Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 13 5 2014 10 15 356 363 EN Ebrahim Shakiba Department of Clinical Biochemistry, Hamadan University of Medical Sciences, Hamadan, Iran. Haidar Tavilani Department of Clinical Biochemistry, Hamadan University of Medical Sciences, Hamadan, Iran. Mohamad Taghi Goodarzi Department of Clinical Biochemistry, Hamadan University of Medical Sciences, Hamadan, Iran. Amir Kiani Department of Toxicology and pharmacology, Kermanshah University of Medical Sciences, Kermanshah, Iran AND Molecular Diagnostic Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran Tayehbbeh Pourmotabbed Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, U.S.A. Asad Vaisi-Raygani Molecular Diagnostic Research Center, AND Department of Clinical Biochemistry, Kermanshah University of Medical Sciences, Kermanshah, Iran. 2015 10 16 Evidences indicate that angiogenesis  is an important process in the development of destructive synovial tissue in rheumatoid  arthritis (RA). Recently, it has been shown that the polymorphism of the integrin-αv subunit encoded by the ITGAV gene plays a role in angiogenesis and is considered  as RA susceptibility loci. This study investigated association of four single nucleotide polymorphisms  (SNPs) in ITGAV with  disease  activity  score (DAS28), serum levels  of  C-reactive protein  (CRP), and  anti-cyclic citrullinated peptide(anti-CCP) antibody in 419 RA patients and 398 healthy individuals. Four SNPs in  ITGAV  gene  (rs3911238, rs3738919, rs10174098 and  rs3768777) were  analyzed.   Serum concentrations of anti-CCP antibody and CRP were measured by ELISA. We used the EULAR activity criteria to calculate DAS28-CRP. Among these SNPs, the ITGAV-rs3911238-G/C polymorphism was associated with RA disease activity  [remission-to-low   and  moderate-to-high  in  codominant   model  (CC vs.GG:  OR=1.53, p=0.041 and allele (C vs. G: OR=1.18, p=0.042)] and presence of anti-CCP (codominant CC vs.GG: OR=2.77, p=0.001,  allele C vs. G:  OR=1.19,p=0.033).  The  carriers  of  CC genotype  ITGAV- rs3911238 had higher serum levels of CRP and anti-CCP antibody titer and higher ESR and disease activity  score than carriers of GG  and CG genotypes. Furthermore, haplotypes  analysis showed that ITGAV rs3733891C/rs3768777T/rs3911238C/rs10174098A and ITGAV rs3733891A/rs3768777T/rs3911238G/rs10174098A haplotypes increased severity and anti-CCP antibody in RA patients (OR=5.54, p=0.049 and OR=2.89;  p=0.024, respectively) in comparison with ITGAV rs3733891C/rs3768777T/rs3911238G/rs10174098A haplotypes. Thus, the present study demonstrated that the link between systemic inflammatory markers and the ITGAV-rs3911238 polymorphism  allele in Iranian RA patients.     https://ijaai.tums.ac.ir/index.php/ijaai/article/view/433 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/433/404