Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 15 1 2016 01 31 20 26 EN Iman Fatemi Department of Pharmacology, School of Medicine and Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran Ali Shamsizadeh Physiology-Pharmacology Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran Ali Roohbakhsh Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran Fatemeh Ayoobi Geriatic Care Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran Mohammad Hossein Sanati Department of Medical Genetics, National Institute for Genetic Engineering and Biotechnology, Tehran, Iran Manijeh Motevalian Department of Pharmacology, School of Medicine and Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran 2015 11 02 2015 11 03 Orexin A and B are hypothalamic peptides with a wide variety of effects such as anti-inflammation and neuroprotection. Impaired function of orexin system has been reported in some neurodegenerative diseases like Parkinson, Huntington and Alzheimer. In this study, the mRNA expression levels of some hypothalamic peptides were investigated in C57BL/6 female mice with experimental autoimmune encephalomyelitis (EAE). Animals were randomly divided into two control and EAE groups. EAE was induced by administration of myelin oligodendrocyte glycoprotein (MOG) with complete Ferund’s adjuvant and pertussis toxin. Twenty-first days following immunization, mice were decapitated to remove the brains. Then, the expression profiles of prepro-orexin, orexin 1 receptors (OX1R) and orexin 2 receptors (OX2R) in hypothalamic region were assessed using real-time PCR method. In this study, we found a considerable increase in the mRNA expression of OX1R and OX2R following EAE induction in C57BL/6 mice. Elevation levels of OX1R and OX2R following EAE induction suggest that alteration in orexinergic system may involve in pathogenesis of multiple sclerosis. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/644 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/644/601