Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 8 1 2009 03 15 1 9 Shen Yan The First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, People’s Republic of China Cheng Kan Quan The First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, People’s Republic of China Wei Chu Yi Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032, Dong Xiong Si Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032, 2015 10 01 Viral myocarditis is a common cardiovascular disease, which has greatly threatened human health. However, up to now, the pathogenesis of viral myocarditis has been unclear, which leads to the lack of its effective treatments. To investigate the role of chemokines in pathogenesis of viral myocarditis, mRNA expression for a panel of 19 chemokines detected by RT-PCR in myocardial tissue of BALB/c mice that were inoculated intraperitoneally with coxsackievirus B3. Moreover primary cultured cardiac myocytes were infected with coxsackievirus B3 following extraction of RNA, from myocytes the expression of 19 chemokines was detected by by RT-PCR. Our results showed that there was much difference in the expression pattern of chemokines in myocardial tissue between infected mice with viral myocarditis and uninfected control mice. The expression of chemokines was varied significantly in clusters in myocardium post coxsackievirus B3 infection. There were also complexity and imbalance in the change of the expression of chemokines. In the meantime, Coxsackievirus B3 infection also influenced the expression pattern of chemokines in cardiac myocytes in vitro. However the expression of monocyte chemoattractant protein-1 alone was upregulated in cardiac myocytes post coxsackievirus B3 infection in the 19 detected chemokines. The chemokine expression pattern changed in complexity and imbalance manner both in myocardium and in primary cultured cardiac myocytes after coxsackievirus B3 infection. Coxsackievirus B3 infection may start viral myocarditis by regulating the expression pattern of chemokines in cardiac myocytes. MCP-1 may be one of key chemokines in the initial stage of viral myocarditis. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/227 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/227/227

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 8 1 2009 03 15 11 18 Q. Alenzi Faris Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, 2015 10 01 Insulin dependent diabetes mellitus (IDDM) results from irreversible loss of beta cells (β-cells) of the pancreas. A Streptozotocin (STZ)-induced diabetes in animal model mimics, in some aspects, recent onset IDDM. This study was conducted to investigate the effect of nicotinamide on experimentally-induced IDDM. Thirty Spraque Dawley rats were divided into 3 groups; a control group, a diabetic group which received an intraperitoneal (i.p.) injection of 55 mg/kg STZ and a nicotinamide group (1g/kg/day) which were dosed orally for 3 days followed by (i.p.) STZ (55 mg/kg) with the nicotinamide treatment continuing for an additional 14 days. Rats receiving STZ became diabetic after 2 weeks. This diabetic group showed hyperglycemia, and a very low level of C-peptide. Furthermore, pancreatic islets exhibited increased nitric oxide (NO) production together with an increased apoptotic index (as detected by TUNEL and electron microscopy). Nicotinamide treatment prevented STZ-induced diabetes, it also antagonized an increase in NO, and inhibited β-cell apoptosis. Fasting blood glucose, serum insulin and serum C-peptide were all within the normal range in the nicotinamide group. The nicotinamide protection of β-cells may be facilitated via inhibition of apoptosis and nitric oxide generation. It is suggested that nicotinamide might be considered an effective agent for the prevention and treatment of IDDM in prediabetic, and early stages, of IDDM. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/228 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/228/228

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 8 1 2009 03 15 19 24 Yaraee Roya Department of Immunology, Immunoregulation Research Group, Medical Research Center, Medical School, Ghazanfari Tooba Department of Immunology, Immunoregulation Research Group, Medical Research Center, Medical School, 2015 10 01 Transforming growth factor-beta (TGF-β) is one of the most important cytokines implicated in growth, differentiation, repair and also the pathogenesis of the lung fibrosis by its stimulatory effect on extracellular matrix deposition. Pulmonary epithelial cells are considered as a source of TGF-β in lung. Substance P (SP), as a neuroimmunomodulator has elevated levels in inflamed airways and although it has significant role in the pathogenesis of the lung fibrosis, but its effect on transforming growth factor -beta (TGF-β) production of the lung epithelial cells (and so its regulatory potential) remains unclear. In this study TGF-1 levels in supernatants of the normal (BEAS-2B) and cancerous (A549) lung epithelial cell line cultures at the presence of various concentrations of SP were examined and MTT assay was performed to evaluate cells viability. We have observed that SP (without any other stimulator) significantly augments TGF-β production of both BEAS and A54 cells and this effect is inhibited by NK1-receptor antagonist (CP-96345). We have also observed that the viability of cells did not significantly affect at the presence of SP. It can be concluded that SP can directly modulate the release of TGF-β from human bronchial epithelial cell line and thereby participates in various lung functions or pathologic conditions. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/229 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/229/229

