Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 13 3 2014 06 15 147 156 EN Yadollah Shakiba Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Susan Koopah Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Ahmad Reza Jamshidi Rheumatology Research Centre, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran. Ali Akbar Amirzargar Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Ahmad Massoud Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Amir Kiani Molecular Diagnostic Research Center, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran. Mohammad Hossein Nicknam Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Bahareh Nazari Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Behrouz Nikbin Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran AND Molecular Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran. 2015 10 16 In  this  study  we  determined  the  frequency,  sensitivity  and  specificity  of  anti  cyclic citrullinated peptides (anti-CCP) IgG antibody, total rheumatoid factor (RF-T), and RF isotypes in Iranian patients with rheumatoid arthritis (RA) and their association with age, clinical and serological parameters. Anti-CCP and RF-T and RF isotypes level were measured in 418 patients and 399 healthy controls by enzyme-linked immunosurbant assay (ELISA). Additionally, serum C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), visual analog scale (VAS) and disease activity score (DAS28) were evaluated in RA patients. The anti-CCP was positive in 53.1% of RA patients and 4.7% of controls. The frequency of RF-T was 61.87% and 17.66% in RA patients and controls respectively. The prevalence of RF isotypes in RA patients was 46.52% for RF-IgM, 23.47% for RF-IgA and 21.74% for RF-IgG. 31.39% of RA patients were RF-IgM positive without RF-IgA and RF-IgG and 21.9% were positive for all three RF classes. The anti-CCP positive patients showed increased number of swollen joints. On the other hand, RF-T positive patients exhibited a longer disease duration, lower age of onset and also higher ESR, CRP level and increased swollen joints. RF-T titer was significantly higher in RA patients with active disease compared to remission, low and moderate active groups. The sensitivity and specificity were 53.1, 95.3 for anti-CCP antibody and 61.8, 82.3 for RF-T. Our results support that anti-CCP and RF titer maybe valuable in estimation of disease activity and other inflammatory parameters in RA patients. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/462 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/462/375

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 13 3 2014 06 15 157 165 EN Monireh Mohsenzadegan Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Fahimeh Fattahi Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran. Fatemeh Fattahi Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran AND Department of Pathology and Medical Biology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands. Abbas Mirshafiey Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Mohammad Reza Fazlollahi Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran. Fariba Naderi Beni Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Masoud Movahedi Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran AND Department of Immunology and Allergy, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran. Zahra Pourpak Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran AND Department of Immunology and Allergy, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran. 2015 10 16 Chronic  granulomatous  disease (CGD)  is a rare primary immunodeficiency disorder characterized by a greatly increased susceptibility to severe fungal and bacterial infections caused  by  defects  in  NADPH   oxidase  of  phagocytic  cells. We  aimed  to  investigate immunophenotype alterations of naïve and memory B cells and B1a cells in peripheral whole blood from Iranian patients with CGD. Flow cytometric analysis was performed  on  peripheral blood  samples from  31 CGD patients and 23 healthy controls (HC) to study naïve (IgD+/CD27-), memory (CD27+) B and B1a (CD5+) cells. Soluble CD27 (sCD27) and immunoglobulins were also measured by ELISA and the nephelometric method, respectively. We found significantly higher levels of naïve B cells and B1a cells but lower levels of memory B cells in CGD patients compared to HC. There was no significant difference in soluble CD27 (sCD27) alteration between CGD patients and HC. Our findings suggested a role for NADPH oxidase in process of B cell differentiation and impairing conversion of naïve B cells to  memory B cells and altered B1a cells in CGD patients.  Increased   susceptibility  of   CGD   patients   to   opportunistic   infections   and autoimmune disorders could be partly explained by the altered phenotype of B lymphocytes in these patients. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/461 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/461/376

