Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 13 3 2014 06 15 157 165 EN Monireh Mohsenzadegan Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Fahimeh Fattahi Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran. Fatemeh Fattahi Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran AND Department of Pathology and Medical Biology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands. Abbas Mirshafiey Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Mohammad Reza Fazlollahi Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran. Fariba Naderi Beni Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Masoud Movahedi Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran AND Department of Immunology and Allergy, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran. Zahra Pourpak Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran AND Department of Immunology and Allergy, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran. 2015 10 16 Chronic  granulomatous  disease (CGD)  is a rare primary immunodeficiency disorder characterized by a greatly increased susceptibility to severe fungal and bacterial infections caused  by  defects  in  NADPH   oxidase  of  phagocytic  cells. We  aimed  to  investigate immunophenotype alterations of naïve and memory B cells and B1a cells in peripheral whole blood from Iranian patients with CGD. Flow cytometric analysis was performed  on  peripheral blood  samples from  31 CGD patients and 23 healthy controls (HC) to study naïve (IgD+/CD27-), memory (CD27+) B and B1a (CD5+) cells. Soluble CD27 (sCD27) and immunoglobulins were also measured by ELISA and the nephelometric method, respectively. We found significantly higher levels of naïve B cells and B1a cells but lower levels of memory B cells in CGD patients compared to HC. There was no significant difference in soluble CD27 (sCD27) alteration between CGD patients and HC. Our findings suggested a role for NADPH oxidase in process of B cell differentiation and impairing conversion of naïve B cells to  memory B cells and altered B1a cells in CGD patients.  Increased   susceptibility  of   CGD   patients   to   opportunistic   infections   and autoimmune disorders could be partly explained by the altered phenotype of B lymphocytes in these patients. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/461 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/461/376