Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 12 4 2013 12 15 312 320 EN Zhen Chen Shanghai Huashan Hospital, Fudan University, No. 12, Urumqi Road (M), Shanghai 200040 China Ning Zhu Shanghai Huashan Hospital, Fudan University, No. 12, Urumqi Road (M), Shanghai 200040 China Xiaodong Chen Shanghai Huashan Hospital, Fudan University, No. 12, Urumqi Road (M), Shanghai 200040 China Yi Yang Shanghai Huashan Hospital, Fudan University, No. 12, Urumqi Road (M), Shanghai 200040 China Yiping Li Institute of Biochemistry and Cell Biology, Shanghai Institute of Life Sciences, Chinese Academy of Sciences, No. 320, Yueyang Road, Shanghai 200031 China Zhili Wu Institute of Biochemistry and Cell Biology, Shanghai Institute of Life Sciences, Chinese Academy of Sciences, No. 320, Yueyang Road, Shanghai 200031 China Shaoxin Chen Shanghai Institute of Pharmaceutical Industry, No. 1320, Peking W. Road. Shanghai 200040 China 2015 10 16 “Broussonetia papyrifera” (Chinese mulberry) pollen is an important source of allergens in regions surrounding Shanghai, China. To identify and purify major allergens from “B. papyrifera” pollen that reacted with serum antibodies from sensitized patients, “B. papyrifera” pollen was defatted, dried, and extracted proteins were separated using SP cationic exchange or Q anionic exchange columns. Serum samples from 29 allergic patients and 4 healthy controls were collected. Allergens in eluted fractions were identified by Western blot and enzyme-linked immunosorbent assays (ELISA) using serum samples. An inhibitory assay was used to verify allergen-specific antiserum specificity. Serum IgE of 2 patients reacted with a 15 kDa protein band. The protein was eluted with 0.1M NaCl from a SP cationic exchange column. Serum samples from the same patients positively reacted with ELISA plate coated with partially purified 15 kDa protein. Serum IgE of 11 patients reacted with a 72 kDa protein band. The protein was eluted with 0.3M NaCl from a Q anionic exchange column. Serum samples from five patients positively reacted with ELISA coated with partially purified 72 kDa protein. Our preliminary purifications identified two proteins of 72 kDa and 15 kDa as allergens derived from “B. papyrifera” pollen, which reacted with allergic patients’ serum IgE. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/496 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/496/548