Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 14 2 2015 04 15 158 167 EN Javad Arasteh Department of Biology, Faculty of Basic Sciences, Islamic Azad University Central Tehran Branch, Tehran, Iran. Massoumeh Ebtekar Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Zahra Pourpak Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran. Ali Akbar Pourfatollah Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Zuhair Mohammad Hassan Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Gholam Ali Kardar Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran. Ahad Zare Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran. Shiva Saghafi Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran. Agheel Tabar Molla Hassan Department of Immunology, Faculty of Medical Sciences,, Islamic Azad University Babol Branch, Babol, Iran. 2015 10 16 Cytokines have been introduced as critical inducers in the development of Th subpopulations.Cytokines like IL-10 are involved in inducing regulatory T cells such as Type 1 regulatory T (Tr1) cells cells. IL-22 is a member of IL-10 family of cytokines, and IL-28A is a member of IFN-γ family. In this study, cord blood mononuclear cells (CBMC) from normal healthy individuals were isolated by Ficoll and then naïve T cells were purified by CD4+CD25+ Regulatory T cell Isolation kit. The effect of these two cytokines on production of IL-5, TGF-β, IL-10, IL-4 and IFN-γ cytokines from cord blood T cells was investigated to identify Tr1 cells as well as Th1 and Th2 polarization. Flow cytometric analysis showed that IL-28A and IL-22 were not effective in expression of IL-5 and TGF-β either alone or in synergy, but in view of IL-10, IL-4 and IFN-γ, the results showed that IL-22 increased IL-10 and IL-4 but had a decreasing effect on IFN-γ. The results showed that IL-28A was not effective in increasing or decreasing the level of IL-10, IL-4 and IFN-γ. Therefore, according to these results, IL-22 and IL-28A were not effective in inducing Tr1 cells. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/398 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/398/439