Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 12 1 2013 03 15 68 74 EN Mozafar Mohammadi Nejad Razi Herbal Medicine Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran Eisa Salehi Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Mehrnaz Mesdaghi Department of Immunology, Mofid Children Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran Lida Atarod Vali-Asr Hospital, Tehran University of Medical Sciences, Tehran, Iran Masoud Movahedi Children Medical Center, Tehran University of Medical Sciences, Tehran, Iran Zahra Gheflati Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Tahereh Aboufazeli Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Mohammad Vodjgani Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran 2015 10 16 Allergic rhinitis  (AR) is  an  inflammatory  disorder  of  the  nasal  mucosa  with  high morbidity and prevalence. Natural killer (NK) cells might have a role in AR. We aimed to evaluate the changes of the markers and receptors on NK cells in AR patients compared to the non-atopic controls. Flow  cytometric  analysis was  used  with  double  staining  of  the  Peripheral  Blood Mononuclear Cells (PBMCs) to examine the expression of CD25 and CD69 markers, and NKG2D  and NKG2A  receptors on NK  cells of 20 patients with AR and 20 non-atopic controls. The serum total IgE level was measured by Enzyme-linked Immunosorbent Assay. The expression of CD69 antigen on NK cells in AR patients was significantly higher than that  of  healthy group  (p=0.03). No  significant changes were observed  between  CD25, NKG2D  and NKG2A expression on the surface of NK cells from healthy and AR subjects. Our study also showed that there was no significant correlation between the expression of CD69, CD25, NKG2D and NKG2A and level of serum total IgE in AR patients and normal subjects. These results indicated that the expression of CD69 antigen on NK cells of AR patients was increased. The high expression of CD69 on NK cells in AR patients suggested that these cells were activated, probably due to the cytokines secreted from allergen-stimulated T cells and activated monocytes. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/532 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/532/472