Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 2 3 2003 09 15 155 158 H. Khorami TR. Neyestani Mehri. Kadkhodaee J. Lotfi 2015 10 01 Neopterin, apyrazinopyrimidine compound, is produced by macrophages after induction by interferon gamma (IFN-y) and serves as a marker of cellular immune system activation followed by oxidative stress. The aim of this study was to determine urinary neopterin to creatinine ratio (UNCR) as a surrogate marker of cell-mediated immune activation in multiple sclerosis (MS). Three weekly early morning urine samples were collected from 27 patients with MS and 31 age- and sex-matched apparently healthy subjects. Urinary neopterin and creatinine were determined using reversed phase high-performance liquid chromatography and Jaffe reaction, respectively. UNCR was significantly higher in patients than in healthy controls indicating IFN-y-induced cellular immunity activation and oxidative stress in multiple sclerosis. As a non-invasive method, UNCR determination may be helpful in monitoring disease progression and the effects of therapies, as well. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/49 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/49/49