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 13 3 2014 06 15 166 173 EN Marzieh Tavakol Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran Ali Kouhi Otorhinolaryngology Research Center, Amir Alam Hospital, Department of Otolaryngology, Tehran University of Medical Sciences, Tehran, Iran. Hassan Abolhassani Research Center for Immunodeficiencies, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran AND Division of Clinical Immunology, Department of Laboratory Medicine, Karolinska Institutet at the Karolinska University Hospital Huddinge, Stockholm, Sweden. Alireza Ghajar Research Center for Immunodeficiencies, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran. Mohsen Afarideh Research Center for Immunodeficiencies, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran. Shervin Shahinpour Research Center for Immunodeficiencies, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran. Asghar Aghamohammadi Research Center for Immunodeficiencies, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran. 2015 10 16 The  main  clinical presentation  of  patients  with  primary antibody  deficiency (PAD) incorporates upper respiratory tract infections comprising otitis media, sinusitis and pneumonia.  This  study  was  designed  to  investigate clinical and  paraclinical otological complications in major types of PAD.  A cross sectional study was conducted on 55 PAD patients with diagnosis of selective IgA deficiency, common variable immunodeficiency (CVID), X-linked agammaglobulinemia (XLA), and hyper IgM syndrome. All patients underwent otological examinations, audiometry, and auditory brain stem response.  Otological complications were detected in 54.5% of PAD patients. Conductive hearing loss was the main finding amongst PID patients (73.3%) followed by sensorineural hearing loss which was present in 8 cases. Otitis media with effusion (21.8%), chronic otitis media (27.2%), tympanosclerosis with intact tympanic membrane (5.4%) and auditory neuropathy (3.6%)  were  most   important   found complications.  CVID   and   XLA  patients   with prophylactic usage of antibiotics had lower rate of audiological complications (p=0.04) and otitis media with effusion (p=0.027). As our results showed, asymptomatic otological findings were not rare in PAD patients; therefore, a systematic otological investigation is recommended as an integral part of the management and follow-up of these patients. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/460 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/460/377

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 13 3 2014 06 15 174 183 EN Hossein Mortazavi Autoimmune Bullous Diseases Research Center, Tehran University of Medical Sciences, Tehran, Iran AND Department of Dermatology, Tehran University of Medical Sciences, Tehran, Iran. Nafiseh Esmaili Autoimmune Bullous Diseases Research Center, Tehran University of Medical Sciences, Tehran, Iran AND Department of Dermatology, Tehran University of Medical Sciences, Tehran, Iran. Somayeh Khezri Autoimmune Bullous Diseases Research Center, Tehran University of Medical Sciences, Tehran, Iran AND Department of Dermatology, Tehran University of Medical Sciences, Tehran, Iran. Ali Khamesipour Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, Iran. Iman Vasheghani Farahani Sharif University of Technology, Tehran, Iran. Maryam Daneshpazhooh Autoimmune Bullous Diseases Research Center, Tehran University of Medical Sciences, Tehran, Iran AND Department of Dermatology, Tehran University of Medical Sciences, Tehran, Iran. Maryamdanesh.pj@gmail.com. Nima Rezaei Research Center for Immunodeficiencies, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran AND Molecular Immunology Research Center; and Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. 2015 10 16 Pemphigus vulgaris is an autoimmune disease, in which the role of Th17 cytokines needs to be further explored. This study was performed to assess serum levels of three interleukins (IL) required for Th17 differentiation (IL-1β, IL-6 and IL-23) and two specific Th17 cytokines (IL-17 and IL-22) in a group of patients with pemphigus vulgaris, at baseline, 3 weeks and 6 months after of treatment. Correlations between anti-desmogleins and cytokines with disease severity as well as the influence of therapy on the above factors were assessed. Forty-three first-admitted pemphigus vulgaris patients with the active disease entered the study, but  only 31 completed  the  study. Forty-five healthy volunteers were recruited as a control group. The patients were treated with conventional immunosuppressive therapy (oral prednisolone and azathioprine). Cytokines and anti-desmogleins were measured, using enzyme- linked immunosorbent assay. General linear model was used to evaluate the changes over time. In patients at baseline, mean serum level of IL-6 was higher, while mean levels of IL-1β and IL-22 were lower than the controls. After 3 weeks of therapy, IL-1β and IL-6 levels showed a decreasing trend, whereas IL-22 showed an increasing trend. Mean anti-desmogleins 1 and 3 values  decreased  significantly during  the  time.  Anti-desmoglein  values  were  significantly correlated with disease severity. In conclusion, IL-1β and IL-6 could be involved in the pathogenesis of pemphigus vulgaris. The positive trend of IL-22 is a new finding and should be confirmed by further studies. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/459 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/459/378

