Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 13 2 2014 04 15 85 92 EN Nargess Arandi Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Abbas Mirshafiey Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Mahmood Jeddi-Tehrani Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran Mohammadreza Shaghaghi Research Center for Immunodeficiency, Pediatrics Center of Excellence, Children’s Medical Center, Tehran University of Medical Sciences, Tehran, Iran Bamdad Sadeghi Research Center for Immunodeficiency, Pediatrics Center of Excellence, Children’s Medical Center, Tehran University of Medical Sciences, Tehran, Iran Hassan Abolhassani Research Center for Immunodeficiency, Pediatrics Center of Excellence, Children’s Medical Center, Tehran University of Medical Sciences, Tehran, Iran Ramazan Ali Sharifian Clinic of Hematology and Oncology, Vali-Asr Hospital, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran Mohammad Saeid Rahiminejad Division of Pediatric Hematology Oncology, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran Asghar Aghamohammadi Research Center for Immunodeficiency, Pediatrics Center of Excellence, Children’s Medical Center, Tehran University of Medical Sciences, Tehran, Iran 2015 10 16 Immune thrombocytopenic  purpura (ITP) is an autoimmune bleeding disorder characterized by production  of auto-antibodies against platelet antigens. It is obvious that regulatory T cells (Tregs) have a major role in controlling immune homeostasis and preventing autoimmunity. To investigate the frequency and functions of Tregs, twenty ITP patients and twenty age- and sex- matched healthy controls were recruited. The peripheral blood mononuclear cells were isolated and the proportion of Tregs was defined by flow cytometry method. The expression of immune-regulatory markers, cytotoxic T-lymphocyte associated antigen-4 (CTLA-4) and glucocorticoid induced tumor necrosis  factor  receptor  (GITR)  were  also  assessed by  quantitative  Real-time polymerase chain reaction TaqMan method. For evaluation of Treg function, Tregs were enriched and their ability to inhibit proliferation of T cells was measured and levels of immune-regulatory cytokines IL-10 and Transforming growth factor beta (TGF-β) were also measured.Results showed that the frequency of Tregs  and  the  mean  fluorescence  intensity  of  forkhead  box  P3  (FOXP3)  protein  significantly decreased in ITP patients compared to those in healthy controls. In addition, there was a significant reduction  in relative expression of both  CTLA-4 and GITR  mRNA  in ITP  patients (p=0.02 and p=0.006, respectively). The suppressive function of Tregs also diminished in ITP patients compared to controls. Both  IL-10 and TGF-β  cytokines were produced  in lower amounts  in ITP  patients than controls. It  could  be  concluded  that  alteration  in  Treg  frequency and  functional  characteristics might  be responsible for loss of self-tolerance and subsequently destructive immune responses observed in ITP patients. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/472 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/472/365

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 13 2 2014 04 15 93 97 EN Hui Zhong Department of Pediatrics, Shanghai First People's Hospital, Shanghai Jiaotong University, Shanghai, China Xiao Jian Zhou Department of Pediatrics, Shanghai First People's Hospital, Shanghai Jiaotong University, Shanghai, China Jian Gou Hong Department of Pediatrics, Shanghai First People's Hospital, Shanghai Jiaotong University, Shanghai, China 2015 10 16 Cytokine production  in response to allergens may influence the development of atopy- predisposing immune responses, initializing the  early programming of  allergy and  asthma. Vitamin D  intake  may be  protective  due  to  its  immunoregulatory properties,  that  may contribute  to  influence the expression of  the atopic phenotype  initiated in early life. The objective of our study was to investigate the effects of 1,25-(OH)2D3 on allergen-stimulated expression of asthma related cytokines in cord blood T cells. Cord blood samples were collected from the umblilical vein of 24 term deliveries during labor, CD4+T cells derived from cord blood mononuclear cells (CBMCs), were cultured for 72 hours with ovalbumin (OVA), β-lactoglobulin (β-LG), respectively, in presence or absence of 1,25-(OH)2D3  to detect the levels of interleukin-13 (IL-13) and interleukin-17 (IL-17) in culture supernatants and the mRNA expressions in CD4+T cells. After  allergens  stimulation,  CD4+T   cells  showed  an  increase  of  IL-13  and  IL-17 production, while cultured in the presence of 1,25-(OH)2D3  displayed a statistically significant down-regulation of allergen-induced expression of IL-13 and IL-17 in CD4+T cells. These  results indicated that  allergens may induce changes in CD4+T  cell function  to increase inflammatory cytokine production. 1,25-(OH)2D3  modulated the capacity of CD4+T cells in response to allergens, which might be protective for allergy. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/471 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/471/366

