Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 14 4 2015 10 20 443 449 EN Sabir Hussain Department of Biosciences, COMSATS Institute of Information Technology, Park Road, Chak Shazad, Islamabad-44000, Pakistan. Tahir Iqbal Department of Internal Medicine, Shifa College of Medicine, Shifa International Hospital, H-8/4, Islamabad, 44000 Pakistan. Irfan Sadiq Department of Biosciences, COMSATS Institute of Information Technology, Park Road, Chak Shazad, Islamabad-44000, Pakistan. Saima Feroz Department of Biosciences, COMSATS Institute of Information Technology, Park Road, Chak Shazad, Islamabad-44000, Pakistan. Humayoon Shafique-Satti Department of Hematology/Medicine, Armed Forces Bone Marrow Transplant Centre, Rawalpindi, 46000 Pakistan. 2015 10 19 2015 10 19 Interleukin-8 (IL-8) is a well-known inflammatory chemokine and suggested to be involved in the development of acne vulgaris. This study investigates IL-8 plasma levels in acne patients and healthy controls and the molecular basis for the regulation of the IL-8 gene in a Pakistani population.Patients with acne vulgaris (n=264) and healthy individuals (n=264) were enrolled in this investigation. Plasma IL-8 levels were determined by enzyme-linked immunosorbent assay (ELISA). The genotyping for IL-8 gene was performed by polymerase chain reaction (PCR) and  restriction fragment length polymorphism (RFLP).Our data showed a statistically significant increase in IL-8 levels from acne patients compared with healthy subjects (154.2 ± 52.1 pg/mL in patients vs. 101.6 ± 33.5 pg/mL in controls, p<0.0001). The IL-8-251T>A (rs4073) polymorphism was significantly higher in patients with acne compared with the control group (p=0.013). There was a significant difference between the T and A alleles from acne cases and controls (odds ratio OR=1.6, 95%CI=1.16-2.19,  p=0.003).  Logistic-regression  analysis  showed  that  the  increased IL-8  levels,  and  the  IL-8-251T>A  polymorphism  were  significantly  associated  with acne.Our data suggest that the elevated IL-8 levels and the IL-8-251T>A polymorphism may be associated with acne vulgaris in the study population. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/592 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/592/542