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 8 1 2009 03 15 25 30 Abediankenari Saeid Department of Microbiology and Immunology, School of Medicine, Ghasemi Maryam 2 Department of Pathology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran 2015 10 01 Variety of positive as well as negative regulatory signals are provided by antigen presenting cell in particular by dendritic cells. In this research, we studied the capacity of dendritic cells to expand antigen-specific T regulatory cells.We also investigated the role of TGF-beta in induction inhibitory functions of dendritic cells in mixed leukocyte reactions. Dendritic cells were generated from blood CD14+ monocytes with granulocyte-Monocyte colony stimulating factor and interleukin-4 with or without TGF-beta (TGF-β-GM-DC or GM-DC). CD4+ T cell were isolated to assess lymphocyte proliferation by lymphocyte transformation test assay and the ratio of CD4+FOXp3+ CD25+ T cells were determined by fluorescene-activated cell sorter. T cell proliferation responses in GM-DC showed a significance antigen-specific proliferative response comparing with TGFβ-GM -DC. T Cell proliferation was inhibited in co-culture system containing DC-treated TGF-β. It can be suggested that the expsansion of T regulatory by TGF-β-GM-DC provides a means for antigen specific control of unwanted immune reactions. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/230 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/230/230

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 8 1 2009 03 15 31 42 Trajkov Dejan Institute of Immunobiology and Human Genetics, University School of Medicine "Ss. Kiril and Metodij" Stojkovikj Jagoda Mirkovska Clinic for Pulmoallergology, University School of Medicine "Ss. Kiril and Metodij", Skopje, Republic Petlichkovski Aleksandar Institute of Immunobiology and Human Genetics, University School of Medicine "Ss. Kiril and Metodij" Strezova Ana Institute of Immunobiology and Human Genetics, University School of Medicine "Ss. Kiril and Metodij" Mladenovska Olivija Efinska Institute of Immunobiology and Human Genetics, University School of Medicine "Ss. Kiril and Metodij" Sandevska Emilija Institute of Immunobiology and Human Genetics, University School of Medicine "Ss. Kiril and Metodij" Sibinovska Olgica Institute of Immunobiology and Human Genetics, University School of Medicine "Ss. Kiril and Metodij" Hristomanova Slavica Institute of Immunobiology and Human Genetics, University School of Medicine "Ss. Kiril and Metodij" Djulejic Eli Institute of Immunobiology and Human Genetics, University School of Medicine "Ss. Kiril and Metodij" Petrov Jordan Institute of Immunobiology and Human Genetics, University School of Medicine "Ss. Kiril and Metodij" Gogusev Jean INSERM U507, Hôpital Necker-Enfants Malades, Paris, France Spiroski Mirko Institute of Immunobiology and Human Genetics, University School of Medicine "Ss. Kiril and Metodij" 2015 10 01 The aim of this study was to examine the association of 22 cytokine gene polymorphism in Macedonians with chronic obstructive pulmonary disease (COPD). The sample of the population comprised of 301 normal respondents and 62 patients with COPD. Cytokine genotyping was performed by polymerase chain reaction with sequence-specific priming (PCR-SSP). Positive (susceptible) association was found between patient with COPD and IL-1α -889/C allele; where as negative (protective) association among was found for the following alleles IL-1β +3962/C; IL-12B -1188/A; IFNγ +874/T; IL-2 -330/G; IL-4 -1098/G and IL-4-33/C. We found positive (susceptible) association between patients with COPD and following genotypes: IL4 -33/T:T; IFNγ +874/A:A; IL-4 -1098/T:T ; IL-1α -889/C:C; IL-1β +3962/C:T; IL-12B -1188/C:C; IL-4Rα +1902/G:G; IL-10 -1082/G:G; IL-2 -330/T:T; IL-4 -590/C:C; and IL-1α -889/C:T. Negative (protective) association between patients with COPD and following genotypes was found: IFNγ +874/A:T; IL-4 -33/C:T; IL-4 -1098/G:T; IL-2 -330/G:T; IL-1β +3962/C:T; IL-4 -590/C:T; IL-10 -1082/A:G; and IL-4 -33/C:C. Positive (susceptible) association between patients with COPD and following haplotypes was found: IL-4/TCT; IL-10/ATC; and IL-2/TG, and negative (protective) association was found between the patients with COPD and haplotypes for: IL-4/TTC; and IL-4/GCC. It could be concluded that several cytokine polymorphisms are positively (susceptible), or negatively (protective) associated with COPD in Macedonians. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/231 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/231/231

