Tehran University of Medical Sciences Iranian Journal of Allergy, Asthma and Immunology 1735-1502 23 3 2024 05 27 321 329 Samira Karbasi Cardiovascular Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran Nafiseh Erfanian Student Research Committee, Department of Molecular Medicine, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran Hamideh Dehghan Student Research Committee, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran Asghar Zarban Cardiovascular Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran AND Department of Clinical Biochemistry, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran mahammad hasan Namaei Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran Mohammad Yahya Hanafi-Bojd Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran Saeed nasseri Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran 2023 07 16 2024 01 22 Today, camel milk consumption in the Middle East is trendy because it is believed that it reduces the risk of cancer. Recently, studies have discovered that most of milk's beneficial effects are because of its nanoparticles, especially exosomes. The objective of the present research was to investigate the anti-cancer effects of camel milk exosomes (CMEXOs) in the murine colorectal cancer cell line (CT-26). Our findings verified the existence of exosomes measuring approximately 114.1±3.4 nm in diameter. Through MTT and migration assays, we established that CMEXOs exhibit dose-dependent anti-proliferative and anti-migration effects on the CT-26 cell line. Furthermore, our study showed that treatment with CMEXOs led to a reduction in TNF-α and IL-6 gene expression in CT-26 cells. While additional in vivo studies are required, our data demonstrate that CMEXOs have anti-proliferative and anti-migration effects on CT-26, possibly by influencing crucial genes within the inflammation pathway. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/3874 https://ijaai.tums.ac.ir/index.php/ijaai/article/download/3874/2078