Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 14 3 2015 10 18 287 291 EN Nusrat Saba Institute of Biomedical and Genetic Engineering, Islamabad, Pakistan AND Department of Biochemistry, PirMehar Ali Shah Arid Agriculture University Rawalpindi, Pakistan Osman Yusuf The Allergy and Asthma Institute of Pakistan, Islamabad, Pakistan Sadia Rehman Institute of Biomedical and Genetic Engineering, Islamabad, Pakistan Saeeda Munir Institute of Biomedical and Genetic Engineering, Islamabad, Pakistan Naghman Bashir Chest and Medical Specialist, MEDICSI, Islamabad, Pakistan Atika Mansoor Institute of Biomedical and Genetic Engineering, Islamabad, Pakistan Ghazala Raja-Kaukab Department of Biochemistry, PirMehar Ali Shah Arid Agriculture University Rawalpindi, Pakistan 2015 10 18 2015 10 18 Asthma is a chronic inflammatory and remodeling disorder of the airways, in which many cells, cellular elements, and cytokines play important roles. The role of tumor necrosis factor- α (TNF-α) in asthma is unclear in Pakistani population. The aim of this study was to assess the relationship between TNF-α -308 polymorphism and asthma.Polymorphism of TNF-α (G-308-A locus; rs 1800629) in 329 asthmatic patients and 151 healthy  controls  was  evaluated.  DNA  was  prepared  from  blood  samples  of  cases  and controls. Samples were genotyped for TNF-α 308 G/A polymorphism.There was no significant difference in the frequency of GG (OR 1.049 with 95% CI 0.68-1.63) and GA (OR 0.987 with 95% CI 0.64-1.53) genotypes of TNF-α-308. The AA genotype was absent in cases and only one AA genotype was observed in the controls.The genetic polymorphism of TNF-α does not seem to be associated with asthma in Pakistani population. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/569 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/569/454