  higher  proliferation  than  control.  Listeria derived protein  fractions differently influenced DC maturation. In conclusion, Listeria protein activated-BMDCs can be used as a cell based vaccine to induce anti-tumor immune responses. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/483 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/483/354

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 13 1 2014 02 15 11 18 EN Guqin Zhang Department of Respiratory Medicine, Zhongnan Hospital of Wuhan University, China Jiong Yang Department of Respiratory Medicine, Zhongnan Hospital of Wuhan University, China Ping Li Department of Respiratory Medicine, Zhongnan Hospital of Wuhan University, China Jie Cao Department of Respiratory Medicine, Zhongnan Hospital of Wuhan University, China Hanxiang Nie Department of Respiratory Medicine, Renmin Hospital of Wuhan University, China 2015 10 16 Mast cells play an important role in a variety of inflammatory diseases, particularly asthma and atopy. Peroxisome proliferator-activated receptor gamma (PPARγ) is a member of the large nuclear hormone  receptor  transcription  factor  superfamily, and  has  been  recently implicated in the anti-inflammatory response. To investigate a possible role for PPARγ in human mast cells, we studied the effects of a PPARγ  ligand,  rosiglitazone  (RG),  on  stem  cell  factor  (SCF)-induced  migration  and fibronectin-induced adhesion in human mast cell-1(HMC-1) cells. It was found  that HMC-1 cells expressed PPARγ mRNA. RG inhibited SCF-induced HMC-1 cell migration and fibronectin-induced HMC-1 cell adhesion, the selective PPARγ antagonist GW9662 prevented the inhibitory effect of RG on HMC-1 cells. In conclusion, RG inhibits the migration and adhesion of HMC-1 cells by a PPARγ-dependent mechanism. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/482 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/482/355

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 13 1 2014 02 15 19 25 EN Somayeh Ahmadlou Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Mohsen Akhiani Department of Rheumatology, Alborz Hospital, Karaj, Iran Ahmad Salimzadeh Rheumatology Research Center, Sina Hospital, Tehran University of Medical Sciences, Tehran, Iran Mohammad Keramatipour Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran 2015 10 16 The association of rs10818488 SNP located in TRAF1/C5 region with Rheumatoid Arthritis (RA), has been picked up by genome-wide association studies. Independent studies in different populations revealed inconsistent results. The aim of this study was to investigate the possible association of this SNP with RA in Iranian population. A total of 362 cases and 422 healthy controls were recruited in this study. Genomic DNA was extracted from whole blood and the genotyping was performed by PCR-RFLP (Polymerase Chain Reaction– estriction Fragment Length Polymorphism). A set of genotypes was confirmed by sequencing. Genotype and allele frequencies were compared between the case and control groups. Analysis indicated a higher frequency of A allele in cases, although the difference was not statistically significant (Chi-square=2.8, p=0.09). Comparison of genotype frequencies, revealed higher frequencies of AA and AG genotypes in case group but statistically the difference was not significant (Chi-square=2.72, p=0.25).  These findings suggest that the rs10818488 in TRAF1/C5 region is not associated with rheumatoid arthritis in Iranian population. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/481 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/481/356

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 13 1 2014 02 15 26 32 EN Cui-cui Duan Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China Ai-li Li Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China Li-jie Yang Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China Rui Zhao Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China Wen-guang Fan Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China Gui-cheng Huo Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China 2015 10 16 Cow’s milk allergy is one of the most common food allergies in childhood. Beta- lactoglobulin (β-  Lg) is  a dominant  allergen in  cow’s milk. Hydrolysis is  known as  an effective method to reduce the allergenicity of proteins. Thus, the objective of this study was to compare the allergenicity of β-Lg and its hydrolysates using an animal model. Twenty four BALB/c mice were divided into three groups and subcutaneously injected with native bovine β-Lg and its hydrolysates on days 0, 7 and 14. During the sensitization period, a number of systemic anaphylactic indicators were observed in mice sensitized by β- Lg compared to those sensitized by hydrolysates of β-Lg. Mice sensitized by hydrolysates of β-Lg showed a significantly lower spleen lymphocyte proliferation level than that sensitized by intact β-Lg. Antibody levels of β-Lg-specific IgE in serum induced by native β-Lg were significantly high. Plasma histamine levels were also evaluated  and  showed  the  same  trend  as  IgE.  Moreover,  the  hydrolysates  of  β-Lg significantly down-regulated IL-4 and IL-5 secretions in serum. These results suggested that enzymatic hydrolysis could reduce the allergenicity of β-Lg. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/480 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/480/357