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 8 1 2009 03 15 43 47 Aghamohammadi Asghar Department of Pediatrics, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Cheraghi Taher Growth and Development Research Center, Tehran University of Medical Sciences, Tehran, Iran Rezaei Nima Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, I Kanegane Hirokazu Department of Pediatrics, Graduate School of Medicine, University of Toyama, Toyama, Japan Abdollahzede Sina Growth and Development Research Center, Tehran University of Medical Sciences, Tehran, Iran Talaei-Khoei Mojtaba Growth and Development Research Center, Tehran University of Medical Sciences, Tehran, Iran Heidari Golnaz Growth and Development Research Center, Tehran University of Medical Sciences, Tehran, Iran Zandieh Fariborz Department of Pediatrics, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Moin Mostafa Department of Pediatrics, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Miyawaki Toshio Department of Pediatrics, Graduate School of Medicine, University of Toyama, Toyama, Japan 2015 10 01 X-linked Agammaglobulinemia (XLA) is a hereditary immunodeficiency, characterized by an early onset of recurrent bacterial infections, hypogammaglobulinemia and markedly reduced B lymphocytes number. In order to determine the association of neutropenia among Iranian patients with XLA, hospital records of 30 patients with confirmed XLA in Children Medical Center Hospital, were reviewed. Eight out of 30 XLA patients (26.7%) developed neutropenia during the course of the disease. In two patients, episodes of neutropenia were identified before or at the time of diagnosis of XLA. Other six patients whom were not visited regularly and did not receive periodical immunoglobulin replacement therapy experienced neutropenia after diagnosis of XLA. Neutropenia in XLA is mainly associated with infection and is resolved with intravenous immunoglobulin replacement and antibiotics therapy. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/232 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/232/232

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 8 1 2009 03 15 49 52 Agache Ioana Transylvania University, Faculty of Medicine, Department of Allergy and Clinical Immunology, Romania Duca Liliana Transylvania University, Faculty of Medicine, Department of Allergy and Clinical Immunology, Romania Anghel Mariana Brasov County Hospital, Immunology Laboratory, Romania Pamfil Gheorghe Transylvania University, Faculty of Medicine, Department of Biostatistics, Romania 2015 10 01 Several studies reported the appearance of asthma and autoimmune conditions in the same patient, but the clinical significance of this association was not yet assessed. One hundred asthmatic patients were observed for one year evolution with death, severe exacerbations, intake of > 1000 micrograms of beclometasone or equivalent (high ICS) and FEV1 decline >100 ml, in relation with ANA (ELISA), sputum and blood eosinophilia (EO), NSAID intolerance, BMI >25, chronic rhinosinusitis, smoking status and FEV1 100 ml/year). Multiple regression analysis pointed out several different independent risk factors for severe asthma evolution: for death presence of ANA (P=0.037), NSAID intolerance (P25 (P=0.046) and NSAID intolerance (P=0.017) The presence of ANA is an independent risk factbody against A subunit could be a useful tool for immunological diagnosis of STEC induced infection. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/291 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/291/291

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 10 1 2011 03 15 47 51 EN Asghar Aghamohammadi Research Center for Immunodeficiency, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran Ali Montazeri Iranian Institute for Health Sciences Research, Tehran, Iran Hassan Abolhassani Research Center for Immunodeficiency, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran Sepideh Saroukhani Research Center for Immunodeficiency, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran Sarvenaz Pourjabbar Research Center for Immunodeficiency, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran Mahmoud Tavassoli Research Center for Immunodeficiency, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran Behzad Darabi Research Center for Immunodeficiency, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran Amir Imanzadeh Research Center for Immunodeficiency, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran Nima Parvaneh Research Center for Immunodeficiency, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran Nima Rezaei Research Center for Immunodeficiency, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran AND 2015 10 01 Patients  with  primary  antibody  deficiencies  (PAD)  are  susceptible  to  recurrent  and chronic infections and a variety of complications. This study was performed to assess quality of life (QoL) of PAD patients who were under long term treatment and regular follow-up.Thirty six adults with proved diagnosis of PAD, who had received regular intravenous immunoglobulin replacement therapy, were enrolled in this study. The QoL of selected PAD patients was measured by Medical Outcomes Study 36-item Short-Form (SF-36) Health Survey questionnaire. The patients with PAD showed significantly reduced scores in physical component in comparison  with  healthy  age-sex  matched  control  subjects  (60.2±20.1  vs.  85.5±4.7, P<0.001). Mental component score was also significantly decreased in the patient's group (59.8±19.5 vs. 72.3±3.4, P=0.002). There was a reverse association between SF-36 scores and number of infections episodes (r=-0.73 P=0.003). The patients with long delay diagnosis showed significantly lower SF-36 scores (r=-0.62, P=0.003). The patients with PAD who were diagnosed timely and managed appropriately seem to have lower complications and better QoL. However, the patients with severe phenotypes and long delay in diagnosis showed lower QoL, even in medical management. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/292 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/292/292