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 13 3 2014 06 15 184 189 EN Hui Xu Department of Clinical Laboratory, Children's Hospital of Zhejiang University School of Medicine, Hangzhou, P.R. China. Wei Li Department of Clinical Laboratory, Children's Hospital of Zhejiang University School of Medicine, Hangzhou, P.R. China. Haidong Fu Department of Nephrology, Children's Hospital of Zhejiang University School of Medicine, Hangzhou, P.R. China. Guizheng Jiang Department of Clinical Laboratory, Children's Hospital of Zhejiang University School of Medicine, Hangzhou, P.R. China. 2015 10 16 The aim of this study was to investigate the possible influence of Interferon-gamma (IFN- γ) gene polymorphism +874 (A/T) (rs2430561) in the susceptibility and renal complications of patients with Henoch-Schonlein purpura (HSP). We also studied the effects of IFN-γ allelic variation on serum levels of pro-and anti-inflammatory cytokines in HSP patients. The  study  population  comprised  97  patients  suffering  from  HSP  and  97  control participants. Patients and controls  were genotyped for a single nucleotide polymorphism +874 (A/T) in the first intron of the IFN-γ gene by the TaqMan PCR method. Frequencies of individuals with IFN-γ  +874  AA, AT and TT genotypes were 77.3%,21.6% and 1% in HSP patients and 79.4%, 17.5% and 3.1% in controls, respectively. The frequency of the AA genotype in HSP patients with nephritis was slightly higher (83.3%) than in HSP patients without nephritis (73.8%). The allele A occurred more commonly in HSP patients with nephritis (92%) than in HSP patients without nephritis (86%), but these differences were not statistically significant (p= 0.469 and p= 0.244, respectively). In addition, significant difference in serum IL-10 levels between IFN-γ +874 different genotype groups was found. Our  results do not  support  a role for IFN-γ  gene polymorphism +874  (A/T) in the susceptibility to HSP and allelic variation at IFN-γ +874 locus had no effect on serum levels of cytokines in patients with HSP except for IL-10. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/458 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/458/379

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 13 3 2014 06 15 190 197 EN Nahid Eskandari Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. Reza Bastan Department of Immunopharmacology, Faculty of Medicine, Karaj University of Medical Sciences, Alborz, Iran. Maryam Ahmadi Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. Peter T Peachell Department of Immunology, Faculty of Medicine, University of Medical Sciences, Yorkshire, UK. 2015 10 16 Human  basophils play a key role in allergic diseases such as asthma and in a variety of  immunological disorders.  The  generation  of  IL-4  and  IL-13  can  be  induced  from basophil  by  IgE-mediated   and   non-IgE-mediated   mechanisms.  Time   and   stimulus- dependent  differences  in  the  regulation  of  these  cytokines  could  have  relevance  to their biological effects. The aim of the present study was activation of basophils in order to evaluate the extent of histamine, IL-4, and IL-13 generations. Basophil-enriched suspensions were prepared by Percoll gradients. The release of histamine and cytokines was assessed after activation with either anti-human IgE (1/1000 or1/10000, 4 h or 24 h) or IL-3 (100 ng/ ml, 24 h). Results were analysed statistically, using ANOVA test. Using  anti-IgE,  there  was  no  significant correlation  between  the  extent  of  either IL-4  (r=0.24,  p=0.35)  or  IL-13  (r=0.47,  p=0.098)  and  histamine  release. Using  IL-3 as stimulator, results showed that the extent of IL-13 correlated with histamine release(r=0.44,  p=0.036).  There  was  no  correlation  between  the  extent  of  IL-4  and the   degree   of   either   histamine   (r=0.077,   p=0.72)   or    IL-13   (r=0.162,   p=0.5). The  reproducibility of  cytokines isolated from  the  same donor  (on different occasions) indicated that the ability of anti-IgE to induce cytokines was consistently similar for a given donor. Our data showed that the pathways leading to IL-3-triggering histamine release and IL-13 generation show similarity. Donor-dependent  differences may be responsible for this wide range in the extent of releasibility. The ability of IL-3 to release cytokines from basophils showed a wider range. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/457 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/457/380

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 13 3 2014 06 15 198 206 EN Gholamreza Azizi Imam Hassan Mojtaba Hospital, Alborz University of Medical Sciences, Karaj, Iran. Mohsen Reza Haidari Department of Neurology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran. Mohammadreza Khorramizadeh Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Fatemeh Naddafi Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Reza Sadria Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Mohammad Hassan Javanbakht Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Reza Sedaghat Department of Anatomy and Pathology, Faculty of Medicine, Shahed University, Tehran, Iran. Farzaneh Tofighi Zavareh Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Abbas Mirshafiey Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. 2015 10 16 Experimental  autoimmune  encephalomyelitis (EAE)  is  a  mouse  model  for  multiple sclerosis (MS), This autoimmune disease is mainly mediated by adaptive and innate immune responses that lead to an inflammatory demyelination and axonal damage. Imatinib mesylate is a  selective protein  tyrosine kinase inhibitor  with immunomodulatory  properties  that abrogates multiple signal transduction pathways in immune cells. In the present research, our aim was to test the therapeutic efficacy of imatinib in experimental model of MS. We  performed  EAE  induction  in  23  female  C57  mice  by  myelin oligodendrocyte glycoprotein-35-55 (MOG35-55) in  Complete  Freund’s  Adjuvant (CFA) emulsion  and  used imatinib for treatment of EAE. The clinical evaluation and histopathology were assessed. Also for in vitro analysis, we used U-87 MG, C6 and WEHI-164 cell lines to evaluate the inhibitory effects of imatinib in cell proliferation, as well as pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) and matrix metalloproteinase (MMP) secretion. Our findings demonstrated that this drug had beneficial effects on EAE by attenuation in the severity and a delay in the onset of disease. In vitro, imatinib inhibited cell proliferation, MMP-2 expression and  activity and  also attenuated  the  production  of  proinflammatory cytokines. Imatinib with its potential therapeutic effects and immunomodulatory properties may be considered, after additional necessary tests and trials, for treatment of MS. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/456 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/456/381