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 13 1 2014 02 15 33 39 EN Leila Mohammadi Amirabad Department of Medical Genetics, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran Ghasem Ahangari Department of Medical Genetics, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran Gholamreza Derakhshan Deilami Department of lung diseases, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran 2015 10 16 Asthma is a chronic inflammatory disorder of the airways. The stress is a factor for asthma which indicates a disorder in the function of communicational mediators of nervous and  immunological systems  such  as  neurotransmitters.  A  study  indicated  that  blood serotonin concentration increases in asthmatic patients. Other study indicates that one kind of the serotonin  receptors, named 5HT3A, on PBMCs causes secretion of series of pro- inflammatory cytokines which play important  roles in allergic asthma disease. Thus, we evaluated the ratio expression level of 5HT3A subtype receptors in asthma. The Peripheral Blood Mononuclear Cells were separated from whole blood of 30 allergic asthmatic patients and 30 normal controls by a gradient density centrifugation technique, then the total cellular RNA was extracted and the cDNA was synthesized. This process was followed by real-time PCR using primer pairs specific for 5-hydroxytryptamine 3A subtype receptor mRNA and beta-actin as internal control. Results  revealed  that  relative  gene  expression  of  5-hydroxytryptamine 3A  subtype receptor  increased significantly in Peripheral Blood Mononuclear  Cells of  patients  with asthma in comparison with normal individuals. To conclude, considering 5-hydroxytryptamine 3A subtype receptor role in accomplishment of asthma symptoms, this increase in its expression may exacerbate the seriousness of asthma disease. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/479 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/479/358

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 13 1 2014 02 15 40 46 EN Mitra Samareh Fekri Department of Pulmonology and Physiology, Kerman University of Medical Sciences, Kerman, Iran Hamid Reza Poursalehi Animal Physiology, Physiology Research Center, Kerman University of Medical Sciences, Kerman, Iran Hamid Najafipour Animal Physiology, Physiology Research Center, Kerman University of Medical Sciences, Kerman, Iran Beydolah Shahouzahi Animal Physiology, Physiology Research Center, Kerman University of Medical Sciences, Kerman, Iran Nasrin Bazargan Harandi Department of Pediatric Asthma and Allergy and Immunology; Kerman University of Medical Sciences, Kerman, Iran 2015 10 16 Gastroesophageal reflux disease (GERD)  is defined with clinical symptoms  of  heart burning and regurgitation. It may be associated with external esophageal symptoms such as chronic cough, asthma, laryngitis, chronic lung disease, sinusitis and pulmonary fibrosis. In the present study, rats with chronic aspiration of gastroduodenal contentswere studied for  cellular phenotypes  and  cytokine concentrations  in bronchoalveolar lavage and  lung tissue. Thirty-six male  Albino  N-MRI  rats  were  randomly  divided  into  six  groups.  After anesthesia and tracheal intubation, the animals received either 0.5ml/kg of normal saline (control), gastric juice, pepsin, hydrochloric acid or bile salts by injection into their lungs twice a  week  for  8  weeks. In  sham  group  nothing  was  injected.  Thereafter,  cellular phenotypes  and  cytokine  concentrations  of  Interleukine  (IL)-1α, IL-1β,  Transforming Growth  Factor  (TGF)-β,  Tumor  Necrosis  Factor  (TNF)-α,  and  IL-6  were assessed in bronchoalveolar lavage and lung tissue homogenates.  The numbers of epithelial cells, macrophages, neutrophils and lymphocytes in BAL and levels of cytokines IL-1α, IL-6, TNF-α and TGF-β in BAL and lung tissue of test groups were significantly higher  than  the  control  group.  Aspiration  of  bile salts caused more cytokine levels and inflammatory cells compared to other reflux components. It can be concluded that GERD with increased cytokines and inflammatory cells in lung could cause or exacerbate asthma and pulmonary fibrosis. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/478 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/478/359

