Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 8 2 2009 06 15 77 84 Abolhassani Mohsen Department of Immunology, Pasteur Institute of Iran, Tehran 13164, Iran Roux Kenneth H. Department of Biological Sciences, Florida State University, Tallahassee FL 32306, USA 2015 10 01 Tree nuts, including almond (prunus dulcis) are a source of food allergens often associated with life-threatening allergic reactions in susceptible individuals. Although the proteins in almonds have been biochemically characterized, relatively little has been reported regarding the identity of the allergens involved in almond sensitivity. The present study was undertaken to identify the allergens of the almond by cDNA library approach. cDNA library of almond seeds was constructed in Uni-Zap XR lamda vector and expressed in E. coli XL-1 blue. Plaques were immunoscreened with pooled sera of allergic patients. The cDNA clone reacting significantly with specific IgE antibodies was selected and subcloned and subsequently expressed in E. coli. The amino acids deducted from PCR product of clone showed homology to 60s acidic ribosomal protein of almond. The expressed protein was 11,450 Dalton without leader sequence. Immunoreactivity of the recombinant 60s ribosomal protein (r60sRP) was evaluated with dot blot analysis using pooled and individual sera of allergic patients. The data showed that r60sRP and almond extract (as positive control) possess the ability to bind the IgE antibodies. The results showed that expressed protein is an almond allergen. Whether this r60sRP represents a major allergen of almond needs to be further studied which requires a large number of sera from the almond atopic patients and also need to determine the IgE-reactive frequencies of each individual allergen. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/238 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/238/238

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 8 2 2009 06 15 85 93 Zarei Saeed 1 Monoclonal Antibody Research Center (MARC), Avicenna Research Institute (ARI), Iranian Academic Ce Jeddi-Tehrani Mahmood 2 Department of Immunochemistry, MARC, ARI Akhondi Mohammad Mehdi Reproductive Biotechnology Research Center (RBRC), ARI, Tehran, Iran Zeraati Hojjat 5 Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medica Pourheidari Foroughalsadat 6 East Health Center, Deputy of Health Affairs, Shahid Beheshti University of Medical Sciences, Tehr Ostadkarampour Mahyar 7 Nanobiotechnology Research Center (NBRC), ARI, Tehran, Iran Tavangar Banafsheh Monoclonal Antibody Research Center (MARC), Avicenna Research Institute (ARI), Iranian Academic Cent Shokri Fazel Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Iran 2015 10 01 Universal vaccination of neonates and children against diphtheria, tetanus and pertussis has had a tremendous impact on the control of these infectious diseases worldwide. Immunization by the triple diphtheria, tetanus and whole cell pertussis vaccine (DTwP) has been applied in Iran for almost 50 years. Periodic assessment of immunogenicity of this vaccine is an important aspect of successful mass vaccination programs. The present study was performed to assess the antibody response against tetanus, diphtheria and pertussis in a group of Iranian infants vaccinated with a local DTwP vaccine. In this prospective study, 330 infants received primary vaccination at 2, 4 and 6 months of age with DTwP vaccine manufactured by Razi Institute of Iran. Blood samples were taken 2-4 weeks after the third dose to assess seroprotection and geometric mean titers (GMT) of specific antibodies. Among the 283 infants who completed the vaccination course, 98.2% and 100% developed antibodies against diphtheria and tetanus, respectively. The GMT of antibodies to tetanus, diphtheria and pertussis, were 2.09 IU/ml, 2.08 IU/ml and 8.73 EU/ml, respectively. Comparison of the results obtained from this study with those from previous studies performed in other countries revealed a similar GMT and protection rates for diphtheria and tetanus components. In the absence of well-established serological criteria, judgment about protection rate against pertussis has not been possible. A prospective vaccination study using the local DTwP vaccine in parallel to a WHO approved standard vaccine, could enable assessment of immunogenicity of the pertussis component. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/239 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/239/239