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 13 2 2014 04 15 98 103 EN Javad Ghaffari Department of Allergy and Clinical Immunology, Molecular and Cellular Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Reza Farid Hossiani Department of Allergy and Clinical Immunology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Alireza Khalilian Department of Biostatistics, Faculty of Health, Mazandaran University of Medical Sciences, Sari, Iran Negin Nahanmoghadam Department of General Pediatrics, Mazandaran University of Medical Sciences, Sari, Iran Ebrahim Salehifar Department of Pharmacology, Mazandaran University of Medical Sciences, Sari, Iran Houshang Rafatpanah Department of Immunology, Mashhad University of Medical Sciences, Mashhad, Iran 2015 10 16 Asthma is the most  common  chronic inflammatory disorder characterized by cough, wheezing and dyspnea in children. Nutrition  is an important  factor which influences on induction and exacerbation of asthma. There are controversies to use Vitamin E in asthmatic patients. The aim of this study was to evaluate the effect of vitamin E supplement in children with moderate asthma. This is a randomized double blind placebo-controlled trial performed on children (age 2-17 years old) with moderate asthma (5-17 years old) from March 2010 to March 2012. Case group were treated with fluticasone and vitamin E (50mg/day) and control group received fluticasone plus placebo for 8 weeks. Out of 300 cases, 240 cases completed the study. Female to male ratio was 0.84. Serum level of Vitamin E significantly increased after treatment in intervention group. FEV1 and FEV1/FVC ratio was ignificantly improved in case group compared to the control group. It can be concluded that vitamin E supplement could improve clinical manifestations and pulmonary function test in children with moderate asthma. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/470 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/470/367

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 13 2 2014 04 15 104 109 EN Katayoon Bidad Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Hassan Heidarnazhad Tobacco Prevention and Control Research Center, National Research Institute of Tuberculosis and Lung Disease, Shahid Beheshti University of Medical Sciences, Tehran, Iran Zahra Pourpak Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Giti Shayestefar Department of Obstetrics, Arash Hospital, Tehran University of Medical Sciences, Tehran, Iran Heshmat Moayeri Department of Endocrinology, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran Nasrin Zendehdel Department of Internal Medicine, Shohada Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran 2015 10 16 Asthma and  gastroesophageal reflux disease (GERD)  are two  common  problems  in pregnancy and they affect pregnancy in several ways. In this study, we aimed to evaluate GERD and asthma in pregnant women who referred for prenatal care visits. One-hundred  and seventy three  pregnant  women with a complaint of  dyspnea were included in the study. A questionnaire was filled and lung function tests were performed. All patients  were visited by a respiratory specialist and  questionnaires were evaluated by a gastroenterologist. Out  of the total number of women studied, 37% were diagnosed to have asthma and 36.4% were non-asthmatics. Twenty six percent of the pregnant women who had symptoms and signs of asthma with normal spirometry were classified as probable to have asthma. GERD was diagnosed in 80.9% of the pregnant women, but it was not significantly higher in asthmatic or probable asthmatic women compared to non-asthmatic ones. However, severity of GERD was significantly higher in asthmatic pregnant women compared to the others. In conclusion, the prevalence of GERD  was quite high in pregnant women, irrespective of the fact that they were asthmatic or non-asthmatic. Further studies evaluating women throughout pregnancy will inform us more about this relationship. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/469 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/469/368