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 13 1 2014 02 15 47 54 EN Ayşe Yılmaz Department of Chest Diseases, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey Birgül E Cumurcu Department of Psychiatry, Inonu University, Turgut Ozal Medical Center, Malatya, Turkey Ilker Etikan Department of Bioistatistics and Medical Informatics, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey Ekrem Hasbek Department of Psychiatry, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey Sibel Doruk Department of Chest Diseases, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey 2015 10 16 Assesment of health related quality of life (HRQL) is increasingly recognized as an important part of the patient care in asthma. We aimed to evaluate the effect of personality disorders (PDs) on HRQL and the severity of disease in asthmatic patients. Ninety seven consecutive patients with asthma and ninety healthy controls were included. All  patients  completed  a  Short  Form-36  health  survey  (SF-36)  and  Structured  Clinical Interview Form for DSM-III-R Axis-II Disorders (SCID-II). All  SF-36  questionnaire  scores  of  asthmatic  patients  were  significantly  lower  than the   controls.   There   were   statistical   differences   between   the   asthma   severity   and the  mean  scores  of  vitality  and  emotional  role  difficulties  of  HRQL’s  subdomains (p=0.03, p=0.014, respectively). There was a weak but statistically significant correlation between the scores of pain and emotional role difficulties, and forced expiratory volume in 1 second (FEV1) (r=0.27, p=0.007; r=0.24, p=0.01, respectively). When compared to healthy  controls,  patients  with  asthma  had  higher  prevalence  of   PDs   (p=0.0001). The  most  common  PDs  found  in  asthmatic  patients  were  obsessive-compulsive  and avoidant   PDs.   HRQL   values   of   asthma   patients   with   PDs   were   significantly lower  than  patients  without  PDs  except  for  the  physical  functioning  subscores  on HRQL. We  conclude  that  patients  with  asthma  have  higher  prevalence  of  PDs,  which  is associated with their poor quality of life. A psychiatric evaluation may be beneficial in patients with asthma if a concomitant PD is suspected. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/477 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/477/360

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 13 1 2014 02 15 55 60 EN Candy Sodhi Department of Physiology, Christian Medical College and Hospital, Ludhiana, Punjab, India. Sheena Singh Department of Physiology, Christian Medical College and Hospital, Ludhiana, Punjab, India. Amit Bery Department of Medicine, Dayanand Medical College and Hospital, Ludhiana, Punjab, India 2015 10 16 Yoga which is used as an adjunct treatment for bronchial asthma is gaining popularity throughout the world. The objective of this study was to assess the effect of yoga on quality of life in patients with bronchial asthma. 120 non-smoking male and female patients of asthma in the age group of 17-50 years were randomized into two groups i.e. Group A (Yoga group) and Group B (control group). All patients remained on their prescribed medication, but Group A patients practiced yoga breathing exercises for 8 weeks. Asthma Quality of Life Questionnaire (AQLQ) and diary record was used to assess quality of life, number and severity of asthmatic attacks, and the dosage of the medication required at baseline and after 8 weeks. Group A subjects showed a statistically significant improvement in "symptoms", "activities" and "environmental" domains of AQLQ at 8 weeks (p<0.01) and significant reduction in daily number and severity of attacks, and the dosage of medication required at 4 and 8 weeks (p<0.01) compared to the baseline. Yoga breathing exercises used adjunctively with standard pharmacological treatment significantly improved quality of life in patients with bronchial asthma. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/476 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/476/361