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 8 2 2009 06 15 95 98 Kiani Zahra Department of Immunology, Chabahar International University of Medical Sciences, Chabahar, Iran Tavakkol-Afshari Jalil Department of Immunogenetic, Bu-Ali Research Center, Mashhad University of Medical Sciences, Mashhad Hojjat Hamed Department of Immunogenetic, Bu-Ali Research Center, Mashhad University of Medical Sciences, Mashhad Arab Hamid Reza Department of Periodontology, Mashhad University of Medical Sciences, Mashhad, Iran Radvar Mehrdad Department of Periodontology, Mashhad University of Medical Sciences, Mashhad, Iran Sadeghizadeh Mehrdad Department of Molecular Genetic, Tariat Modarres University, Tehran, Iran Ebadian Ahmad Reza Department of Immunogenetic, Bu-Ali Research Center, Mashhad University of Medical Sciences, Mashhad 2015 10 01 Adult periodontitis is a complex multifactorial disease whose etiology is not well defined. The pro-inflammatory and bone resorption properties of Interleukine-1α (IL-1α) strongly suggest a role for this cytokine in the pathogenesis of periodontal disease. Eighty Iranian adult patients with periodontitis and 80 Iranian controls were investigated in this study. In this study we report that the frequency of IL-1α genotypes including allele 2 of the IL-1α(-889) restriction fragment length bi-allele polymorphism were significantly increased in patients with advanced aggressive adult periodontitis compared to those with early and moderate disease. Furthermore, allele 2 was associated with increased production of IL-1α by activated peripheral blood polymorphonuclear cells of patients with advanced disease, although this increase failed to reach statistical significance. Finally, the data obtained revealed significant linkage disequilibrium between allele 2of the IL-1α (-889) polymorphism and allele 2 of the bi-allelic IL-1β (+3953) polymorphism in both patients and orally normal controls. These findings provide new insight into the possible rate of IL-1α and β genes polymorphism in the susceptibility to adult periodontitis. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/240 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/240/240

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 8 2 2009 06 15 99 106 Ramyar Asghar Department of Hematology and Oncology of Children's Medical Center, Tehran University of Medical Sci Shafiei Masoud Department of Hematology and Oncology of Children's Medical Center, Tehran University of Medical Sci Rezaei Nima Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Ir Asgarian-Omran Hossein Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Ir Abdar Esfahani Shadi Department of Hematology and Oncology of Children's Medical Center, Tehran University of Medical Sci Moazzami Kasra of Hematology and Oncology of Children's Medical Center, Tehran University of Medical Sciences, Tehr Sarafnejad Abdolfattah Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Ir Aghamohammadi Asghar Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Ir 2015 10 01 Acute lymphoblastic leukemia (ALL) is a malignant disorder of lymphoid precursor cells, which could be classified according to morphological and cytochemical methods as well as immunophenotyping. Twenty patients with ALL, who had been referred to the Children's Medical Center Hospital, during the year 2007, were enrolled in this study in order to evaluate the morphologic and immunophenotypic profile of these patients. Cytologic analysis of blood and bone marrow samples revealed that the frequency of ALL-L1 was 70%, followed by ALL-L2 and ALL-L3. The onset age of the patients with ALL-L1 was significantly lower than the patients with L2/L3. Severe anemia was significantly detected more in L1 group. Flow cytometic study of bone marrow showed that 10 cases had Pre-B1 ALL and 7 cases had Pre-B2 ALL, while three cases had Pro-B ALL. Comparisons of the characteristics and clinical manifestations among these groups did not show any appreciable difference. There were an increase percentage of CD20+ cells and a decrease CD10+ cells in pre-B2 group in comparison with pre-B1 group. Fifteen patients were in standard risk and five were in high risk. Although standard risk patients were more common in the group of pre-B1, this was not significant. Our results confirm the previous reports indicating heterogeneity of ALL. Immunophenotyping is not the only diagnostic test of importance, while morphological assessment still can be used in the diagnosis and classification of the disease. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/241 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/241/241

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 8 2 2009 06 15 107 110 Boskabady Mohammad Hosein Department of Physiology and Pharmacological, Research Centre of Medical Plants, School of Medicine, Taheri Ehsan Department of Physiology and Pharmacological, Research Centre of Medical Plants, School of Medicine, Ahmadi Sina Department of Physiology and Pharmacological, Research Centre of Medical Plants, School of Medicine, Ebrahimi Kolsoumeh Department of Physiology and Pharmacological, Research Centre of Medical Plants, School of Medicine, Soudaneh Malihe Department of Physiology and Pharmacological, Research Centre of Medical Plants, School of Medicine, Mohammadi Fatemeh Department of Physiology and Pharmacological, Research Centre of Medical Plants, School of Medicine, Sabourhasanzadeh Alireza Department of Physiology and Pharmacological, Research Centre of Medical Plants, School of Medicine, 2015 10 01 Bakers are frequently exposed to various irritant chemicals during work which can induce respiratory problems. In this study, pulmonary function tests and self-reported respiratory and allergic symptoms in bakers were compared with matched control subjects. The frequency of respiratory and allergic symptoms was evaluated in a sample of 58 Iranian bakers and 58 control subjects using a questionnaire. Pulmonary function tests (PFT) were also measured in all participants. All respiratory symptoms were significantly higher in bakers than control croup (P https://ijaai.tums.ac.ir/index.php/ijaai/article/view/242 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/242/242