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 13 2 2014 04 15 110 119 EN Zoleikha Moazezi Department of Endocrinology, Ayatollah Rohani Hospital, Babol University of Medical Sciences, Babol, Iran. Azam Hosseinian Department of Endocrinology, Ayatollah Rohani Hospital, Babol University of Medical Sciences, Babol, Iran. Ensiyeh Ahmad Moazam Department of Endocrinology, Ayatollah Rohani Hospital, Babol University of Medical Sciences, Babol, Iran. Mohamed Bagher Eslami Department of Pathobiology, Faculty of Health, Tehran University of Medical Sciences, Tehran, Iran. Ezatollah Mosavi Department of Microbiology and Immunology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran. Haleh Akhavan-Niaki Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran. Ali Bijani Non- Communicable Pediatrics Diseases Research Center, Babol University of Medical Sciences, Babol, Iran. Nanette Schloot Department of Immunobiology,  German Diabetes Research Center, Dusseldorf, Germany. Amrollah Mostafazadeh Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran. 2015 10 16 It has been widely thought that diabetic patients are prone to infections due to hyperglycemia induced immunodeficiency; the present study was designed to examine this opinion. In diabetic patients and normal control groups T-cell reactivity to hsp-60 molecule, tetanus toxoid recall antigen (TT) and phytohemagglutinin-A (PHA) mitogen were evaluated The number of circulating IFN-γ, IL-10 and IL-13 cytokine producing cells stimulated with above antigens or mitogen as well as the serum levels of Th1/Th2 type cytokines were determined. Total serum immunoglobulins (IgG, IgA, IgM), C3, C4 and CH50 were also measured. Diabetic patients showed a positive circulating T-cell reactivity to human recombinant hsp60 However, this reactivity was significantly lower in comparison to control group (p<0.001). All other examined factors were not significantly different between diabetic and normal subjects except for the number of IFN-γ and IL-13 producing cells in response to PHA stimulation, which was higher in control gtroup (p=0.006, 0.018, respectively). The mean serum concentration of IgA in diabetic patients was 245.86 ± 115.05 mg/dl versus 192.96 ± 105.33 mg/dl in healthy control group (p<0.018). We were not able to demonstrate any substantial mitigation in cellular arms of immune reaction to some prominent T-cell antigens and mitogens, as well as, in main parameters of humoral immunity of diabetic patients, thus, the common notion of believing that patients with diabetes suffering from immunodeficiency should be revised. It is much more appropriate that "altered immunity" is applied instead of "immunodeficiency" to explain the immunity condition in this group of patients. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/468 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/468/369

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 13 2 2014 04 15 120 124 EN Sara Zahedifard Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran. Elahe Rashidi Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran. Sedigheh Shams Department of Pathology, Children Hospital Medical Center, Tehran University of Medical Sciences, Tehran, Iran. Shiva Saghafi Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran. Mohammadreza Fazlollahi Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran. Azadeh Talebzadeh Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran. Anoushirvan Kazemnejad Department of Bio; a  Short  Form-36  health  survey  (SF-36)  and  Structured  Clinical Interview Form for DSM-III-R Axis-II Disorders (SCID-II). All  SF-36  questionnaire  scores  of  asthmatic  patients  were  significantly  lower  than the   controls.   There   were   statistical   differences   between   the   asthma   severity   and the  mean  scores  of  vitality  and  emotional  role  difficulties  of  HRQL’s  subdomains (p=0.03, p=0.014, respectively). There was a weak but statistically significant correlation between the scores of pain and emotional role difficulties, and forced expiratory volume in 1 second (FEV1) (r=0.27, p=0.007; r=0.24, p=0.01, respectively). When compared to healthy  controls,  patients  with  asthma  had  higher  prevalence  of   PDs   (p=0.0001). The  most  common  PDs  found  in  asthmatic  patients  were  obsessive-compulsive  and avoidant   PDs.   HRQL   values   of   asthma   patients   with   PDs   were   significantly lower  than  patients  without  PDs  except  for  the  physical  functioning  subscores  on HRQL. We  conclude  that  patients  with  asthma  have  higher  prevalence  of  PDs,  which  is associated with their poor quality of life. A psychiatric evaluation may be beneficial in patients with asthma if a concomitant PD is suspected. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/477 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/477/360

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 13 1 2014 02 15 55 60 EN Candy Sodhi Department of Physiology, Christian Medical College and Hospital, Ludhiana, Punjab, India. Sheena Singh Department of Physiology, Christian Medical College and Hospital, Ludhiana, Punjab, India. Amit Bery Department of Medicine, Dayanand Medical College and Hospital, Ludhiana, Punjab, India 2015 10 16 Yoga which is used as an adjunct treatment for bronchial asthma is gaining popularity throughout the world. The objective of this study was to assess the effect of yoga on quality of life in patients with bronchial asthma. 120 non-smoking male and female patients of asthma in the age group of 17-50 years were randomized into two groups i.e. Group A (Yoga group) and Group B (control group). All patients remained on their prescribed medication, but Group A patients practiced yoga breathing exercises for 8 weeks. Asthma Quality of Life Questionnaire (AQLQ) and diary record was used to assess quality of life, number and severity of asthmatic attacks, and the dosage of the medication required at baseline and after 8 weeks. Group A subjects showed a statistically significant improvement in "symptoms", "activities" and "environmental" domains of AQLQ at 8 weeks (p<0.01) and significant reduction in daily number and severity of attacks, and the dosage of medication required at 4 and 8 weeks (p<0.01) compared to the baseline. Yoga breathing exercises used adjunctively with standard pharmacological treatment significantly improved quality of life in patients with bronchial asthma. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/476 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/476/361