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 13 1 2014 02 15 61 65 EN Behnaz Esmaeili Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Mohsen Ghadami Endocrinology and Metabolism Research Institute (EMRI), Tehran University of Medical Sciences, Tehran, Iran Mohammad Reza Fazlollahi Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Shirin Niroomanesh Department of Prenatalogy, Zanan Hospital, Tehran University of Medical Sciences, Tehran, Iran Lida Atarod Department of Pediatrics, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran Zahra Chavoshzadeh Pediatric Infection Research Center, Mofid Hospital, Shaheed Beheshti University of Medical Sciences, Tehran, Iran Zeinab Moradi Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Zahra Alizadeh Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Zahra Pourpak Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran AND Department of Allergy and Clinical Immunology, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran 2015 10 16 Leukocyte adhesion deficiency type-1(LAD-1) is one of the autosomal recessive immunodeficiency diseases that results from mutation in integrin beta 2 (ITGB2) gene. The aim of this study was to investigate molecular prenatal diagnosis of LAD-1. Four pregnant women with five fetuses (one twin fetus) with clinical and laboratory diagnosis of LAD-1 in their previous children were studied. The chorionic villus sampling (CVS) was obtained when mothers were in 10-12th weeks of gestation. Mutation analysis of ITGB2 gene for affected children revealed 3 misssense mutations (c.382G>A,   a   novel   mutation,   c.2146G>C,   and   c.715G>A)   and   one   splice  site novel mutation (c.1877+2T>C). All parents were heterozygous for these mutations. Consideration of affected gene regions for five CVS samples showed two homozygotes and one heterozygote for mutant allele and two homozygotes for normal allele. Interestingly, one  of  the  twin  fetuses  was  affected  and  another  was  normal.  Briefly, two  cases  of CVS samples were affected and three cases of remained CVS samples were unaffected. This is the first report of prenatal diagnosis of LAD-1 from Iran with two new mutations that can be used for genetic and prenatal diagnosis for all patients suspected to LAD1 and can be helpful to prevent the birth of affected children with LAD-1. This  abstract  was presented  in  the  Second  International  Congress  of  Immunology, Asthma and Allergy, Tehran, Iran 2013. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/475 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/475/362

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 13 1 2014 02 15 66 70 EN Ilad Alavi Darazam National Research Institute of Tuberculosis and Lung Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran Hossein Akhavan Zanjani Department of Internal Medicine, Shohadaye Tajrish Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran Davood Sanaee National Research Institute of Tuberculosis and Lung Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran Payam Tabarsi National Research Institute of Tuberculosis and Lung Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran Mostafa Alavi Moghaddam National Research Institute of Tuberculosis and Lung Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran Davood Mansouri National Research Institute of Tuberculosis and Lung Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran 2015 10 16 Chronic granulomatous disease is an inherited defect in intracellular killing of ingested microorganisms characterized by recurrent life threatening bacterial and fungal infections including invasive aspergillosis in early childhood. We report  a  disseminated aspergillosis as  the  representative  of  adult  onset  chronic granulomatous disease without previous infection, with dramatic response to combination of antifungal and interferon therapy. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/474 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/474/363

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 13 1 2014 02 15 71 84 EN Mahmoud Abbasi Department of Medical Ethics, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Ehsan Shamsi Gooshki Department of Medical Ethics, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Neda Allahbedashti Department of Medical Ethics, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran 2015 10 16 Abortion traditionally means miscarriage and is still known as a problem, which societies have been trying to reduce its rate by using legal means. Despite the fact that  pregnant women and fetuses have been historically supported;  abortion  was firstly criminalized in 1926 in Iran, 20 years after establishment of modern legal system. During next 53 years this situation changed dramatically, thus in 1979, the time of Islamic Revolution, aborting fetuses up to 12 weeks of conception and therapeutic abortion (TA) during the  entire period  of  pregnancy were legitimated, based on  regulations that  used medical justifications. After  1979 the  situation  changed  into  a  totally conservative and restrictive approach and new Islamic concepts as “Blood Money” and “Ensoulment” entered the legal debates around abortion. During next 33 years, again a trend of decriminalization for the act of abortion has been continued. Reduction of punishments and omitting retaliation for criminal abortions, recognizing fetal and maternal medical indications including some immunologic problems as legitimate reasons for aborting fetuses before 4 months of gestation and omitting the fathers’ consent as  a  necessary condition  for  TA  are  among  these  changes.  The  start  point  for  this decriminalization process  was  public  and  professional  need,  which  was  responded  by religious government, firstly by issuing juristic rulings (Fatwas) as a non-official way, followed by ratification of “Therapeutic Abortion Act” (TAA) and other  regulations as an official pathway. Here,   we   have   reviewed  this   trend   of   decriminalization,  the   role   of   public and  professional request  in initiating such  process  and  the  rule-based language of  Iran TAA. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/473 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/473/364