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 8 2 2009 06 15 111 115 Fallah Tafti Saeid Department of Internal Medicine, Masih Daneshvari Hospital, National Research Institute of Tuberculo Mokri Bahareh Department of Internal Medicine, Masih Daneshvari Hospital, National Research Institute of Tuberculo Mansoori Nahal Department of Internal Medicine, Masih Daneshvari Hospital, National Research Institute of Tuberculo 2015 10 01 Churg Strauss Syndrome (CSS) is a form of primary vasculitis that is characterized by severe eosinophilia and often granulomatous inflammation as well as history of asthma or allergy. Previously, the association between cysteinyl leukotrien receptor antagonists (LTRAs), corticosteroid withdrawal or a sudden change in its used method and CSS had been established. We report three cases that have been referred because of dyspnea, wheezing and cough with a history of allergic rhinitis and nasal polypectomy. After polypectomy, disseminated skin purpuric rashes appeared on their forelegs, abdomen and all of them had experienced neuropathic signs in their extremities. Clinical findings, marked eosinophilia in blood and skin biopsies finally led to the diagnosis of CSS. The patients have been free of symptoms after receiving prednisolone; routine examinations and blood tests have rended regular results. Here, we report a probable occurrence of an association between nasal polypectomy and CSS on the basis of our findings. Further, extended researches are required to establish this correlation. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/243 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/243/243

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 8 2 2009 06 15 117 119 Alborizi Abdolvahab Department of Microbiology, Professor Alborzi Clinical Microbiology Research Center, Shiraz Universi Hosseini-nasab Ali Department of Microbiology, Professor Alborzi Clinical Microbiology Research Center, Shiraz Universi Zeyaeian Mazyar Department of Microbiology, Professor Alborzi Clinical Microbiology Research Center, Shiraz Universi Sanaei Anahita Department of Microbiology, Professor Alborzi Clinical Microbiology Research Center, Shiraz Universi Kashef Sara Department of Immunology-Allergy, Allergy Research Center, Shiraz University of Medical Sciences, Sh 2017 05 23 2017 07 23 The aim of this study was to evaluate the associations between caregiver-reported use of medications, alcohol, cigarette and/or waterpipe (WP), and exposure to pesticides/detergents during pregnancy with childhood-onset asthma. The study design consisted of a case-control study, conducted between December 2015 and April 2016, recruited 1503 children, aged between 3-16 years old. A questionnaire assessed the sociodemographic characteristics (age, gender, education level of both parents), the family history of asthma, and other known risk factors of asthma (heating system at home, child history of recurrent otitis, humidity in the house, child went to a daycare, smoking and drinking alcohol during pregnancy, exposure to pesticides and detergents). The multivariate analysis showed that children living in North and South Lebanon and the children living in areas where pesticides are frequently used had an increased risk of asthma (ORa=1.625, CI 1.034-2.554, p=0.035, ORa=13.65, CI 3.698-50.385; p<0.001 and ORa=3.307, CI 1.848-5.918, p<0.001 respectively). Smoking WP during pregnancy and cigarette during lactation would increase the risk of asthma in children (ORa=6.11; CI 1.244-30.008; p=0.026 and ORa=3.44; CI 1.024-11.554; p=0.046 respectively). We conclude that asthma may originate from the environmental exposure to toxics such as pesticides and tobacco (cigarettes and WP) or to alcohol and prescribed medications during pregnancy and lactation. Spreading awareness by health professionals about these preventable causes can help educate the parents and children to prevent asthma and its exacerbation.  https://ijaai.tums.ac.ir/index.php/ijaai/article/view/1478 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/1478/779

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 16 6 2017 12 23 501 510 EN Maryam Moradi Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran AND Department of Immunology and Hematology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran Mohammad Rezaee Zoonoses Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran AND Department of Medical Laboratory Sciences, Faculty of Paramedical, Kurdistan University of Medical Sciences, Sanandaj, Iran Mehdi Mohammadi Department of Immunology and Hematology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran AND Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Mohammad Rezaie Department of Anatomy, Faculty of Medicine, Kurdistan University of Medical Scienc