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 13 3 2014 06 15 207 213 EN Zahra Kuroshli Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. Hamid Gourabi Department of Genetics at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. Masoud Bazrgar Department of Genetics at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. Mohammad Hossein Sanati Department of Medical Genetics, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran. Elmira Bahraminejad Department of Genetics at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. Khadije Anisi Department of Genetics at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. 2015 10 16 The human leukocyte antigen (HLA)-G molecule is expressed in cytotrophoblast cells, adult thymic epithelial cells, erythroblasts, pancreatic islets and mesenchymal stem  cells. Although, HLA-G expression in allotransplanted patients is correlated with a better allograft acceptance, it is associated with an advanced grade of the tumor in cancer. In addition to the role on the immune system, HLA-G is also involved in successful pregnancy through the embryo implantation, fetal survival and the initial steps of hematopoiesis and angiogenesis. The  aim of  this  study was determination  of  HLA-G  allele frequencies in a healthy population of Iran. In this research, we selected 100 samples from healthy Iranian individuals and henceforth, we used polymerase chain reaction (PCR) followed by sequencing technique for exon 2, 3, 4 and intron 2 of the gene for evaluating the HLA-G  alleles frequencies. Investigation of intronic (intron 2) variation is the  novelty of our study. The obtained results indicated thirteen alleles of HLA-G in Iranian individuals including G*01:01:01:01, G*01:06, G*01:01:01:06, G*01:01:02, G*01:01:03, G*01:01:05, G*01:01:06, G*01:01:07, G*01:01:08, G*01:03, G*01:04:01, G*01:04:03, and G*01:04:04. According to this study, the most prevalent alleles in the Iranian population were G*01:01:01:01 (52.5%), G*01:01:02  (16%)  and  G*01:04:03  (14.5%) and  also  the  lowest  alleles regarding  the frequency were G*01:01:01:06 (0.5%) and G*01:03 (0.5%). The results of G*01:01:01:01 and G*01:04:01 frequencies showed some similarities with the  polish population.  Our  results were similar to  the  north  Indian  population  for  the frequencies of G*01:06 and G*01:01:02. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/455 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/455/382

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 13 3 2014 06 15 214 217 EN Zohra Chadly Department of Pharmacology, University Hospital, Monastir, Tunisia. Karim Aouam Department of Pharmacology, University Hospital, Monastir, Tunisia. Amel Chaabane Department of Pharmacology, University Hospital, Monastir, Tunisia. Hichem Belhadjali Department of Dermatology, University Hospital, Monastir, Tunisia. Naceur Abderrazzak Boughattas Department of Pharmacology, University Hospital, Monastir, Tunisia. Jamel Eddine Zili Department of Dermatology, University Hospital, Monastir, Tunisia. 2015 10 16 A-10-year-old girl was referred to our department for multiple hyperpigmented plaques. One  week  previously,  she  had  been  given  one  suppository  of  acetylsalicylic acid  – phenobarbital for fever. Twelve hours after the drug intake the child developed pruritic red plaques on the left thigh. Six weeks after resolution of the acute reaction, patch tests were performed separately, with phenobarbital and acetylsalicylic acid. On 48-hour reading, only the phenobarbital patch test on residual pigmented lesion was positive. Because of possible cross-reactions between aromatic anticonvulsants, subsequent  patch  tests using carbamazepine and phenytoin on residual pigmented lesions were performed. They were all negative at 48-hour reading. To our knowledge, only two isolated pediatric cases of Phenobarbital-induced FDE have been reported in the literature. In this case report, as it was difficult to determine whether phenobarbital or acetylsalicylic acid was responsible for this reaction, subsequent patch tests allowed the identification of the culprit component since it was positive to phenobarbital. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/454 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/454/383

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 13 3 2014 06 15 218 219 EN Azar Baradaran Department of Pathology, Isfahan University of Medical Sciences, Isfahan, Iran. Hamid Nasri Department of Nephrology, Division of Nephropathology, Isfahan University of Medical Sciences, Isfahan, Iran. 2015 10 16  LETTER TO THE EDITOR https://ijaai.tums.ac.ir/index.php/ijaai/article/view/453 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/453/384