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 13 1 2014 02 15 61 65 EN Behnaz Esmaeili Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Mohsen Ghadami Endocrinology and Metabolism Research Institute (EMRI), Tehran University of Medical Sciences, Tehran, Iran Mohammad Reza Fazlollahi Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Shirin Niroomanesh Department of Prenatalogy, Zanan Hospital, Tehran University of Medical Sciences, Tehran, Iran Lida Atarod Department of Pediatrics, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran Zahra Chavoshzadeh Pediatric Infection Research Center, Mofid Hospital, Shaheed Beheshti University of Medical Sciences, Tehran, Iran Zeinab Moradi Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Zahra Alizadeh Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Zahra Pourpak Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran AND Department of Allergy and Clinical Immunology, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran 2015 10 16 Leukocyte adhesion deficiency type-1(LAD-1) is one of the autosomal recessive immunodeficiency diseases that results from mutation in integrin beta 2 (ITGB2) gene. The aim of this study was to investigate molecular prenatal diagnosis of LAD-1. Four pregnant women with five fetuses (one twin fetus) with clinical and laboratory diagnosis of LAD-1 in their previous children were studied. The chorionic villus sampling (CVS) was obtained when mothers were in 10-12th weeks of gestation. Mutation analysis of ITGB2 gene for affected children revealed 3 misssense mutations (c.382G>A,   a   novel   mutation,   c.2146G>C,   and   c.715G>A)   and   one   splice  site novel mutation (c.1877+2T>C). All parents were heterozygous for these mutations. Consideration of affected gene regions for five CVS samples showed two homozygotes and one heterozygote for mutant allele and two homozygotes for normal allele. Interestingly, one  of  the  twin  fetuses  was  affected  and  another  was  normal.  Briefly, two  cases  of CVS samples were affected and three cases of remained CVS samples were unaffected. This is the first report of prenatal diagnosis of LAD-1 from Iran with two new mutations that can be used for genetic and prenatal diagnosis for all patients suspected to LAD1 and can be helpful to prevent the birth of affected children with LAD-1. This  abstract  was presented  in  the  Second  International  Congress  of  Immunology, Asthma and Allergy, Tehran, Iran 2013. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/475 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/475/362

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 13 1 2014 02 15 66 70 EN Ilad Alavi Darazam National Research Institute of Tuberculosis and Lung Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran Hossein Akhavan Zanjani Department of Internal Medicine, Shohadaye Tajrish Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran Davood Sanaee National Research Institute of Tuberculosis and Lung Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran Payam Tabarsi National Research Institute of Tuberculosis and Lung Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran Mostafa Alavi Moghaddam National Research Institute of Tuberculosis and Lung Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran Davood Mansouri National Research Institute of Tuberculosis and Lung Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran 2015 10 16 Chronic granulomatous disease is an inherited defect in intracellular killing of ingested microorganisms characterized by recurrent life threatening bacterial and fungal infections including invasive aspergillosis in early childhood. We report  a  disseminated aspergillosis as  the  representative  of  adult  onset  chronic granulomatous disease without previous infection, with dramatic response to combination of antifungal and interferon therapy. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/474 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/474/363

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 13 1 2014 02 15 71 84 EN Mahmoud Abbasi Department of Medical Ethics, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Ehsan Shamsi Gooshki Department of Medical Ethics, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Neda Allahbedashti Department of Medical Ethics, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran 2015 10 16 Abortion traditionally means miscarriage and is still known as a problem, which societies have been trying to reduce its rate by using legal means. Despite the fact that  pregnant women and fetuses have been historically supported;  abortion  was firstly criminalized in 1926 in Iran, 20 years after establishment of modern legal system. During next 53 years this situation changed dramatically, thus in 1979, the time of Islamic Revolution, aborting fetuses up to 12 weeks of conception and therapeutic abortion (TA) during the  entire period  of  pregnancy were legitimated, based on  regulations that  used medical justifications. After  1979 the  situation  changed  into  a  totally conservative and restrictive approach and new Islamic concepts as “Blood Money” and “Ensoulment” entered the legal debates around abortion. During next 33 yea