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 10 1 2011 03 15 53 59 EN Masoumeh Firouzi Department of Biochemistry, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran AND Tissue Repair Lab, Institute of Biochemistry and Biophysics, University of Tehran AND Research Centre for Neural Repair, University of Tehran Farzaneh Sabouni Department of Biochemistry, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran Abdolkhaleg Deezagi Department of Biochemistry, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran Zahra Hassannejad Pirbasti Tissue Repair Lab, Institute of Biochemistry and Biophysics, University of Tehran AND Research Centre for Neural Repair, University of Tehran Fatemeh Poorrajab Tissue Repair Lab, Institute of Biochemistry and Biophysics, University of Tehran Vafa Rahimi-Movaghar Sina Trauma and Surgery Research Center, Tehran University of Medical Sciences, Tehran, Iran 2015 10 01 The p75 pan-neurotrophin receptor (p75NTR) plays a pivotal role in linking the immune system with the nervous system. p75NTR is required for the development of several characteristic features of allergic asthma. Also p75NTR upregulated by reactive Schwann cells after peripheral nerve injury. Moreover p75NTR and RhoA play a critical role in the regulation of apoptosis. To determine whether the designed siRNA for p75NTR can downregulates both p75NTR and Rho-A at RNA level in rats and, if so, at what magnitude, Schwann cell apoptosis occurs. Isolation and purification of neonate Schwann cells were prepared from rat sciatic nerve. Specific siRNA duplex was designed for p75NTR. To investigate the role of siRNA-mediated knockdown of p75NTR, the gene expression in p75NTR was examined with reverse transcription-polymerase chain reaction (RT-PCR) and Real-Time RT-PCR. Schwann cell apoptosis was performed by Annexin and TUNEL assays after 24 hours. Following p75NTR Transfection siRNA, p75NTR gene, compared with control, was downregulated by 73%. Without using siRNA for Rho-A, Rho-A gene was downregulated by 89% at the same time. Based on Annexin assay, apoptosis of Schwann cells occurred in siRNA+NGF and control+NGF by 16.76%±2.27 and 92.39%±1.82, respectively. TUNEL data showed that apoptosis of Schwann cells occurred in siRNA and control by 12.91%±6.39 and 78.55%±11.85, respectively. Thus, p75-siRNA downregulated both p75NTR and Rho-A at RNA level in rats and showed a role on decreased cell apoptosis compared to the controls. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/293 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/293/293

Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 10 1 2011 03 15 61 65 EN Alain. R Rodríguez-Orozco División de Posgrado, Facultad de Medicina “Dr Ignacio Chávez”, Universidad Michoacana de San Nicolás De Hidalgo, Morelia, Michoacán, México AND Instituto de Investigación Científica en Temas de Familia, Alergia e Inmunología, Morelia, Michoacán, México E. G. Kanán-Cedeño Instituto de Investigación Científica en Temas de Familia, Alergia e Inmunología, Morelia, Michoacán, México AND Facultad de Psicología, Universidad Michoacana de San Nicolás De Hidalgo, Morelia, Michoacán, México, Instituto de Investigación Científica en Temas de Familia, Alergia e Inmunología. Morelia, Michoacán, México E. Guillén Martínez Facultad de Psicología, Universidad Michoacana de San Nicolás De Hidalgo, Morelia, Michoacán, México M. J Campos Garibay Facultad de Psicología, Universidad Michoacana de San Nicolás De Hidalgo, Morelia, Michoacán, México 2015 10 01 Emotional factors and a recurrent psychosomatic env