Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 24 1 2025 02 13 52 60 Fatemeh Sabaghi Department of Hematology and Blood Banking, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran AND Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran Minoo Shahidi Department of Hematology and Blood Banking, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran Majid Safa Department of Hematology and Blood Banking, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran Mohammad Faranoush Pediatric Growth and Development Research Center, Institute of Endocrinology, Iran University of Medical Sciences Tehran, Iran Mostafa Jamali Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran Ebadollah Salekmoghadam Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran Fatemeh Mohamadali Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran Shahin Sharifi Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran Saeed Mohammadi Research Institute for Oncology, Hematology and Cell Therapy, Tehran University of Medical Sciences, Tehran, Iran Reza Golestani Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran 2024 05 06 2024 08 19 Administering human leukocyte antigen (HLA)-compatible platelets is a tactic for treating patients with poor responses to random platelet injections. HLA-matched platelet provision requires many donors with HLA-typed and organized information. This study, the first of its kind in Iran, aimed to develop a registry system of HLA-typed platelet donors to facilitate the provision of compatible platelets to patients, leveraging the diversity of HLA alleles across Iran's various provinces. This study involved the HLA-typing of 1850 plateletpheresis donors, who were also registered as unrelated stem cell donors, across all blood centers in Iran from 2015 to 2022. HLA-A and HLA-B genotyping was conducted at a low-resolution using polymerase chain reaction-sequence specific primers (PCR-SSP) and real-time PCR. Statistical analysis was performed to determine allelic genotypes and donor profiles. The majority of the donors were male (99.7%), with a mean age of 36 years. The high donor rate in Tehran indicates a larger pool of potential HLA-platelet donors due to a denser population and more donation facilities. The donors were recruited for HLA-compatible plateletpheresis. The frequency of HLA-AB alleles among donors was relatively consistent with those documented by Iranians. Our findings can be utilized to create a foundational HLA database. A registry system for HLA-typed platelet donors is crucial due to high HLA polymorphism and ethnic diversity. This system facilitates the rapid identification of compatible donors based on HLA typing. Additional inquiries are needed to expand the plateletpheresis registry and make a request-supply mechanism between the Iranian Blood Transfusion Organization and hospitals. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/4097 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/4097/2144